Real-Time in Situ Visualizing of the Sequential Activation of Caspase Cascade Using a Multicolor Gold–Selenium Bonding Fluorescent Nanoprobe

Liu, Xiaojun; Song, Xiaoxiao; Luan, Dongrui; Hu, Bo; Xu, Kehua; Tang, Bo

doi:10.1021/acs.analchem.9b00452

Cited by 43 publications

(29 citation statements)

References 42 publications

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“…The selenium-functionalized systems showed increased SNR during in vitro tests in MCF-7 cells and serum, showing the potential of this strategy in the development of highly stable and reproducible gold-based nanosensors. Furthermore, the authors developed a multiplexing sensing system to study protease cascades in vitro that was able to discriminate between the activity of Caspase-3 (executioner) and that of -8 and -9 (initiators) with minimal background and nonspecific signal in real time [40,41].…”

Section: Looking For Activity In Real Time: How Is It Done?mentioning

confidence: 99%

Monitoring Proteolytic Activity in Real Time: A New World of Opportunities for Biosensors

Oliveira-Silva

Sousa-Jerónimo

Botequim

et al. 2020

Trends in Biochemical Sciences

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Proteases play a pivotal role in several biological processes, from digestion, cell proliferation, and differentiation to fertility. Deregulation of protease metabolism can result in several pathological conditions (i.e., cancer, neurodegenerative disorders, and others). Therefore, monitoring proteolytic activity in real time could have a fundamental role in the early diagnosis of these diseases. Herein, the main approaches used to develop biosensors for monitoring proteolytic activity are reviewed. A comparison of the advantages and disadvantages of each approach is provided along with a discussion of their importance and promising opportunities for the early diagnosis of severe diseases. This new era of biosensors can be characterized by the ability to control and monitor biological processes, ultimately improving the potential of personalized medicine. Biosensing in Personalized Medicine: The Emergence of Protease BiomarkersA deep understanding of the individual characteristics of biological processes and precise control of responses and feedback to therapies are essential in a world that is running towards personalized medicine (see Glossary). Recent reports predict that this market will grow at a compound annual growth rate (CAGR) of over 11% from US$92.4 billion dollars in 2017 to US$194.4 billion in 2024 (https://www.researchandmarkets.com/research/n5kqz7/global?w=5), with oncology responsible for 30% of the revenues generated. Biosensors are one of the underlying technologies of personalized medicine, giving support to the highly informed decisions that are critical to obtain better clinical outcomes and to decrease undesired side-effects. Compared with the personalized medicine market, a similar tendency is observed for the biosensors market where growth from US$18.6 billion dollars in 2018 to US$31.5 billion in 2025 (8% CAGR) is expected (https://www.gminsights.com/industry-analysis/biosensors-market).One of the primary goals of a biosensor is to determine the presence/absence or activity of a given biomarker and to correlate these results with pathophysiological conditions. Proteases are emerging as a new category among the wide variety of biomarkers targeted by biosensors due to their key role in health and disease. In this review, we present the main approaches used to measure proteolytic activity in real timefor both in vivo and in vitro applicationsand discuss recent developments in and future possibilities for protease biosensors (Figure 1A). The Roles and Idiosyncrasies of Proteases: Why Monitor Proteolytic Activity?Proteases are a widely explored research topic as illustrated by more than 487 000 entries registered in PUBMED. However, it was only recently that proteases emerged as an analyte of interest for biosensing [68]. These hydrolytic enzymes (EC 3.4) play a pivotal role in several biological processes, such as digestion, cell proliferation, and differentiation [1], as well as apoptosis [2], coagulation [3], immunity [4], and fertility [5]. The deregulation of protease metabolism can resul...

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Section: Looking For Activity In Real Time: How Is It Done?mentioning

confidence: 99%

Monitoring Proteolytic Activity in Real Time: A New World of Opportunities for Biosensors

Oliveira-Silva

Sousa-Jerónimo

Botequim

et al. 2020

Trends in Biochemical Sciences

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“…This nanoprobe is a promising tool for real-time monitoring of caspase cascade activations. 47 Based on this work, the Tang group also developed another Au-Se-bonded nanoprobe (8) for uorescence imaging of caspase-3 and cathepsin B (Fig. 8b).…”

Section: Cancermentioning

confidence: 99%

Fluorescent probes for visualizing ROS-associated proteins in disease

Wang

et al. 2021

Chem. Sci.

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“…There are clear charts showing the probe, and probe with caspase, followed by probe, caspase and inhibitor. The authors underscored to the scientific community the finding that caspase is a cascade that can serve as a target for molecular probing that can be imaged [156].…”

Section: A Recent Report About Reductants and Ros In The Context Of Probing And Fluorescencementioning

confidence: 99%

Discussions of Fluorescence in Selenium Chemistry: Recently Reported Probes, Particles, and a Clearer Biological Knowledge

et al. 2021

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In this review from literature appearing over about the past 5 years, we focus on selected selenide reports and related chemistry; we aimed for a digestible, relevant, review intended to be usefully interconnected within the realm of fluorescence and selenium chemistry. Tellurium is mentioned where relevant. Topics include selenium in physics and surfaces, nanoscience, sensing and fluorescence, quantum dots and nanoparticles, Au and oxide nanoparticles quantum dot based, coatings and catalyst poisons, thin film, and aspects of solar energy conversion. Chemosensing is covered, whether small molecule or nanoparticle based, relating to metal ion analytes, H2S, as well as analyte sulfane (biothiols—including glutathione). We cover recent reports of probing and fluorescence when they deal with redox biology aspects. Selenium in therapeutics, medicinal chemistry and skeleton cores is covered. Selenium serves as a constituent for some small molecule sensors and probes. Typically, the selenium is part of the reactive, or active site of the probe; in other cases, it is featured as the analyte, either as a reduced or oxidized form of selenium. Free radicals and ROS are also mentioned; aggregation strategies are treated in some places. Also, the relationship between reduced selenium and oxidized selenium is developed.

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Real-Time in Situ Visualizing of the Sequential Activation of Caspase Cascade Using a Multicolor Gold–Selenium Bonding Fluorescent Nanoprobe

Cited by 43 publications

References 42 publications

Monitoring Proteolytic Activity in Real Time: A New World of Opportunities for Biosensors

Monitoring Proteolytic Activity in Real Time: A New World of Opportunities for Biosensors

Fluorescent probes for visualizing ROS-associated proteins in disease

Discussions of Fluorescence in Selenium Chemistry: Recently Reported Probes, Particles, and a Clearer Biological Knowledge

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