Real-Time Evaluation of Live Cancer Cells by an <i>in Situ</i> Surface Plasmon Resonance and Electrochemical Study

Wu, Chang-Yu; Rehman, Fawad Ur; Li, Jingyuan; Ye, Jing; Zhang, Yuanyuan; Su, Meina; Jiang, Hui; Wang, Xuemei

doi:10.1021/acsami.5b08066

Cited by 31 publications

(15 citation statements)

References 40 publications

(52 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Herring sperm DNA (hsDNA) was exposed to the immobilized bacteria, which was shown to adsorb to E. coli cell surfaces with kinetic parameters that were in agreement with those from other studies [114]. Wu et al recently applied an alternative approach toward the study of live cancer cells by SPR and CV, where HepG2 cells were cultured directly on gold sensor chips prior to insertion into the SPR instrument [115]. When treated with danorubicin, notable morphology and mass changes led to shifts in the SPR signal, while in situ CV was used to monitor danorubicin uptake from the culture medium.…”

Section: Hyphenated Assays With Surface Plasmon Resonancesupporting

confidence: 52%

Bioinspired assemblies and plasmonic interfaces for electrochemical biosensing

Hinman

Cheng

2016

Journal of Electroanalytical Chemistry

View full text Add to dashboard Cite

Electrochemical biosensing represents a collection of techniques that may be utilized for capture and detection of biomolecules in both simple and complex media. While the instrumentation and technological aspects play important roles in detection capabilities, the interfacial design aspects are of equal importance, and often, those inspired by nature produce the best results. This review highlights recent material designs, recognition schemes, and method developments as they relate to targeted electrochemical analysis for biological systems. This includes the design of electrodes functionalized with peptides, proteins, nucleic acids, and lipid membranes, along with nanoparticle mediated signal amplification mechanisms. The topic of hyphenated surface plasmon resonance assays is also discussed, as this technique may be performed concurrently with complementary and/or confirmatory measurements. Together, smart materials and experimental designs will continue to pave the way for complete biomolecular analyses of complex and technically challenging systems.

show abstract

Section: Hyphenated Assays With Surface Plasmon Resonancesupporting

confidence: 52%

Bioinspired assemblies and plasmonic interfaces for electrochemical biosensing

Hinman

Cheng

2016

Journal of Electroanalytical Chemistry

View full text Add to dashboard Cite

show abstract

“…In comparison with traditional detection methods, like X-ray, HPLC, and HPLC-MS, SPR technology can perform kinetics parameters (association and dissociation process) measurement, concentration measurement, and real-time molecule detection. It has been widely used in chemical, biology, agriculture, environment, and food safety [6][7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Angle-Scanning Surface Plasmon Resonance System with 3D Printed Components for Biorecognition Investigation

Zhang

Settu

Liu

2018

Advances in Condensed Matter Physics

View full text Add to dashboard Cite

Surface plasmon resonance (SPR) is a real-time, label-free, and high-sensitive detection technology. SPR has been widely used in many applications such as biomolecular interaction analysis, environmental monitoring, and medical diagnostics. However, conventional SPR sensor systems usually require expensive equipment and complicated optics. In this paper, we have demonstrated a rapid prototyping of angle-scanning SPR for bioanalytical investigation. Rapid prototyping was attained by utilizing the FDM (fused deposition modeling) based 3D (three-dimensional) printing technology. Two rotating platforms were employed to drive the laser source and photodiode, respectively. A temperature regulation unit was incorporated to maintain the system temperature in order to reduce the temperature effect. The proposed SPR rapid prototyping yielded a refractive index resolution of 6.4×10 −6 RIU (refractive index unit), and the biotin-avidin system validated the kinetics parameters measurement capability. The obtained results indicated that the FDM 3D printing has great potential for developing rapid-prototyping SPR system.

show abstract

“…For example, simple double potential (ΔV d ) could induced SPR angle shift (Δθ SPR ) via sensing film changes in the refractive index (Δn), changes in the average adsorbed monolayer thickness (Δd s ), and surface charge density (Δn e ) of the sensing film. Among these factors, the relationship between Δn e and the interface property of Δd s = -C d (ΔV d /e Δn e ), where C d is the double layer capacitance, the CV-SPR signal was proportional to the potential-induced SPR angle shift given by55,56…”

Section: Resultsmentioning

confidence: 99%

<p>High-affinity carboxyl-graphene oxide-based SPR aptasensor for the detection of hCG protein in clinical serum samples</p>

Chiu¹,

Kuo²,

Chen³

2019

IJN

View full text Add to dashboard Cite

Background The use of functionalized graphene oxide (fGO) has led to a new trend in the sensor field, owing to its high sensitivity with regards to sensing characteristics and easy synthesis procedures. Methods In this study, we developed an ultra-sensitive carboxyl-graphene oxide (carboxyl-GO)-based surface plasmon resonance (SPR) aptasensor using peptides to detect human chorionic gonadotropin (hCG) in clinical serum samples. The carboxyl-GO based SPR aptasensor provided high affinity and stronger binding of peptides, which are great importance to allow for a non-immunological label-free mechanism. Also, it allows the detection of low concentrations of hCG, which are in turn considered to be important clinical parameters to diagnose ectopic pregnancies and paraneoplastic syndromes. Results The high selectivity of the carboxyl-GO-based SPR aptasensor for hCG recombinant protein was verified by the addition of the interfering proteins bovine serum albumin (BSA) and human serum albumin (HSA), which did not affect the sensitivity of the sensor. The carboxyl-GO-based chip can enhance the assay efficacy of interactions between peptides and had a high affinity binding for a k a of 17×10 6 M −1 S −1 . The limit of detection for hCG in clinical serum samples was 1.15 pg/mL Conclusion The results of this study demonstrated that the carboxyl-GO-based SPR aptasensor had excellent sensitivity, affinity and selectivity, and thus the potential to be used as disease-related biomarker assay to allow for an early diagnosis, and possibly a new area in the field of biochemical sensing technology.

show abstract

Real-Time Evaluation of Live Cancer Cells by an in Situ Surface Plasmon Resonance and Electrochemical Study

Cited by 31 publications

References 40 publications

Bioinspired assemblies and plasmonic interfaces for electrochemical biosensing

Bioinspired assemblies and plasmonic interfaces for electrochemical biosensing

Angle-Scanning Surface Plasmon Resonance System with 3D Printed Components for Biorecognition Investigation

<p>High-affinity carboxyl-graphene oxide-based SPR aptasensor for the detection of hCG protein in clinical serum samples</p>

Contact Info

Product

Resources

About