Reaginic Antibody Formation in Mice Injected with Rabbit Anti-Mouse Thymocyte Serum

Kind, Leon S.; Macedo-Sobrinho, Braz

doi:10.1159/000231077

Cited by 15 publications

(6 citation statements)

References 8 publications

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“…It has been reported that the carrier-specific helper cells for the IgE and IgG 1 antibody response are different (20,50). Since atrophy of the spleen was already severe 5 days after injection of DNT (47) the results obtained indicate, as has been shown in splenectomized animals (19,29,39,50), that the spleen may not be an important organ in the production of IgE but is important in the production of IgG1. When DNT was inactivated by heat, it no longer had any effect on the spleen or on the adjuvanticity of pertussigen (data not shown).…”

Section: Discussionmentioning

confidence: 81%

Effects of Bordetella pertusiss Components on IgE and IgG1 Responses

Sekiya

1983

Microbiology and Immunology

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The effect of dermonecrotic toxin (DNT), fimbrial hemagglutinin (FHA), K-agglutinogen, lipopolysaccharide (LPS), and pertussigen from Bordetella pertussis on the production of IgE and IgGI antibodies to hen egg albumin (Ea) was investigated in C57BL/6 mice. The IgE antibody contents were determined by passive cutaneous anaphylaxis (PCA) in the skin of Lewis rats, while the IgG I antibody contents were determined by PCA reactions on the skin of mice using sera that had been heated for 3 hr at 56 C to destroy the IgE antibodies. Among the B. pertussis components tested, pertussigen was the most effective adjuvant for increasing the IgE and IgG I antibodies to Ea. LPS also moderately increased both types of antibodies, and FHA slightly increased the IgGI titers. When LPS was given 5 days before Ea, it suppressed both IgE and IgGI titers while FHA had only slight adjuvant action on both type of antibodies.When each of the components was tested for its ability to modify the adjuvant action of pertussigen, it was found that only DNT interfered significantly with the adjuvanticity ofpertussigen when given on the day of immunization with Ea. When the components were given 5 days before Ea, DNT produced significant suppression of only the IgGI response. LPS, FHA, and K-agglutinogen did not significantly affect the adjuvant action of pertussigen, B. pertussis cells excert an adjuvant effect on the production of antibodies in man and animals (32). This ability is shared by the lipopolysaccharides (LPS) of most gram-negative bacteria (26), but the adjuvant action of B. pertussis is due not entirely to LPS, but also to a protein called pertussigen by Munoz and coworkers (32) which has been referred to as a leukocytosis-promoting factor (LPF), leukocytosis-promoting factor-hemagglutinin (LPF-HA), histamine-sensitizing factor (HSF), and islet-activating protein (lAP) (6,34,45,51). Pertussigen has the unique property of enhancing the IgE class of immunoglobulins, an effect first observed by Mota (28) employing whole-cell pertussis vaccine. Many investigators have since confirmed the adjuvant action of pertussis vaccine and pertussigen (32). Munoz et al (34) found that as little as 0.1 ng of highly purified crystalline pertussigen increased production of IgE and IgG 1 antibodies to hen egg albumin (Ea).

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Section: Discussionmentioning

confidence: 81%

Effects of Bordetella pertusiss Components on IgE and IgG1 Responses

Sekiya

1983

Microbiology and Immunology

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“…This lack of correlation of animal models with the allergic state in man was overcome with the demonstration by Levine and coworkers that persistent and boosterable IgE antibodies can be stim ulated in certain strains of mice by injection of low doses of antigen plus Al(OH)3 [17,18,49,50]. Following the report of these observations, oth er experimental manipulations have been reported that lead to enhanced IgE synthesis [7,14,23,28,29,31,36,[45][46][47], One such method, the use of whole cells or extracts containing the HSF of B. pertussis, has shown that persistent and boosterable titers of reaginic antibody can be obtained with high doses of antigen and adjuvant, and this response dif fers from that obtained with antigen and Al(OH), [23], These findings are extended in the present investigation. It was observed that although both AI(OH)3 and HSF stimulate hemagglutinin and reagin, Al(OH)3 usually is a better stimulator of the former while HSF is of the latter.…”

Section: Discussionmentioning

confidence: 99%

“…In addition to the Al(OH)3-induced stimulation of IgE antibody pro duction described by Levine and coworkers, other methods of augment ing IgE antibody production to a particular antigen have been described [7,14,23,24,28,29,31,36,[45][46][47]. We have recently investigated one such method, that is the enhancement of specific IgE production to an an tigen by treatment of mice with extracts of the histamine-sensitizing factor (HSF) of Bordetella pertussis [23].…”

Section: Introductionmentioning

confidence: 99%

Adjuvant Activity of the Histamine-Sensitizing Factor of <i>Bordetella pertussis</i> in Different Strains of Mice

Lehrer¹,

Vaughan²,

Tan³

1975

Int Arch Allergy Immunol

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The effect of an extract of histamine-sensitizing factor (HSF) of Bordetella pertussis on the immune response of different strains of mice to ovalbumin (OA) was investigated with regard to optimal dose of antigen and adjuvant. It was observed that all strains of mice treated with HSF during immunization with OA demonstrated enhanced production of hemagglutinating antibodies, as compared to animals treated with antigen alone. This enhancement was generally not as great as that demonstrated when Al(OH)₃ was the adjuvant. HSF also stimulated a reaginic antibody response (IgE) to OA, but not in all strains of mice. In reagin responders optimal responses were observed with high doses of both antigen and adjuvant, whereas low doses of both produced little or no response. Maximal reagin production occurred usually 14–28 days after immunization and persisted for long periods of time. An anamnestic reagin response was elicited upon secondary immunization with antigen alone, not only in mice immunized with OA and HSF but also in animals treated with OA alone. These studies demonstrate the profound effect that a microbial substance such as HSF can have on reaginic antibody production and suggest that the stimulation of IgE antibody production is the net result of a number of factors including genetic capabilities of the host, environmental influence such as adjuvants, and prior exposure to an antigen.

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“…Since concanavalin A (Con A) is a potent T cell mitogen, and both in hibits and enhances the formation of non-IgE antibodies in vivo [5,7,13] and in vitro [3,20,22,26], it was decided to investigate the effect of Con A on reaginic antibody formation in mice. We had previously shown that rabbit antimouse thymocyte serum (RAMTS) induced the formation of reagins in mice to serum proteins present in RAMTS [11],…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Reaginic Antibody Formation in the Mouse by Concanavalin A

Gollapudi¹,

Kind²

1975

Int Arch Allergy Immunol

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SWR mice injected with Concanavalin A (Con A) and Ovalbumin (Ov) one immediately after the other or with mixtures of Con A and Ov produced reaginic antibodies to Ov (titer 1:80) after primary immunization and reaginic antibody levels of 1:640 after secondary immunization. Mixtures of Con A and Ov were shown to contain soluble complexes which could be precipitated by 20% polyethylene glycol (PEG). Mixtures of Con A and human serum albumin or Con A. and bovine serum albumin did not form soluble complexes and did not induce detectable reagin formation. The possible role of soluble Con A-Ov complexes in stimulating reaginic antibody is discussed.

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Reaginic Antibody Formation in Mice Injected with Rabbit Anti-Mouse Thymocyte Serum

Cited by 15 publications

References 8 publications

Effects of Bordetella pertusiss Components on IgE and IgG1 Responses

Effects of Bordetella pertusiss Components on IgE and IgG1 Responses

Adjuvant Activity of the Histamine-Sensitizing Factor of <i>Bordetella pertussis</i> in Different Strains of Mice

Enhancement of Reaginic Antibody Formation in the Mouse by Concanavalin A

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