Adjuvant Activity of the Histamine-Sensitizing Factor of <i>Bordetella pertussis</i> in Different Strains of Mice

Abstract

The effect of an extract of histamine-sensitizing factor (HSF) of Bordetella pertussis on the immune response of different strains of mice to ovalbumin (OA) was investigated with regard to optimal dose of antigen and adjuvant. It was observed that all strains of mice treated with HSF during immunization with OA demonstrated enhanced production of hemagglutinating antibodies, as compared to animals treated with antigen alone. This enhancement was generally not as great as that demonstrated when Al(OH)₃ was the adjuvant. HSF also stimulated a reaginic antibody response (IgE) to OA, but not in all strains of mice. In reagin responders optimal responses were observed with high doses of both antigen and adjuvant, whereas low doses of both produced little or no response. Maximal reagin production occurred usually 14–28 days after immunization and persisted for long periods of time. An anamnestic reagin response was elicited upon secondary immunization with antigen alone, not only in mice immunized with OA and HSF but also in animals treated with OA alone. These studies demonstrate the profound effect that a microbial substance such as HSF can have on reaginic antibody production and suggest that the stimulation of IgE antibody production is the net result of a number of factors including genetic capabilities of the host, environmental influence such as adjuvants, and prior exposure to an antigen.

Soy- and Shrimp-Specific IgE Responses in Orally and Intraperitoneally Immunized Mice

“…For example, certain strains of rats [21] and mice [24,25] are genetically predisposed to produce IgE in response to protein antigens. In the mouse, these differences may be related to histocompatibility type [26].…”

Molecular Pathogenesis, Epidemiology, and Clinical Manifestations of Respiratory Infections Due toBordetella pertussisand OtherBordetellaSubspecies

“…This is probably due to the presence of transplacentally acquired antibody to PT. PT is a strong adjuvant in several immunologic systems in several animals and humans (14,15,190,235,246,452,453,456,553,561,573,575,578,600,610,649,699,745,746,801,804). This adjuvancy in the experimental-animal model is associated with enhancement of serum antibody responses to other antigens, increased cellular immune responses to various protein antigens, contribution to hyperacute experimental autoallergic encephalomyelitis, and increased anaphylactic sensitivity.…”

Allergenicity of Concanavalin A in Mice

“…4) are intermediate responders and C57B1/6 and BALB/c are low responders. Regarding the mouse strains which have been tested in both the Con A and L. perenne pollen sys tems, the data are sufficiently similar to warrant the conclusion that the major genet ic effect is not antigen determinant-specific but appears to be expressed at the level of the amount of reagin produced by mouse strains [19,20], The low reagin responsive ness of SJL/J mice is well known [5,12,16,19,39].…”

“…sites. Damage by tis sue migrating helminth larvae [37], inflam mation induced by the histamine-sensitizing factor (HSF) of B. pertussis [16], inflamma tion induced by mucous membrane-located helminths [38], or transport into s.c. and s.m. sites by antigen-binding cells, would all increase access of antigen.…”