Reagentless biosensors based on self-deposited redox polyelectrolyte-oxidoreductases architectures

Narváez, Arántzazu; Suárez, Guillaume; Popescu, Ionel Cătălin; Katakis, Ioanis; Domı́nguez, Elena

doi:10.1016/s0956-5663(00)00049-x

Cited by 103 publications

(57 citation statements)

References 42 publications

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“…An estimation of this influence can be done by voltammetric measurements in terms of I max /K m app ratio. These parameters are often calculated in the design of enzymatic sensors to evaluate the sensitivity of the system proposed, which is related to the low or high affinity of an enzyme towards a specific substrate [23,26]. Lower K m app values at similar catalytic currents involved higher effectiveness of the enzyme at lower mediator concentrations.…”

Section: Discussionmentioning

confidence: 99%

Voltammetric monitoring of laccase-catalysed mediated reactions

Fernández‐Sánchez

Tzanov

Gübitz

et al. 2002

Bioelectrochemistry

116

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Six different compounds capable of mediating laccase-catalysed reactions have been tested by cyclic voltammetry. They exhibited quasireversible electrodic behaviour with formal redox potentials ranging from 150 to 800 mV (E 0 Vvs. SCE). The immersion of a laccase-coated glassy carbon electrode (GCE) in mediator solutions generated large cathodic catalytic currents easily recorded by cyclic voltammetry at lowpotential scan rates. This current showed two well-defined pH profiles, which correlated with the variation of the mediator redox potentials at the pH range tested. The relevant effect of temperature on the activity of laccase has been assessed here. Likewise, it was shown that the current record varied with the substrate concentration. This trend fitted Michaelis -Menten kinetics, which allowed us to give an estimation of the affinity of the fungal laccase for the different mediators. D

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Section: Discussionmentioning

confidence: 99%

Voltammetric monitoring of laccase-catalysed mediated reactions

Fernández‐Sánchez

Tzanov

Gübitz

et al. 2002

Bioelectrochemistry

116

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“…3a). This is a voltammetric response of surface bound species for which there is instant charge compensation by ions from the organic phase [24][25][26][27][28]. It is interesting to notice that if the forward peak potential remains constant, the backward peak potential shifts upon increasing scan rates. )…”

Section: Electrochemical Behaviour Of (Plys/porphyrin) N Multilayersmentioning

confidence: 99%

Porphyrin “Mille-Feuilles” photo-electrodes

Tan

Hojeij

2008

Journal of Electroanalytical Chemistry

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“…The commercial FDH preparation was used as received. Horseradish peroxidase was modified as previously reported by oxidation of the glycosidic residues in order to render an overall negative charge after Schiff base formation with taurine [42]. The modification of AOX, a non glycosilated enzyme, was done by the nucleophilic substitution of the free amino groups of the protein with the halogenated derivative containing a trimethyl-ammonium terminal group.…”

Section: Description Of the Polyelectrolytesmentioning

confidence: 99%

“…It is of general acceptance that layer-by-layer assembly is a powerful approach for integrating functional structures into ultrathin films and on a large variety of materials including planar solid substrates [17,18], micro-and nanometer scale particles [19], nanofibers [20], and nanotubes [21,22]. The assembly of nanomaterials in the so-called hybrid systems has also been reported [23 -26].…”

Section: Introductionmentioning

confidence: 99%

“…Previously, we have reported on the ordered assembly of a polycationic osmium based redox polymer describing the charge transfer mechanism through the different layers. We also reported the implementation of this electrochemical interface for tailoring biosensor characteristics [42]. Herein, we further develop this concept by strategic manipulation of the polyelectrolyte charge resulting in a new electrochemical interface with zwitterionic character and a means to shift the overall charge of catalytic proteins.…”

Section: Introductionmentioning

confidence: 99%

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Electrostatic Assemblies for Bioelectrocatalytic and Bioelectronic Applications

2006

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This work extensively covers the use of layer-by-layer (LbL) assembly of polyelectrolytes for the transduction of catalytic and affinity events. It is demonstrated that by controlling the charge of polyelectrolytes, electrostatic interactions drive then the LbL construction of supramolecular architectures with improved performance. Particularly, by adequately charging an osmium based redox polymer, a multicofactor protein (fructose dehydrogenase) may be deposited in a favored orientation resulting in a more efficient electrochemical communication. A H 2 O 2 transducing interface is created by the enhancement of electrostatic interactions between the electrochemical and catalytic layer. Further assemblies include the coupling of an oxidase (alcohol oxidase) with the H 2 O 2 transducing interface resulting in a linear increase of current with the number of AOX layers. Finally, affinity assemblies are demonstrated by deposition of anti Dexamethasone antibodies. Faraday currents are then obtained by the electrochemical communication between the HRP labeled immunoconjugate and the electrochemical interface in a heterogeneous competitive assay format.

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Reagentless biosensors based on self-deposited redox polyelectrolyte-oxidoreductases architectures

Cited by 103 publications

References 42 publications

Voltammetric monitoring of laccase-catalysed mediated reactions

Voltammetric monitoring of laccase-catalysed mediated reactions

Porphyrin “Mille-Feuilles” photo-electrodes

Electrostatic Assemblies for Bioelectrocatalytic and Bioelectronic Applications

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