Reactivity of Two Gonococcal Antigens in an Automated Microhemagglutination Procedure

Logan, Leslie C.; Cox, Patricia M.; Norins, Leslie C.

doi:10.1128/aem.20.6.907-909.1970

Cited by 8 publications

(6 citation statements)

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“…Study of a passive haemagglutination test for gonorrhoea AND C. S. NICOL In recent years the rising incidence of gonorrhoea in Europe and America has stimulated research in immunological methods for its diagnosis. Most serological techniques have been applied; bacterial agglutination (Wilson, 1954), passive haemolysis (Maeland, 1966), complement-fixation (Magnussen and Kjellander, 1965), passive haemagglutination (Chanarin, 1954;Logan, Cox, and Norins, 1970), latex-agglutination (Watt, Ward, and Glynn, 1971), Bentonite agglutination (Wallace, Diena, Yugi, and Greenberg, 1970), microprecipitation (Reising and Kellogg, 1965;Chacko and Nair, 1969), lecithincholesterol particle agglutination (Reising, 1971), and fluorescent antibody staining methods (Ovcinnikov, 1963;Danielsson, 1965a).…”

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confidence: 99%

“…Some tests have utilized phenol-extracted antigens. Many workers have reported interference in their tests by cross-reacting antibody to other bacterial species (Reyn, 1943(Reyn, , 1949Danielsson, 1965b;Logan, Cox, and Norins, 1970). The use of phenol-extracted antigen in some tests has given rise to fewer cross-reactions, although this is accompanied by a loss of sensitivity.…”

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confidence: 99%

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Study of a passive haemagglutination test for gonorrhoea.

Fletcher¹,

Miller²,

Nicol³

1973

Sexually Transmitted Infections

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confidence: 99%

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confidence: 99%

Study of a passive haemagglutination test for gonorrhoea.

Fletcher¹,

Miller²,

Nicol³

1973

Sexually Transmitted Infections

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“…Before the samples were injected into animals, they were sterilized by passage through a 0.22-Mm membrane filter (Millipore Corp.). Normal or immune sera and fractions were assayed for agglutinins by passive hemagglutination (9). Immunoelectrophoresis was performed according to the method of Scheidegger (14).…”

Section: Materials and Methods Animalsmentioning

confidence: 99%

Development and Passive Transfer of Immunity to Gonococcal Infection in Guinea Pigs

Scales

Kraus

1974

Infect Immun

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Gonococcal infections of relatively long duration were produced in guinea pigs with minimal infective doses ranging from 101 to 102 colony-forming units. After spontaneous eradication and upon rechallenge with > 108 gonococcal colony-forming units, guinea pigs were refractory to infection. Serum from these guinea pigs was bactericidal in vitro and protected virgin guinea pigs from in vivo challenge. The 7S peak, but not the 19S peak, from gel filtered immune serum demonstrated both bactericidal and passive protective properties. In vitro bactericidal activity of whole immune serum and its 7S peak was abolished after heating at 56 C for 30 min. The in vivo protective ability of the 7S peak was not abolished after heating. Previous studies have indicated that some degree of an immune response, both cellular and humoral, develops in man after natural gonococcal infections (5, 7). However, repeated

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“…During the past 10 years, there has been increasing interest in serological methods for the diagnosis of gonococcal infections. A variety of serological techniques have been described, including the complement fixation test (4,26), flocculation (18, 31), direct hemagglutination (9,19,32), and the indirect immunofluorescence test (2,34,35). Recently, a radioimmunoassay (1,21) and an indirect hemagglutination test (25) in which gonococcal pilus antigen is used to detect gonococcal antibodies have been described, but the results of these tests have been variable because of a lack of sufficient sensitivity or specificity.…”

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confidence: 99%

Passive hemagglutination test for detection of antibody to gonococcal ribosomal antigen in sera from patients with asymptomatic gonorrhea

Kita¹,

Kashiba²

1982

J Clin Microbiol

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Ribosomal fractions were obtained from a culture of type 2 Neisseria gonorrhoeae strain P-17 which was isolated from a patient with an acute gonococcal infection; these fractions were purified to eliminate the components of the outer membrane complex by affinity chromatography (Sepharose-anti-outer membrane complex antibody conjugates were used as the solid immunosorbent), and the resulting preparation was designated the purified ribosomal fraction. The purified ribosomal fraction was used to detect antibody activity in sera obtained from culture-positive asymptomatic carriers and healthy controls by a passive hemagglutination test. This passive hemagglutination test had a specificity of 100% for both sexes and sensitivities of 99.4 and 88.2% for female and male carriers, respectively, when an antibody titer of more than 1:3 was defined as abnormal. Absorption of the sera with nongonococcal organisms did not affect the antibody activity, and no significant difference in antigenicity among various N. gonorrhoeae strains was observed in ribosomal fractions. An enzyme-linked immunosorbent assay was also used to measure the relative amounts of specific antibodies to the purified ribosomal fraction, and this assay revealed that the anti-purified ribosomal fraction antibodies were immunoglobulin G.

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Reactivity of Two Gonococcal Antigens in an Automated Microhemagglutination Procedure

Cited by 8 publications

References 1 publication

Study of a passive haemagglutination test for gonorrhoea.

Study of a passive haemagglutination test for gonorrhoea.

Development and Passive Transfer of Immunity to Gonococcal Infection in Guinea Pigs

Passive hemagglutination test for detection of antibody to gonococcal ribosomal antigen in sera from patients with asymptomatic gonorrhea

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