Study of a passive haemagglutination test for gonorrhoea.

Fletcher, S.M.; Miller, R; Nicol, C. S.

doi:10.1136/sti.49.6.508

Cited by 4 publications

(3 citation statements)

References 12 publications

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“…It is tempting to conclude that patients with gonorrhoea are stimulated to produce IgG antibodies against endotoxin of the gonococcus. This is in agreement with reports by other investigators that patients with gonorrhoea produce humoral antibodies in response to the infecting organism (Cohen, Kellogg, and Norins, 1969;Fletcher, Miller, and Nicol, 1973;Danielsson and others, 1972;Wallace, Diena, Yugi, and Greenberg, 1970;Ward and Glynn, 1972). On the other hand, Watt, Ward, and Glynn (1971) have reported results which differ from those of the present study.…”

Section: Discussionsupporting

confidence: 92%

Mercaptoethanol-resistant human serum antibodies reacting with endotoxin from Neisseria gonorrhoeae.

Mæland¹,

Larsen²

1975

Sexually Transmitted Infections

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Section: Discussionsupporting

confidence: 92%

Mercaptoethanol-resistant human serum antibodies reacting with endotoxin from Neisseria gonorrhoeae.

Mæland¹,

Larsen²

1975

Sexually Transmitted Infections

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“…The predictive values of positive and negative test results ( 19) were calculated on the assumption that a positive test result obtained for patients without current gc were false positive, although previous gc had occurred in 29 % female and 45 % male patients (Tables 5 and 6). The number of patients in whom a positive serological test result was unrelated to exposure to gonorrhoea was low, since 8 out of 16 female and 7 (6,20). Neither was it possible to distinguish between recent and past infection by determination of the immunoglobulin class of the antibodies present (10, 16) because the immune response was found to be dominated by IgG antibodies.…”

Section: Discussionmentioning

confidence: 91%

An Indirect Haemagglutination Test for Demonstration of Gonococcal Antibodies Using Gonococcal Pili as Antigen

Reimann

Lind

Andersen

1980

Acta Pathologica Microbiologica Scandinavica Section C Immunolo

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A total of 1223 serum specimens were obtained from 649 consecutive patients attending a dermato‐venereological out‐patient clinic in Copenhagen with a request for venereal disease control. The sera were examined for gonococcal antibodies by both a gonococcal complement fixation test (GCF) and an indirect haemagglutination test using gonococcal pili as antigen (IHA). The diagnosis of current gonococcal infection in 28 per cent of the patients was based on positive culture for Neisseria gonorrhoeae from one or more of the following sites: urethra, rectum and fauces in all patients, and/or cervix in female patients. The specificity, sensitivity and predictive values of positive and negative test results were calculated for the various groups of patients on the assumption that all positive results, both in patients without gonorrhoea but with a previous gonococcal infection and in patients without known current or previous infection, were false positives. The following values were found: Sensitivity: IHA 45–100%, GCF 6–29%. Specificity: IHA 65–89%, GCF 97–100%. Predictive value of positive test result: IHA 43–74%, GCF 69–100%. Predictive value of negative test result: IHA 78–100%, GCF 61–85%.

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“…During the past 10 years, there has been increasing interest in serological methods for the diagnosis of gonococcal infections. A variety of serological techniques have been described, including the complement fixation test (4,26), flocculation (18, 31), direct hemagglutination (9,19,32), and the indirect immunofluorescence test (2,34,35). Recently, a radioimmunoassay (1,21) and an indirect hemagglutination test (25) in which gonococcal pilus antigen is used to detect gonococcal antibodies have been described, but the results of these tests have been variable because of a lack of sufficient sensitivity or specificity.…”

mentioning

confidence: 99%

Passive hemagglutination test for detection of antibody to gonococcal ribosomal antigen in sera from patients with asymptomatic gonorrhea

Kita¹,

Kashiba²

1982

J Clin Microbiol

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Ribosomal fractions were obtained from a culture of type 2 Neisseria gonorrhoeae strain P-17 which was isolated from a patient with an acute gonococcal infection; these fractions were purified to eliminate the components of the outer membrane complex by affinity chromatography (Sepharose-anti-outer membrane complex antibody conjugates were used as the solid immunosorbent), and the resulting preparation was designated the purified ribosomal fraction. The purified ribosomal fraction was used to detect antibody activity in sera obtained from culture-positive asymptomatic carriers and healthy controls by a passive hemagglutination test. This passive hemagglutination test had a specificity of 100% for both sexes and sensitivities of 99.4 and 88.2% for female and male carriers, respectively, when an antibody titer of more than 1:3 was defined as abnormal. Absorption of the sera with nongonococcal organisms did not affect the antibody activity, and no significant difference in antigenicity among various N. gonorrhoeae strains was observed in ribosomal fractions. An enzyme-linked immunosorbent assay was also used to measure the relative amounts of specific antibodies to the purified ribosomal fraction, and this assay revealed that the anti-purified ribosomal fraction antibodies were immunoglobulin G.

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Study of a passive haemagglutination test for gonorrhoea.

Cited by 4 publications

References 12 publications

Mercaptoethanol-resistant human serum antibodies reacting with endotoxin from Neisseria gonorrhoeae.

Mercaptoethanol-resistant human serum antibodies reacting with endotoxin from Neisseria gonorrhoeae.

An Indirect Haemagglutination Test for Demonstration of Gonococcal Antibodies Using Gonococcal Pili as Antigen

Passive hemagglutination test for detection of antibody to gonococcal ribosomal antigen in sera from patients with asymptomatic gonorrhea

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