Reactive oxygen species production and antioxidant enzyme activity during epididymal sperm maturation in Corynorhinus mexicanus bats

Arenas‐Ríos, Edith; Adolfo, Rosado García; Cortés-Barberena, Edith; Königsberg, Mina; Arteaga-Silva, Marcela; Rodríguez-Tobón, Ahiezer; Gisela, Fuentes-Mascorro; Ángel, León-Galván Miguel

doi:10.1016/j.repbio.2016.01.001

Cited by 6 publications

(6 citation statements)

References 21 publications

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“…GSH-Px is an important enzyme in the body that catalyzes the decomposition of hydrogen peroxide, and it is also an important antioxidant and free radical scavenger in the body. GSH-Px specifically catalyze the reduction response of glutathione to hydrogen peroxide, which can protect the integrity of cell membranes and functions [18, 19]. SOD, an important antioxidant enzyme in the body, is a natural free radical scavenging factor in the body, which can transform harmful free radicals into hydrogen peroxide, and its vitality indirectly reflects the body’s free radical scavenging ability.…”

Section: Discussionmentioning

confidence: 99%

Effect of Polygonum Multiflorum Thunb on liver fatty acid content in aging mice induced by D-galactose

Yang

Zou

et al. 2019

Lipids Health Dis

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Background Polygonum Multiflorum Thunb (PMT) has multiple biological effects, such as anti-inflammatory, lipid-lowering, anti-aging and so on. Therefore, D-galactose-induced aging mice were used to study the effect of PMT on fatty acid metabolism and its underlying mechanism. Methods C57BL/6 male mice were randomly divided into normal group, aging model group, PMT intragastrical administration group (high, Medium, low); model group and PMT intragastrical administration group Daily intraperitoneal injection D-galactose 800 mg·ml − 1 ·Kg − 1 to establish subacute aging model; PMT intragastrical administration group at the same time to intragastrical PMT extract (1 g·ml − 1 ·Kg − 1 , 0.6 g·ml − 1 ·Kg − 1 , 0.3 g·ml − 1 ·Kg − 1 ), normal group injection and intragastrical equivalent saline for 60 consecutive days. By detecting the oxidation index of liver to judge the efficacy of PMT, gas chromatography-mass spectrometry (GC-MS) analysis was used to quantitatively analyze the fatty acid content in liver. Results Finally, we found that PMT improved the enzyme activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in aging mice, and reduce the enzyme activity of malondialdehyde (MDA), aspartate aminotransferase (AST) and alanine aminotransferase (ALT). The content of fatty acids such as C18:1, C18:2, C18:3 N3, C20:2 and C20:3 N3 decreased significantly in senescent mice ( P < 0.05) as evidenced by GC-MS analysis, whereas, these fatty acids increased significantly after treatment of PMT ( P < 0.05). Conclusion PMT improves the content of liver fatty acids in aging mice induced by D-galactose through, enhancing the activity of anti-oxidant enzymes.

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Section: Discussionmentioning

confidence: 99%

Effect of Polygonum Multiflorum Thunb on liver fatty acid content in aging mice induced by D-galactose

Yang

Zou

et al. 2019

Lipids Health Dis

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“…The homogenates obtained were centrifuged for 10 min at 5000 × g. The precipitates were discarded, and all enzymatic activity was measured in the supernatants. The assays to quantify enzymatic activity were done as described previously [ 15 ]. GPX (EC.I.II.I.9) activity was determined at 25°C using Ransel kits (RS504, Randox Laboratories Limited, Crumlin, Northern Ireland) to determine NADPH oxidation at 340 nm for 5 min [ 51 ].…”

Section: Methodsmentioning

confidence: 99%

“…Total protein content was measured using a commercially available bi-cinchoninic acid protein assay (23225, Pierce Thermo Scientific, Rockford, IL, USA). All assays were run in duplicate using single and double amounts of homogenate (6 testicles and 6 epididymides were used for the homogenate) [ 15 ].…”

Section: Methodsmentioning

confidence: 99%

Section: Antioxidant Enzyme Activitymentioning

confidence: 99%

“…Sperm are at serious risk from the moment they form in the testicles and during transit through the epididymides, since the processes in which they participate are highly sensitive to ROS, especially superoxide and hydrogen peroxide [ 15 ], substances that can cause membrane damage and DNA fragmentation in sperm.…”

Section: Introductionmentioning

confidence: 99%

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Sperm Dysfunction in the Testes and Epididymides due to Overweight and Obesity Is Not Caused by Oxidative Stress

Ruiz-Valderrama

Posadas-Rodríguez

Bonilla-Jaime

et al. 2022

International Journal of Endocrinology

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Obesity is a condition that has been linked to male infertility. The current hypothesis regarding the cause of infertility is that sperm are highly sensitive to reactive oxygen species (ROS) during spermatogenesis in the testes and transit through the epididymides, so the increase in ROS brought on by obesity could cause oxidative stress. The aim of this study was to evaluate whether the activity of the enzymes catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPX) is capable of counteracting oxidative stress in sperm. The male Wistar rat was used as an overweight and obesity model, and analysis of fertility in these groups was carried out including the control group. Serum testosterone levels were determined, and the scrotal fat, testes, and epididymides were extracted. The epididymides were separated ini0 3 principal parts (caput, corpus, and cauda) before evaluating sperm viability, sperm morphology, damage to desoxyribonucleic acid of the sperm, and ROS production. The protein content and specific activity of the three enzymes mentioned above were evaluated. Results showed a gain in body weight and scrotal fat in the overweight and obese groups with decreased parameters for serum testosterone levels and sperm viability and morphology. Fertility was not greatly affected and no DNA integrity damage was found, although ROS in the epididymal sperm increased markedly and Raman spectroscopy showed a disulfide bridge collapse associated with DNA. The specific activities of CAT and GPX increased in the overweight and obesity groups, but those of SOD did not change. The amounts of proteins in the testes and epididymides decreased. These findings confirm that overweight and obesity decrease concentrations of free testosterone and seem to decrease protein content, causing poor sperm quality. Implications. An increase in scrotal fat in these conditions fosters an increase of ROS, but the increase of GPX and CAT activity seems to avoid oxidative stress increase in the sperm without damaging your DNA.

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Alternative reproductive tactics, sperm mobility and oxidative stress in Carollia perspicillata (Seba’s short-tailed bat)

Fasel

Wesseling

Fernandez

et al. 2016

Behav Ecol Sociobiol

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In social systems with alternative reproductive tactics, sneakers face a higher level of sperm competition than harem males and hence are predicted to allocate more resources to ejaculates. Antioxidants can protect sperm against oxidative stress, and thus, their allocation to the ejaculate may depend on mating tactic. In this study on the frugivorous bat Carollia perspicillata, we assessed, for harem and sneaker males, four sperm mobility traits, blood and ejaculate markers of the redox balance and the ejaculate to blood ratios of the redox markers. Under higher sperm competition, sneaker males should allocate proportionally more antioxidant resources to the protection of sperm than harem males. In contrast, harem males should favour pre-copulatory functions, which comprise the protection of blood. We found significantly higher sperm velocity and sperm survival in sneakers. There was no correlation between sperm mobility and sperm enzymatic antioxidant activity or ejaculate levels of lipid peroxidation (oxidative damage). Ejaculate levels of lipid peroxidation and sperm survival showed a significantly positive correlation, which could be attributed to the role of reactive oxygen species for sperm capacitation. Harem and sneaker males showed similar levels of redox balance markers in ejaculate and blood. However, harem males showed a higher ratio of oxidized over reduced glutathione in blood, which may indicate higher cellular stress due to higher metabolism. Overall, our findings suggest that sneakers of C. perspicillata compensate for a higher level of sperm competition by higher sperm mobility. Significance statementIn social systems with alternative reproductive tactics, sneakers face higher level of sperm competition than harem males and hence are predicted to allocate more resources to ejaculates. Antioxidants can protect sperm against oxidative stress, and thus, their allocation to the ejaculate may depend on mating tactic. In this study on the frugivorous bat Carollia perspicillata, we found sperm swimming significantly faster and longer in sneaker males compared to harem males. However, traits other than the investigated antioxidant may favour higher sperm mobility. Measured redox pattern in blood of harem males may indicate higher cellular stress due to higher metabolism. Our results provide support to the current sperm competition models at the intraspecific level, which is still debated for internal fertilizers. This study contributes to better understanding the trade-offs and adaptations resulting from alternative reproductive tactics in mammals.

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Reactive oxygen species production and antioxidant enzyme activity during epididymal sperm maturation in Corynorhinus mexicanus bats

Cited by 6 publications

References 21 publications

Effect of Polygonum Multiflorum Thunb on liver fatty acid content in aging mice induced by D-galactose

Effect of Polygonum Multiflorum Thunb on liver fatty acid content in aging mice induced by D-galactose

Sperm Dysfunction in the Testes and Epididymides due to Overweight and Obesity Is Not Caused by Oxidative Stress

Alternative reproductive tactics, sperm mobility and oxidative stress in Carollia perspicillata (Seba’s short-tailed bat)

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