Reactive Oxygen Species, Cell Growth, and Taxol Production of <I>Taxus cuspidata</I> Cells Immobilized on Polyurethane Foam

Abstract

Dynamic changes in reactive oxygen species (ROS) of Taxus cuspidata cells immobilized on polyurethane foam were investigated and the relation between ROS content and taxol production was discussed. Immobilization shortened the lag period of cell growth and moderately increased H2O2 and O2-* contents inside the microenvironment within the first 15 d. After 20 d, excessive production of H2O2 and O2-* was observed accompanied by marked increases in membrane lipid peroxidation and cell membrane permeability. The taxol content of immobilized cells was fourfold that of suspended cells at d 35. The addition of exogenous H2O2 barely affected malondialdehyde content and cell membrane permeability but led to an obvious accumulation of taxol. It is inferred that the intracellular and extracellular H2O2 inside the microenvironment might be one factor promoting taxol biosynthesis under the immobilization stress.

Spatial–temporal distribution of nitric oxide involved in regulation of phenylalanine ammonialyase activation and Taxol production in immobilized Taxus cuspidata cells

“…Meanwhile the microenvironments were influenced by multiple factors, such as restriction by reticulated foam, nutriment transportation and oxygen diffusion (Yin et al, 2005), so the endogenous NO was produced differently in different layers in a long term. Fig.…”

“…The fungal elicitor and ultrasound (Wu and Lin, 2003) improved the Taxol production accompanied with increase of PAL activity (Yuan et al, 2001;Dixon and Paiva, 1995). As PAL was also the key enzyme in forming the side chain during Taxol biosynthesis, the increased PAL activity promoted Taxol production in immobilized T. cuspidata cells (Yin et al, 2005). Walker first illustrated that a phenylalanine aminomutase with high similarity to PAL was involved in taxane side chain synthesis (Walker and Floss, 1998).…”

“…Immobilization by polyurethane foam provided cells with a peculiar spatial microenvironment where cells were regulated by reticulated foam, nutriment transportation and oxygen diffusion, obviously affecting cell growth, secondary metabolites production and phenylalanine ammonialyase (PAL) expression (Yin et al, 2005). Previous results revealed that extracellular signal-regulated kinase-like (ERK-like) mitogen-activated protein kinase (MAPKs) and arabinogalactan proteins were involved in cellular proliferation, differentiation, and Taxol production of immobilized T. cuspidata cells (Cheng et al, 2006(Cheng et al, , 2008.…”

Increased paclitaxel recovery from Taxus baccata vascular stem cells using novel in situ product recovery approaches

“…This specific combination was selected as it has been shown to be effective in the recovery of taxanes from yeast cell factories (Santoyo-Garcia et al 2023 ). As demonstrated in previous studies, the total concentration of the resins in the culturable cells is a critical variable for cell growth (Yin et al 2005 ; Santoyo-Garcia et al 2022 ). For that reason, treatment A configuration (0.5, 1 and 1.5% w/v of HP-20, XAD7 and HP-2MG, respectively) of resins at different total concentrations (1.5, 3 and 6% w / v ) was utilised to test cell growth and taxanes production in VSCs.…”

Effect of dihydrotestosterone on mouse embryonic stem cells exposed to H2O2-induced oxidative stress

“…The major oxygen species responsible for these oxidative stresses are hydrogen peroxide (H 2 O 2 ), the free radical superoxide anion (O 2- ) and the hydroxyl radical (OH - ) [6]. Among the ROS, H 2 O 2 has been implicated as a cellular toxin [19,61]. Since H 2 O 2 has remarkable membrane permeability [23], intracellular H 2 O 2 can induce detrimental effects in cells [20,41,42,49].…”