Spatial–temporal distribution of nitric oxide involved in regulation of phenylalanine ammonialyase activation and Taxol production in immobilized Taxus cuspidata cells

Xiao, Wenhai; Cheng, Jun; Yuan, Ying‐Jin

doi:10.1016/j.jbiotec.2008.11.005

Cited by 10 publications

(6 citation statements)

References 37 publications

(45 reference statements)

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“…PcPAL2 and PcPAL3) site-directly modified at the Cys557 probably will clarify this initial observation. Interestingly, NO fumigation or NO donor treatment is often associated with a rapid increase in the activity of PAL concomitant with an accumulation of phenolic and flavonoid compounds [118]–[120]. In our work, COMT became significantly de-nitrosylated upon ozone fumigation.…”

Section: Discussionsupporting

confidence: 45%

S-Nitroso-Proteome in Poplar Leaves in Response to Acute Ozone Stress

et al. 2014

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Protein S-nitrosylation, the covalent binding of nitric oxide (NO) to protein cysteine residues, is one of the main mechanisms of NO signaling in plant and animal cells. Using a combination of the biotin switch assay and label-free LC-MS/MS analysis, we revealed the S-nitroso-proteome of the woody model plant Populus x canescens. Under normal conditions, constitutively S-nitrosylated proteins in poplar leaves and calli comprise all aspects of primary and secondary metabolism. Acute ozone fumigation was applied to elicit ROS-mediated changes of the S-nitroso-proteome. This treatment changed the total nitrite and nitrosothiol contents of poplar leaves and affected the homeostasis of 32 S-nitrosylated proteins. Multivariate data analysis revealed that ozone exposure negatively affected the S-nitrosylation status of leaf proteins: 23 proteins were de-nitrosylated and 9 proteins had increased S-nitrosylation content compared to the control. Phenylalanine ammonia-lyase 2 (log2[ozone/control] = −3.6) and caffeic acid O-methyltransferase (−3.4), key enzymes catalyzing important steps in the phenylpropanoid and subsequent lignin biosynthetic pathways, respectively, were de-nitrosylated upon ozone stress. Measuring the in vivo and in vitro phenylalanine ammonia-lyase activity indicated that the increase of the phenylalanine ammonia-lyase activity in response to acute ozone is partly regulated by de-nitrosylation, which might favor a higher metabolic flux through the phenylpropanoid pathway within minutes after ozone exposure.

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Section: Discussionsupporting

confidence: 45%

S-Nitroso-Proteome in Poplar Leaves in Response to Acute Ozone Stress

et al. 2014

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“…in vitro cultures has been confirmed Wu 2004, 2005;Xu et al 2004;Wang et al 2006;Xiao et al 2009). Although PAL does not participate in the formation of the paclitaxel C-13 side chain, several inhibitors of this enzyme were found to stop paclitaxel production almost entirely (Brincat et al 2002).…”

Section: Introductionmentioning

confidence: 82%

“…in vitro cultures. Xiao et al (2009) investigated the production and distribution of NO in immobilized suspension cultures of T. cuspidata. The highest paclitaxel content coincided with the highest NO accumulation in cell aggregates.…”

Section: Taxane Production and Pal Activitymentioning

confidence: 99%

Paclitaxel production and PAL activity in hairy root cultures of Taxus x media var. Hicksii carrying a taxadiene synthase transgene elicited with nitric oxide and methyl jasmonate

et al. 2015

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The role of sodium nitroprusside (SNP, 10 lM), a nitric oxide (NO) donor, and/or methyl jasmonate (MJ, 100 lM) spiked with L-phenylalanine (PHEN, 100 lM) and additional sucrose (S; 30 g l -1 ), in taxane production and phenyl ammonia lyase (PAL) activity in cultures of two Taxus media x var. Hicksii transgenic root lines (ATMA and ATM) carrying the taxadiene synthase transgene was investigated. SNP addition, when applied together with MJ and/or PHEN, resulted in paclitaxel production only in ATMA cultures. The application of the NO donor gave the highest paclitaxel content (7.56 mg l -1) in the combination of SNP?S?MJ?PHEN, after 2 weeks of treatment in the ATMA root line. In ATM cultures, taxane production was not affected by SNP. In both ATMA and ATM lines the highest total (intra?extracellular) paclitaxel yield was determined when elicited with MJ?PHEN, and amounted to 10.78 mg l -1 at 1 week and 1.63 mg l -1 at 2 weeks of treatment, in cultures of ATMA and ATM lines, respectively. The excretion of paclitaxel was observed only in ATMA cultures, with the highest level (2.34 mg l -1 ) obtained after elicitation with S?MJ?PHEN. The comparison of PAL activity in the two root lines revealed that this enzyme was almost 3-times more active in ATM than ATMA roots. An increase in both PAL activity and paclitaxel production was only observed in ATMA cultures growing in medium supplemented with S?MJ?PHEN.

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“…(5) In Hibiscus moscheutos , root elongation is markedly reduced by treatment with l ‐NNA (Tian et al., 2007). (6) In Taxus cuspidata cells, NO production is substantially reduced after incubation with l ‐NNA (Xiao et al ., 2009). (7) In Salvia miltiorrhiza hairy roots, ATP‐induced NO production is significantly suppressed by l ‐NAME (Wu & Wu, 2008).…”

Section: Pharmacological and Indirect Genetic Studies Also Support Thmentioning

confidence: 99%

Evidence supporting the existence of l‐arginine‐dependent nitric oxide synthase activity in plants

et al. 2009

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Spatial–temporal distribution of nitric oxide involved in regulation of phenylalanine ammonialyase activation and Taxol production in immobilized Taxus cuspidata cells

Cited by 10 publications

References 37 publications

S-Nitroso-Proteome in Poplar Leaves in Response to Acute Ozone Stress

S-Nitroso-Proteome in Poplar Leaves in Response to Acute Ozone Stress

Paclitaxel production and PAL activity in hairy root cultures of Taxus x media var. Hicksii carrying a taxadiene synthase transgene elicited with nitric oxide and methyl jasmonate

Evidence supporting the existence of l‐arginine‐dependent nitric oxide synthase activity in plants

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