Reactive oxygen species and antioxidant system responses in wheat cultivars during interaction with Fusarium species

Khaledi, Nima; Taheri, Parissa; Falahati-Rastegar, Mahrokh

doi:10.1007/s13313-016-0455-y

Cited by 27 publications

(37 citation statements)

References 85 publications

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“…Such a response might be connected to the formation of the natural barrier against Fusarium penetration at the beginning of the spore infestation. A similar result was found in FHB-partially resistant wheat genotype "Gaskozhen", which responded to Fusarium graminearum and F. culmorum by enhanced production of H 2 O 2 and high induction of POD [13]. On the other hand, induced POD activity detected in "Lucija" after Fusarium attack could be the result of the "oxidative burst".…”

Section: Discussionsupporting

confidence: 75%

“…Polyphenol oxidase (PPO) as a part of non-enzymatic antioxidative mechanism is involved in a defence response provoked by pathogen [18]. Induced activity of the antioxidative system together with the non-enzymatic pathways appeared to be an important indicator for disease resistance [13,25]. In contrast, ROS production can be beneficial for the plant immunity.…”

Section: Introductionmentioning

confidence: 99%

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Biochemical changes in ears of wheat genotypes subjected to Fusarium spp. attack

Marček

Vuletić

Španić

2018

Acta Biologica Hungarica

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In wheat, Fusarium fungus promotes the appearance of destructive disease named as Fusarium head blight (FHB) that can cause grain yield reduction and mycotoxin accumulation. The focus of this research was to verify the influence of Fusarium graminearum and F. culmorum on wheat genotypes with different susceptibility to FHB: "Super Žitarka" (susceptible), "Lucija" (moderately resistant) and "Apache" (resistant). The experiment was performed under field conditions by artificial spore inoculation of ears at the flowering stage. The effectiveness of antioxidative enzymes, hydrogen peroxide (H 2 O 2) content and malondialdehyde (MDA) content were observed at several sampling points after Fusarium inoculation (3, 15 and 24 hours). "Lucija" responded to pathogen by increase of guaiacol peroxidase (POD) activity, high H 2 O 2 and MDA content in the early post-inoculation times (3 and 15 hours), compared to control. "Super Žitarka" displayed inhibition of catalase (CAT) activity throughout the whole time course of the experiment. Infected plants of "Apache" showed notable decline in MDA content over time. Moreover, in "Apache" increased H 2 O 2 accumulation was observed immediately after Fusarium exposure (3 and 15 hours), compared to 24 hours. Rapid overproduction of H 2 O 2 under Fusarium stress marked "Apache" as FHB-resistant.

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Section: Discussionsupporting

confidence: 75%

Section: Introductionmentioning

confidence: 99%

Biochemical changes in ears of wheat genotypes subjected to Fusarium spp. attack

Marček

Vuletić

Španić

2018

Acta Biologica Hungarica

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“…The Iranian wheat cultivars with different genetic backgrounds showed different levels of resistance to FHB. A difference in defense responses under FHB inoculation was observed in partially resistant (Gaskozhen) and susceptible (Falat) cultivars, which agrees with Khaledi et al (2016).…”

Section: Considerably Higher Ohsupporting

confidence: 81%

“…After recognition of the pathogen, basal defense responses lead to activation of several resistance mechanisms such as production of reactive oxygen species (ROS) (Shetty et al 2008;Khaledi et al 2016), reactive nitrogen species (RNS) (Hong et al 2008;Duan et al 2015), deposition of callose (Ellinger et al 2014), enzymatic and non-enzymatic antioxidants (Zhou et al 2007; Khaledi et al 2016). In plant-pathogen interactions, one of the earliest plant defense responses is production of ROS (Shetty et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of resistance and the role of some defense responses in wheat cultivars to Fusarium head blight

Khaledi¹,

Taheri²,

Falahati-Rastegar³

2018

Journal of Plant Protection Research

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Fusarium graminearum and F. culmorum are the causal agents of Fusarium head blight (FHB) in cereal crops worldwide. Application of resistant cultivars is the most effective and economic method for management of FHB and reducing mycotoxin production in wheat. Understanding the physiological and biochemical mechanisms involved in basal resistance of wheat to FHB disease is limited. In this research, after screening resistance levels of eighteen wheat cultivars planted in Iran, Gaskozhen and Falat were identified as partially resistant and susceptible wheat cultivars against Fusarium spp., respectively. Also, we investigated the role of hydroxyl radical (OH − ), nitric oxide (NO), callose deposition, lipid peroxidation and protein content in basal resistance of wheat to the hemi-biotrophic and necrotrophic Fusarium species causing FHB. Nitric oxide as a signaling molecule may be involved in physiological and defensive processes in plants. Our results showed that NO generation increased in seedlings and spikes of wheat cultivars after inoculation with Fusarium species. We observed earlier and stronger callose deposition at early time points after infection by Fusarium spp. isolates than in non-infected plants, which was positively related to the resistance levels in wheat cultivars. Higher levels of OH − and malondialdehyde (MDA) accumulation (as a marker of lipid peroxidation) were observed in the Falat than in the Gaskozhen cultivar, under non-infected and infected conditions. So, estimation of lipid peroxidation could be useful to evaluate cultivars' susceptibility. These findings can provide novel insights for better recognition of physiological and biochemical markers of FHB resistance, which could be used for rapid screening of resistance levels in wheat cultivars against this destructive fungal disease.

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“…Plant resistance to Fusarium and mycotoxin production is a highly complex mechanism, including also antioxidant secondary metabolites, which can be toxic to the fungi, participate in cell wall reinforcement as well as interfere with toxin biosynthesis (Atanasova-Penichon et al 2016). Fast activation of the antioxidant defence system was also matched with better resistance levels of plants (Khaledi et al 2016, Spanic et al 2017. Plant growth at elevated CO2 was, however, reported to result in a low level of the antioxidant system in wheat (with very low activities of glutathione reductase, glutathione-S-transferase, guaiacol peroxidase and ascorbate peroxidase, Bencze et al 2014).…”

Section: Don and Zen Concentrations Of The Grains In Response To Elevmentioning

confidence: 99%

Rising atmospheric CO2 concentration may imply higher risk of Fusarium mycotoxin contamination of wheat grains

et al. 2017

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Increasing atmospheric CO2 concentration not only has a direct impact on plants but also affects plant-pathogen interactions. Due to economic and health-related problems, special concern was given thus in the present work to the effect of elevated CO2 (750 μmol mol -1 ) level on the Fusarium culmorum infection and mycotoxin contamination of wheat. Despite the fact that disease severity was found to be not or little affected by elevated CO2 in most varieties, as the spread of Fusarium increased only in one variety, spike grain number and/or grain weight decreased significantly at elevated CO2 in all the varieties indicating that Fusarium infection generally had a more dramatic impact on the grain yield at elevated CO2 than at the ambient level. Likewise, grain deoxynivalenol (DON) content was usually considerably higher at elevated CO2 than at ambient level in the single-floret inoculation treatment, suggesting that the toxin content is not in direct relation to the level of Fusarium infection. In the whole-spike inoculation, DON production did not change, decreased or increased depending on the variety × experiment interaction. Cooler (18°C) conditions delayed rachis penetration while 20°C maximum temperature caused striking increases in the mycotoxin contents, resulting in extreme high DON values and also in a dramatic triggering of the grain zearalenone contamination at elevated CO2. The results indicate that future environmental conditions, such as rising CO2 levels, may increase the threat of grain mycotoxin contamination.Keywords: deoxynivalenol, elevated CO2, Fusarium culmorum, mycotoxin, Triticum aestivum, zearalenone List of abbreviations:AUDPC= area under the disease progress curve DON= deoxynivalenol FHB= Fusarium head blight SFI = single-floret inoculation ZEN = zearalenone WSI= whole-spike inoculation 3

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Reactive oxygen species and antioxidant system responses in wheat cultivars during interaction with Fusarium species

Cited by 27 publications

References 85 publications

Biochemical changes in ears of wheat genotypes subjected to Fusarium spp. attack

Biochemical changes in ears of wheat genotypes subjected to Fusarium spp. attack

Evaluation of resistance and the role of some defense responses in wheat cultivars to Fusarium head blight

Rising atmospheric CO2 concentration may imply higher risk of Fusarium mycotoxin contamination of wheat grains

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