Reactive oxygen intermediates modulate nitric oxide signaling in the plant hypersensitive disease-resistance response

Delledonne, Massimo; Murgia, Irene; Ederle, Davide; Sbicego, Pier Filippo; Biondani, Andrea; Polverari, Annalisa; Lamb, Chris

doi:10.1016/s0981-9428(02)01397-9

Cited by 90 publications

(77 citation statements)

References 41 publications

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“…Although the question as to how NO synthesis is exactly stimulated still stays open, there is plenty of evidence for a complex interplay between ROS and NO. In a balanced model attempting to explain that interplay, Delledone and co-workers propose that ROS and NO act together to determine cell survival or death [34]. Figure 5 summarizes the results presented here and proposes a model of action of the AAL toxin.…”

Section: Distinct Stages Of the Cell Death Processmentioning

confidence: 81%

An extensive microarray analysis of AAL-toxin-induced cell death in Arabidopsis thaliana brings new insights into the complexity of programmed cell death in plants

Gechev

Gadjev

Hille

2004

Cellular and Molecular Life Sciences (CMLS)

129

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An extensive microarray analysis of AAL-toxin-induced cell death in Arabidopsis thaliana brings new insights into the complexity of programmed cell death in plants Gechev, T.S.; Gadjev, I.Z.; Hille, J. Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim. Abstract. A T-DNA knockout of the Arabidopsis homologue of the tomato disease resistance gene Asc was obtained. The asc gene renders plants sensitive to programmed cell death (PCD) triggered by the fungal AAL toxin. To obtain more insights into the nature of AALtoxin-induced cell death and to identify genes of potential importance for PCD, we carried out transcription profiling of AAL-toxin-induced cell death in this knockout with an oligonucleotide array representing 21,500 Arabidopsis genes. Genes responsive to reactive oxygen species (ROS) and ethylene were among the earliest to be upregulated, suggesting that an oxidative burst and production of ethylene played a role in the activation of the cell death. This notion was corroborated by induction of several genes encoding ROS-generating proteins, including a respiratory burst oxidase and germin oxalate oxi- CMLS, Cell. Mol. Life Sci. 61 (2004) 1185 -1197 1420-682X/04/101185-13 DOI 10.1007/s00018-004-4067-2 © Birkhäuser Verlag, Basel, 2004 CMLS Cellular and Molecular Life Sciences dase. Cytochemical studies confirmed the oxidative burst and, in addition, showed synthesis of callose, a feature of the hypersensitive response. A diverse group of transcription factors was also induced. These events were followed by repression of most of the auxin-regulated genes known to be involved in growth and developmental responses. All photosynthesis-related genes were repressed. Blocking the synthesis of ethylene or NO significantly compromised cell death. In addition, we identified a heterogeneous group of early-induced genes, some of them never before associated with PCD. The group of earlyinduced genes included a number of proteases that were previously implicated in developmentally regulated types of PCD, suggesting a more principal role for these proteases in the PCD process. These findings provide new insights into the molecular mechanisms of plant PCD.

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Section: Distinct Stages Of the Cell Death Processmentioning

confidence: 81%

An extensive microarray analysis of AAL-toxin-induced cell death in Arabidopsis thaliana brings new insights into the complexity of programmed cell death in plants

Gechev

Gadjev

Hille

2004

Cellular and Molecular Life Sciences (CMLS)

129

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“…Thus, ethylene and H 2 O 2 may act together to promote cell death, perhaps reinforcing each other. ROS generation can, depending on the dosage, promote a local programmed cell death (32) and induce defense gene expression (7,42). Cell death could be restricted by expression of genes coding for H 2 O 2 -scavenging functions; for instance, a peroxidase-encoding gene is induced in cells surrounding infection pockets (43).…”

Section: Discussionmentioning

confidence: 99%

“…7), also intervene in nodulation. Ethylene plays mostly a negative role in legume symbiosis, probably by restricting infection.…”

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Reactive oxygen species and ethylene play a positive role in lateral root base nodulation of a semiaquatic legume

D’Haeze

Rycke

Mathis

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

172

128

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Lateral root base nodulation on the tropical, semiaquatic legume Sesbania rostrata results from two coordinated, Nod factor-dependent processes: formation of intercellular infection pockets and induction of cell division. Infection pocket formation is associated with cell death and production of hydrogen peroxide. Pharmacological experiments showed that ethylene and reactive oxygen species mediate Nod factor responses and are required for nodule initiation, whereby induction of division and infection could not be uncoupled. Application of purified Nod factors triggered cell division, and both Nod factors and ethylene induced cavities and cell death features in the root cortex. Thus, in S. rostrata, ethylene and reactive oxygen species act downstream from the Nod factors in pathways that lead to formation of infection pockets and initiation of nodule primordia.

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“…Esses comportamentos opostos podem estar relacionados às diferentes doses testadas. O NO atua sinergisticamente com espécies reativas de oxigênio na indução de respostas de defesa em plantas e alterações no equilíbrio entre essas moléculas poderiam explicar, em parte, tanto o efeito tóxico quanto de proteção dessa molécula em suspensões celulares (Delledonne et al 2001(Delledonne et al , 2002.…”

Section: Resultsunclassified

Substâncias antifúngicas constitutivas e induzidas em folhas e suspensões celulares de Rudgea jasminoides (Cham.) Müll. Arg. (Rubiaceae)

2009

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-(Constitutive and induced antifungal compounds in leaves and cell suspension cultures of Rudgea jasminoides (Cham.) Müll. Arg. (Rubiaceae)). Rudgea jasminoides (Cham.) Müll. Arg. is a woody Rubiaceae species from tropical forest that synthesized antifungal secondary metabolites when challenged by fungi. Members of the Rubiaceae are among the few plants that are easily taken into cell cultures to produce substantial amounts of secondary products that surpass those of the whole plant. In this work we analyzed the presence of constitutive and induced antifungal compounds produced by leaves and cell suspension cultures of this species. Oligogalacturonides (OGAs), salicylic acid (AS), nitric oxide (NO), and yeast β-glucan fragments were used as elicitors. Constitutive compounds with antifungal activity were detected in cell suspension cultures of R. jasminoides, mainly during the stationary growth phase. They were found in higher diversity and amounts than those present in leaves. Treatment with β-glucan fragments increased the production of constitutive compounds and induced the synthesis of antifungal metabolites absent in non-elicited cultures (phytoalexins), mainly during the exponential growth phase. OGAs derived from R. jasminoides leaf cell walls showed similar effect. Under our experimental conditions, the endogenous signaling molecules salicylic acid and nitric oxide were toxic to the cells and did not act as elicitors of antifungal compounds in suspension cultures of R. jasminoides. Our data indicate that cell suspension cultures of R. jasminoides are able to synthesize antifungal compounds and their production can be enhanced by fungal or plant elicitors.Key words -antifungal compounds, cell suspension, elicitors, phytoalexin, Rubiaceae RESUMO -(Substâncias antifúngicas constitutivas e induzidas em folhas e suspensões celulares de Rudgea jasminoides (Cham.) Müll. Arg. (Rubiaceae)). Rudgea jasminoides (Cham.) Müll. Arg. é uma rubiácea arbórea nativa da fl oresta tropical que sintetiza metabólitos secundários antifúngicos, quando em contato com fungos. Membros da família Rubiaceae estão entre as poucas plantas facilmente mantidas em cultura de tecidos e que produzem quantidades substanciais de metabólitos secundários que ultrapassam os níveis normalmente encontrados na planta intacta. Neste trabalho foi analisada a presença de compostos antifúngicos constitutivos e induzidos produzidos por folhas e suspensões celulares dessa espécie. Oligogalacturonídeos (OGAs), ácido salicílico(AS), óxido nítrico(NO) e fragmentos de β-glucano de leveduras foram usados como eliciadores. Substâncias constitutivas com atividade antifúngica foram detectadas nas suspensões celulares de R. jasminoides, principalmente na fase estacionária do crescimento das culturas. Essas substâncias foram encontradas em maior diversidade e quantidade quando comparadas às das folhas. O tratamento com fragmentos de β-glucano de leveduras aumentou a produção de substâncias antifúngicas constitutivas e induziu a síntese de compostos antifú...

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Reactive oxygen intermediates modulate nitric oxide signaling in the plant hypersensitive disease-resistance response

Cited by 90 publications

References 41 publications

An extensive microarray analysis of AAL-toxin-induced cell death in Arabidopsis thaliana brings new insights into the complexity of programmed cell death in plants

An extensive microarray analysis of AAL-toxin-induced cell death in Arabidopsis thaliana brings new insights into the complexity of programmed cell death in plants

Reactive oxygen species and ethylene play a positive role in lateral root base nodulation of a semiaquatic legume

Substâncias antifúngicas constitutivas e induzidas em folhas e suspensões celulares de Rudgea jasminoides (Cham.) Müll. Arg. (Rubiaceae)

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