An extensive microarray analysis of AAL-toxin-induced cell death in Arabidopsis thaliana brings new insights into the complexity of programmed cell death in plants

Gechev, Tsanko; Gadjev, Ilya; Hille, Jacques

doi:10.1007/s00018-004-4067-2

Cited by 129 publications

(39 citation statements)

References 51 publications

(66 reference statements)

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“…These lesions are reminiscent of the HR lesions (a type of programmed cell death) that are induced by plant-pathogen interactions and other abiotic stress factors. This type of necrotic lesions was also observed when Arabidopsis was treated with other mycotoxins such as Fumonisin B1 (Stone et al [2000]) and AAL-toxin (Gechev et al [2004]). After infiltration of tomato leaves with FA, symptoms of cell death were observed by Evans blue staining (Figure 1) and also by measuring amount of enhanced ROS levels (Figure 4A, B) as well as by DNA degradation (Figure 8).…”

Section: Discussionmentioning

confidence: 70%

Fusaric acid induced cell death and changes in oxidative metabolism of Solanum lycopersicum L

Singh

Upadhyay

2014

Bot Stud

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BackgroundFusaric acid (FA) has been shown to stimulate the rapid development of disease symptoms, such as necrosis and foliar desiccation. In this study, we have evaluated the phytotoxicity of FA on tomato plants (Solanum lycopersicum L.). FA induced necrotic lesions in detached leaves, which are reminiscent of hypersensitive response (HR) lesions induced by plant-pathogen interactions and other abiotic stress factors.ResultsFA-treated tomato leaves exhibited visible necrotic lesion as a result of cell death which was evident by Evans blue staining, enhanced reactive oxygen species (ROS) levels and DNA degradation. Changes in the generation of O2.- and H2O2 as well as the activities of superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX) were examined in FA-treated tomato leaves. It was observed that FA exposure stimulated oxidative burst in the leaves, resulting in a lasting activation of O2.- and H2O2 production. After first day of FA application, the H2O2 scavenging enzymes CAT and APX showed a strong activity decrease followed by gradual recovery to the control level after 2 and 3 days.ConclusionA concomitant increase in ROS production, the down regulation of antioxidative enzymes activities and upregulation of lipid peroxidation were crucial for the onset of cell death. These results suggested that FA-induced damage might result from ROS pathways. Thus, our experiments provide a useful model plant system for research on FA-induced plant cell death.Electronic supplementary materialThe online version of this article (doi:10.1186/s40529-014-0066-2) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 70%

Fusaric acid induced cell death and changes in oxidative metabolism of Solanum lycopersicum L

Singh

Upadhyay

2014

Bot Stud

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“…The alkaline ceramidase mutant of Drosophila, known as Dacer, was shown to accumulate C14 and C16-sphingosines, and showed lower ROS production and greater resistance to oxidative stress than the wild type30. In tomato, a transgenic line with disrupted sphingolipid metabolism was shown to be sensitive to H 2 O 2 -associated programmed cell death (PCD)3132. Our data showed that knocked-down SlSLD expression resulted in reduced SOD and POD activities in tomato leaves under chilling stress (Fig.…”

Section: Discussionmentioning

confidence: 99%

The sphingolipid biosynthetic enzyme Sphingolipid delta8 desaturase is important for chilling resistance of tomato

Zhou

Zeng

et al. 2016

Sci Rep

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The physiological functions of sphingolipids in animals have been intensively studied, while less attention has been paid to their roles in plants. Here, we reveal the involvement of sphingolipid delta8 desaturase (SlSLD) in the chilling resistance of tomato (Solanum lycopersicum cv. Micro-Tom). We used the virus-induced gene silencing (VIGS) approach to knock-down SlSLD expression in tomato leaves, and then evaluated chilling resistance. Changes in leaf cell structure under a chilling treatment were observed by transmission electron microscopy. In control plants, SlSLD was highly expressed in the fruit and leaves in response to a chilling treatment. The degree of chilling damage was greater in SlSLD-silenced plants than in control plants, indicating that SlSLD knock-down significantly reduced the chilling resistance of tomato. Compared with control plants, SlSLD-silenced plants showed higher relative electrolytic leakage and malondialdehyde content, and lower superoxide dismutase and peroxidase activities after a chilling treatment. Chilling severely damaged the chloroplasts in SlSLD-silenced plants, resulting in the disruption of chloroplast membranes, swelling of thylakoids, and reduced granal stacking. Together, these results show that SlSLD is crucial for chilling resistance in tomato.

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“…A. thaliana has been described as a model organism for a number of mycotoxins, including fumonisin B1 (FB1) [22,23], AAL toxin [24] and the trichothecene family of toxins [25,26]. In our previous study, hypersensitive response-type lesions were observed in excised leaves infiltrated with OTA, such as the occurrence of oxidative bursts, phenolic compounds (autofluorescence) and deposition of callose [20].…”

Section: Introductionmentioning

confidence: 99%

iTRAQ Mitoproteome Analysis Reveals Mechanisms of Programmed Cell Death in Arabidopsis thaliana Induced by Ochratoxin A

Wang

Peng

Yang

et al. 2017

Toxins

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Ochratoxin A (OTA) is one of the most common and dangerous mycotoxins in the world. Previous work indicated that OTA could elicit spontaneous HR-like lesions formation Arabidopsis thaliana, reactive oxygen species (ROS) play an important role in OTA toxicity, and their major endogenous source is mitochondria. However, there has been no evidence as to whether OTA induces directly PCD in plants until now. In this study, the presence of OTA in Arabidopsis thaliana leaves triggered accelerated respiration, increased production of mitochondrial ROS, the opening of ROS-dependent mitochondrial permeability transition pores and a decrease in mitochondrial membrane potential as well as the release of cytochrome c into the cytosol. There were 42 and 43 significantly differentially expressed proteins identified in response to exposure to OTA for 8 and 24 h, respectively, according to iTRAQ analysis. These proteins were mainly involved in perturbation of the mitochondrial electron transport chain, interfering with ATP synthesis and inducing PCD. Digital gene expression data at transcriptional level was consistent with the cell death induced by OTA being PCD. These results indicated that mitochondrial dysfunction was a prerequisite for OTA-induced PCD and the initiation and execution of PCD via a mitochondrial-mediated pathway.

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An extensive microarray analysis of AAL-toxin-induced cell death in Arabidopsis thaliana brings new insights into the complexity of programmed cell death in plants

Cited by 129 publications

References 51 publications

Fusaric acid induced cell death and changes in oxidative metabolism of Solanum lycopersicum L

Fusaric acid induced cell death and changes in oxidative metabolism of Solanum lycopersicum L

The sphingolipid biosynthetic enzyme Sphingolipid delta8 desaturase is important for chilling resistance of tomato

iTRAQ Mitoproteome Analysis Reveals Mechanisms of Programmed Cell Death in Arabidopsis thaliana Induced by Ochratoxin A

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