Ratiometric imaging of lysosomal hypochlorous acid enabled by FRET-based polymer dots

Abstract

FRET-based fluorescent polymer dots (FPD) with good membrane permeability have been developed for ratiometric imaging of lysosomal HClO in living cells.

Protein‐Sized Dye‐Loaded Polymer Nanoparticles for Free Particle Diffusion in Cytosol

“…The limited brightness of organic fluorophores and fluorescent proteins, can be overcome by using fluorescent NPs . The most popular examples include quantum dots (QDs), dye‐doped silica NPs, NPs based on the assembly of dyes, conjugated polymer NPs, aggregation induced emission NPs, dye‐loaded polymer NPs, etc. The latter appeared recently as systems with exceptional brightness, which already found applications in super‐resolution imaging, amplified single‐molecule detection, and multicolor cellular imaging …”

A Three‐Color Fluorescent Supramolecular Nanoassembly of Phototherapeutics Activable by Two‐Photon Excitation with Near‐Infrared Light

“…In contrast to aw ide range of polymeric nanoparticles and self-assembling systems designed to observe FRET between co-entrapped multichromophoric guests, [15] in our case, these processes need to be strictly avoided. Only under such conditions, the three distinct chromo-fluorogenicm oieties can be operated in parallelu nder light inputs.T he fluorogenic moieties used in this work have been appropriately chosen to fulfil the above conditions, due to the negligible J-overlapb etween the emission band of potential energy donors and the absorption band of the potential energy acceptors (vide infra), which is the indispensable requisite for the FRET mechanism to occur.…”

A rational design of a cancer-specific and lysosome-targeted fluorescence nanoprobe for glutathione imaging in living cells

“…Alternatively, polymeric nanoparticles formed by amphiphilic diblock copolymers are promising candidates for constructing a dual-targeting GSH fluorescence probe due to the flexible structural design, suitable water solubility, favorable biocompatibility and so on. [39][40][41][42][43][44][45][46] Up to now, several polymeric nanoparticle-based dual-targeting nanoprobes have been developed for cancer imaging and therapy. [47][48][49][50][51][52][53][54] However, to the best of our knowledge, dualtargeting fluorescence polymeric nanoprobes for efficiently detecting lysosomal GSH within specific cancer cells have not yet been reported.…”