A rational design of a cancer-specific and lysosome-targeted fluorescence nanoprobe for glutathione imaging in living cells

Abstract

Developing versatile probe for targeting the lysosome of specific cancer cells and subsequently detecting glutathione (GSH) levels is critical in disclosing the roles of GSH in lysosomal oxidative stress of...

Fluorescent Probes for Live Cell Thiol Detection

“…In 2020, Hong Wang et al [211] built a dual targeting (cancer-specific and lysosometargeted) fluorescence nanoprobe (DTFN, Probe 83) for GSH imaging in live cells. The nanoprobe was constructed by combining folic acid-modified photostable aggregationinduced emission dots with manganese dioxide (MnO 2 ) nanosheets (GSH responsive site) through electrostatic interactions.…”

Near-Infrared Fluorescent Probe with a Large Stokes Shift for Detection of Hydrogen Sulfide in Food Spoilage, Living Cells, and Zebrafish

“…Among the reported methods for detecting H 2 S, the fluorescence-based assay has attracted public attention because of its real-time detection, simplicity of implementation, and nondestructive analysis, − and the fluorescent method has been applied in various areas such as food examination, biological imaging, and environmental monitoring. − For instance, Yan’s group reported a turn-on fluorescent probe which could target mitochondria, image H 2 S in living cells, and judge the H 2 S level in real water samples and beer; Xiao et al developed a color and fluorescence turn-on probe for monitoring the spoilage of eggs and fish; and Wang et al constructed a ratiometric and mitochondria-targeted probe, which not only could image endogenous and exogenous H 2 S in HeLa cells but also could measure the freshness of raw meat on-site . Although several fluorescent probes have been reported for detecting H 2 S, however, the majority of them is unable to visualize H 2 S in vivo primarily on account of their short emission wavelength (<650 nm), and only a few of the reported probes could detect H 2 S gas.…”

A nitrobenzoxadiazole-based near-infrared fluorescent probe for the specific imaging of H2S in inflammatory and tumor mice

“…Compared with these methods, fluorescent probes are extremely attractive because of their simple operation, high sensitivity, non-destructive analysis, and good compatibility with biological samples. 21–28 So far, various fluorescent probes for detecting H 2 S have been developed based on different fluorophores, such as rhodamine, 29 coumarin, 30 1,8-naphthalimide 31 and BODIPY. 32 However, the application of these probes in vivo is unsatisfactory because they have short emission wavelengths (<600 nm), resulting in high background signals and low tissue penetration.…”