Near-Infrared Fluorescent Probe with a Large Stokes Shift for Detection of Hydrogen Sulfide in Food Spoilage, Living Cells, and Zebrafish

Abstract

Hydrogen sulfide (H2S) is a significant component of various physiological processes, and it can also cause a negative effect on foodstuffs. In this work, we designed and synthesized an NIR fluorescent turn-on responding probe (DDM-H 2 S) with a large Stokes shift (190 nm) for the detection of H2S. DDM-H 2 S exhibited high selectivity and sensitivity, obvious color changes, and a fast response time for tracing H2S. When DDM-H 2 S reacted with H2S, the PET process was eliminated, and the recovered ICT process and NIR fluorescence were observed. Moreover, DDM-H 2 S could image endogenous and exogenous H2S in living HeLa cells and zebrafish. What is more, the probe DDM-H 2 S could be deposited easily to test paper strips, which were able to detect the H2S gas produced during food spoilage (such as eggs, raw meat, and fishes) by the color of test paper strips changing from pink to purple. Therefore, this work provides a promising approach for monitoring H2S in complicated biological systems and practical food samples.

Sustainable natural chlorogenic acid as a functional molecular sensor toward viscosity detection in liquids

“…Viscosity, an important micro-environment physical parameter, is a significant factor affecting the growth of bacteria, mold, and yeast since various nutrients (such as the cations, anions, glucose, vitamin, etc.) are included in the liquid system [1][2][3][4][5]. Abnormal changes in viscosity are associated with the spoilage extent of liquids, and a substantial portion of liquid safety is expected to be prevented through effective monitoring for timely intervention [6,7].…”

Novel 2-Benzo[d]thiazolyl-4-quinolinylphenol Skeleton-Based Turn-on Fluorescent Probe for H2S Detection and its Multiple Applications in Water Environment, Foodstuffs, and Living Organisms

“…For instance, the methylene blue method has a low detection sensitivity at the micromolar level. The fluorescent probe detection tool has obtained huge development thanks to its manifold advantages including high sensitivity, specific selectivity, low expenses, in situ and fast detection, robust compatibility, and scalability. − These advantages make the fluorescent probe method a hotspot field, and copious fluorescent chemosensors have also been developed for sensitively detecting specific analytes in vitro and vivo , food samples, and water environment. − These fluorescent probes often were designed based on the traditional fluorophores, such as coumarin, NBD, 1,8-naphthalimide, cyanine, pyrene, xanthene, etc. − To some degree, there were some developed fluorescent probes still existing a few drawbacks, such as using large amounts of organic solvents to realize the required emission signal, poor anti-interference ability and selectivity from other sulfur-containing analytes, requiring complicated operating procedures, and low synthesis productivity. − Therefore, to address the limitations enumerated above, it is vital and promising to develop novel small molecular fluorescent probes for specifically and on-the-spot monitoring of H 2 S in aqueous media …”

Triphenylamine indanedione as an AIE-based molecular sensor with one-step facile synthesis toward viscosity detection of liquids

“…[1][2][3] Commercial liquids are commonly composed of various nutritious additives, such as cations, anions, amino acids, small-molecular-weight glucose, and vitamin, and these basic components play key roles in maintaining homeostasis in liquids. 4,5 Moreover, the growth of bacteria, mold, and yeast depend on the aforementioned nutrients, which can cause obvious changes in the microenvironment during the deterioration process. 6 As an extremely important marker of the microenvironment, the viscosity of liquids plays an indispens-able role in the food deterioration process.…”