Rat subcutaneous tissue response to modified Portland Cement, a new mineral trioxide aggregate

Abstract

The purpose of this study was compare the biocompatibility of modified Portland cement (CPM) and mineral trioxide aggregate (MTA) in a subcutaneous rat model. Twenty-four male Wistar rats were used. Three silicon tubes were placed on the dorsal subcutaneous tissue of each animal: one tube contained MTA, one tube contained CPM and the other was an empty tube. The rats were sacrificed in 3 groups of 8 animals at 7, 14 and 30 postoperative days, respectively. Tissue samples were fixed in 10% buffered formalin, embedded in paraffin, and serial sections were cut and stained with hematoxylin and eosin, Masson Trichrome and Luna's stain. At day 7, the empty tubes displayed a mild inflammatory infiltrate. In the CPM group, an inflammatory infiltrate was observed with some eosinophils and immature connective tissue. The MTA group showed a similar infiltrate without eosinophils and presence of abundant necrotic tissue and numerous multinucleate foreign body giant cells. At day 14, the chronic infiltrate with eosinophils persisted when in contact with CPM. In the MTA group, necrosis and distant giant cells could still be seen. At day 30, all 3 groups showed mature fibrous collagenous tissue. These findings indicate a different response to the materials evaluated in this study. Although, MTA and CPM induced a chronic inflammatory infiltrate, necrosis and multinucleated foreign body giant cells predominated in the MTA group, while in the CPM group numerous eosinophils were seen at all the observational periods.

Evaluation of the biocompatibility of experimentally manufactured Portland cement: an animal study

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Evaluation of biocompatibility of a new root canal irrigant Q MixTM2 in 1- An in vivo study

“…However, the introduction of different materials in the subcutaneous cellular tissue or bone from small laboratory animals is widely regarded as a valid screening test for their biological properties. [17]…”

Biocompatibility of mineral trioxide aggregate and three new endodontic cements: An animal study

“…Previously, the biocompatibility of MTA and other calcium silicate cements has been proved. [5151626] It seems that the new material made from mixing two biocompatible cements (CAAC and calcium silicate) is still biocompatible.…”

“…Sections were evaluated under a light microscope (OLYMPUS CH30 RF200, Olympus Optical Co., Ltd. Japan) equipped with a digital camera (Sony ExwaveHAD, Tokyo, Japan) using 10× and 40× objective lenses by two independent examiners in a blind manner. The occurrence of inflammatory response were scored according to previously established scores[16] 0 (no reaction) for absence of inflammatory cells; 1+ (mild reaction) for presence of mild chronic inflammatory infiltrate, or few eosinophilic or giant cells; 2+ (moderate reaction) for presence of moderate chronic inflammatory infiltrate, or some eosinophilic or giant cells, or 3 + (severe reaction) for presence of an intense chronic inflammatory infiltrate, large number of eosinophilic or giant cells.…”