Rat strain differences in the activity of hepatic microsomal enzymes

Jori, A.; Pescador, Rodolfo; Pugliatti, C.

doi:10.1016/0006-2952(71)90179-1

Cited by 15 publications

(8 citation statements)

References 12 publications

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“…This is perhaps indicative of an increased drug sensitivity in female rats. Strain differences in drug-metabolizing enzyme activity have been reported with rabbits (Cram, Juchau & Fouts, 1965) and rats (Mitoma, Neubauer, Badger & Sorich, 1967;Jori et al, 1971). Stress has been reported to stimulate metabolism of, and reduce the response to, hexobarbitone, pentobarbitone and meprobamate.…”

Section: Discussionmentioning

confidence: 99%

A Study of the Factors Affecting the Sleeping Time Following Intracerebroventricular Administration of Pentobarbitone Sodium: Effect of Prior Administration of Centrally Active Drugs

Stevenson

Bull

1974

British J Pharmacology

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1 Injection of pentobarbitone sodium into a lateral cerebral ventricle of rats produced a loss of righting reflex. The duration of anaesthesia was dose-dependent. 2 The optimum dose of pentobarbitone to allow study of the factors affecting the sleeping time was considered to be 650 ,ug injected in 25 M water. 3 In a study of the effect of age and sex on the sleeping time, the youngest rats used (88 g body weight) were found to be the most sensitive to barbiturate. Female rats were more sensitive than male animals. 4 The duration of anaesthesia was not affected by induction or inhibition of hepatic drug-metabolizing enzyme activity. 5 Prior administration (acute) of central nervous system depressant drugs shortened the latent period and prolonged the duration of sleep. Prior administration of stimulant drugs antagonized the effect of pentobarbitone. 6 Animals withdrawn following chronic administration of a number of drugs, barbitone, barbitone/bemegride mixture, Mandrax (methaqualone: diphenhydramine; 10: 1), chlordiazepoxide, nitrazepam, chlorpromazine or ethanol, exhibited a significant tolerance to intracerebroventricularly administered pentobarbitone. 7 Withdrawal of amphetamine, morphine, methyprylon or diazepam did not result in tolerance to intracerebroventricularly administered pentobarbitone. 8 Chronic administration of all drugs except amphetamine and morphine induced a tolerance to intraperitoneally administered hexobarbitone (100 mg/kg). 9 The usefulness of sleeping time determination following intracerebroventricular administration of pentobarbitone as an assessment of central nervous system excitability is discussed. It is concluded that this method gives a valid indication of the sensitivity of the central nervous system to barbiturate and of the level of excitability in general. The method is particularly applicable in situations where the activity of hepatic drug-metabolizing enzyme activity may be altered.

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Section: Discussionmentioning

confidence: 99%

A Study of the Factors Affecting the Sleeping Time Following Intracerebroventricular Administration of Pentobarbitone Sodium: Effect of Prior Administration of Centrally Active Drugs

Stevenson

Bull

1974

British J Pharmacology

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“…It was previously reported from this laboratory that Long-Evans female rats metabolize several substrates to a different extent than do Sprague-Dawley rats [8,9,11]. It was also observed that the rate of drug metabolism significantly differs between stocks of Long-Evans rats obtained from different sources [11].…”

Section: Introductionmentioning

confidence: 78%

“…Enzymatic activity was measured on the 9,000 g supernatant fraction of liver homogenates, determining the amounts of metabolites, pN 02-phenol, pNH2-phenol and 4NH2-antipyrine formed from the added substrates pNO"-anisol, aniline and aminopyrine, respectively, as previously reported [11]. Metabolic activity in vivo was measured by evaluating the pharmacological activity (sleeping time) and the brain concentration of a given dose of pentobarbital (30 mg/kg i.p.)…”

Section: Methodsmentioning

confidence: 99%

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Differences in Microsomal Enzyme Induction between Sprague-Dawley and Long-Evans Rats

Jori¹,

Pugliatti²

1972

Pharmacology

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This study describes the effect of several inducing-agents on drug metabolism in Sprague-Dawley and Long-Evans rats. DDT, eucalyptol, and phenobarbital are less active in Long-Evans than in Sprague-Dawley rats. 3-Methylchol-anthrene, on the other hand, induces drug metabolism at similar extent in both strains. Experiments with phenobarbital show that the reduced reactivity of Long-Evans rats is dependent neither on the plasma and liver concentration of the inducing agent nor on time. However, a different reactivity to phenobarbital induction has been demonstrated in Long-Evans of 2 different sources.

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“…Von diesen Verfahren werden vor allem die zwei letztgenannten Methoden für die Eiweißbestimmung in Leberhomogenaten angewendet (3)(4)(5)(6)(7)(8). Vergleicht man jedoch die.…”

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Über den Einfluß von Fett auf die Proteinbestimmung mit Biuret- und Folin-Ciocalteus Phenol-Reagenz in Leberhomogenaten

Metz¹,

Reinert²

2009

Clinical Chemistry and Laboratory Medicine

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Die vorliegende Studie behandelt die Problematik der photometrischen Proteinbestimmung in Leberhomogenaten in Anwesenheit steigender Fettmengen und zeigt die dabei auftretenden Fehlermöglichkeiten auf. Es wird vorgeschlagen, diese Fettrübungseinflüsse entweder durch Detergenzzusatz (Desoxycholat) oder durch Verwendung von Spektralphotometern mit speziellen Meßanordnungen für Trübungsmessungen zu unterdrücken. The influence of fat on the determination of protein by the biuret and the Folin-Ciocalteau methods in liver homogenatesThe present study deals with the spectrophotometric determination of protein in liver homogenates and the errors of determination liable to occur due to clouding of the test sample by the presence of lipids. Two ways of suppressing this source of error are described: the addition of a detergent (desoxycholate) to the test sample to clear the clouding, and the use of appropriate optical equipment to correct the scattering effect Of the clouding on the light beam being measured.

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Rat strain differences in the activity of hepatic microsomal enzymes

Cited by 15 publications

References 12 publications

A Study of the Factors Affecting the Sleeping Time Following Intracerebroventricular Administration of Pentobarbitone Sodium: Effect of Prior Administration of Centrally Active Drugs

A Study of the Factors Affecting the Sleeping Time Following Intracerebroventricular Administration of Pentobarbitone Sodium: Effect of Prior Administration of Centrally Active Drugs

Differences in Microsomal Enzyme Induction between Sprague-Dawley and Long-Evans Rats

Über den Einfluß von Fett auf die Proteinbestimmung mit Biuret- und Folin-Ciocalteus Phenol-Reagenz in Leberhomogenaten

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