Rare‐Cell Enrichment by a Rapid, Label‐Free, Ultrasonic Isopycnic Technique for Medical Diagnostics

Bourquin, Yannyk; Syed, Abeer; Reboud, Julien; Ranford-Cartwright, Lisa; Barrett, Michael P.; Cooper, Jonathan M.

doi:10.1002/ange.201310401

Cited by 15 publications

(26 citation statements)

References 20 publications

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“…Depending on the cell size and density, this type of flow allowed the concentration of specific cells in the center of a reactor while forcing others to the periphery. 17,18 ■ CONCLUSIONS…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Acoustofluidics and Whole-Blood Manipulation in Surface Acoustic Wave Counterflow Devices

et al. 2014

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On-chip functional blocks for sample preprocessing are necessary elements for the implementation of fully portable micrototal analysis systems (μTAS). We demonstrate and characterize the microparticle and whole-blood manipulation capabilities of surface acoustic wave (SAW) driven counterflow micropumps. The motion of suspended cells in this system is governed by the two dominant acoustic forces associated with the scattered SAW (of wavelength λf): acoustic-radiation force and acoustic-streaming Stokesian drag force. We show that by reducing the microchannel height (h) beyond a threshold value the balance of these forces is shifted toward the acoustic-radiation force and that this yields control of two different regimes of microparticle dynamics. In the regime dominated by the acoustic radiation force (h ≲ λf), microparticles are collected in the seminodes of the partial standing sound-wave arising from reflections off microchannel walls. This enables the complete separation of plasma and corpuscular components of whole blood in periodical predetermined positions without any prior sample dilution. Conversely, in the regime dominated by acoustic streaming (h ≫ λf), the microbeads follow vortical streamlines in a pattern characterized by three different phases during microchannel filling. This makes it possible to generate a cell-concentration gradient within whole-blood samples, a behavior not previously reported in any acoustic-streaming device. By careful device design, a new class of SAW pumping devices is presented that allows the manipulation and pretreatment of whole-blood samples for portable and integrable biological chips and is compatible with hand-held battery-operated devices.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Acoustofluidics and Whole-Blood Manipulation in Surface Acoustic Wave Counterflow Devices

et al. 2014

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“…A schematic model system illustrates the collision of the cells and microparticles with different concentrations ( Figure a) . Here, residue stress analysis of the dense cells and microparticles is given in Figure b,d.…”

Section: Resultsmentioning

confidence: 99%

“…A schematic model system illustrates the collision of the cells and microparticles with different concentrations (Figure 2a). [39][40][41] Here, residue stress analysis of the dense cells and microparticles is given in Figure 2b,d. Then, the dense cells and microparticles were accelerated by the residual force in the form of circular movement within the acoustic streaming field with the aid of tapes (Figure 2c,e).…”

Section: Schematic Of the Cell-microparticle Collisionsmentioning

confidence: 99%

Piezoelectric Microchip for Cell Lysis through Cell–Microparticle Collision within a Microdroplet Driven by Surface Acoustic Wave Oscillation

Wang

et al. 2019

Small

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Cell lysis is an important and crucial step for the detection of intracellular secrets. Usually, cell lysis is based on strong ultrasonic waves or toxic chemical regents, which require a large amount of cell suspension. To obtain high efficiency cell lysis for a small amount of sample, a mechanical cell lysis method based on a surface acoustic wave (SAW) microchip is proposed. The microchip simply consists of a piece of LiNbO3 crystal substrate, interdigitated transducers (IDTs) with 80 pairs of parallel electrodes and 3M Magic Tapes. The modulated input electrical signal is coupled into the substrate through IDTs, which produces an acoustic stream in the droplet on the surface of a substrate. When a biofluid droplet containing cells and microparticles is dropped on the surface of the microchip, the cells and microparticles are accelerated and collide with each other. The fluorescence staining results illustrate that the cell membrane is efficiently destroyed and that proteins as well as nucleic acids inside the cell are released. The experimental results show that this method has a high efficiency and low sample consumption. The potential application is the pretreatment of a small amount of tested sample in a hospital or biolab.

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“…TSAW-induced acoustic streaming has also been used for size-independent separation. Bourquin et al applied TSAW-induced acoustic streaming to separate and enrich circulating cells infected by parasite from non-parasitized red blood cells (RBCs), enabling detection at low levels of infection on chips [ 61 ]. Since the density of infected RBCs is lower than the uninfected RBCs, the denser cells (uninfected cells) were accumulated at the center while the lighter cells (infected cells) moved to the periphery via acoustic streaming-induced drag force ( Figure 3 b).…”

Section: Saw-based Separationmentioning

confidence: 99%

Acoustic Microfluidic Separation Techniques and Bioapplications: A Review

Gao

Lin

et al. 2020

Micromachines

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Microfluidic separation technology has garnered significant attention over the past decade where particles are being separated at a micro/nanoscale in a rapid, low-cost, and simple manner. Amongst a myriad of separation technologies that have emerged thus far, acoustic microfluidic separation techniques are extremely apt to applications involving biological samples attributed to various advantages, including high controllability, biocompatibility, and non-invasive, label-free features. With that being said, downsides such as low throughput and dependence on external equipment still impede successful commercialization from laboratory-based prototypes. Here, we present a comprehensive review of recent advances in acoustic microfluidic separation techniques, along with exemplary applications. Specifically, an inclusive overview of fundamental theory and background is presented, then two sets of mechanisms underlying acoustic separation, bulk acoustic wave and surface acoustic wave, are introduced and discussed. Upon these summaries, we present a variety of applications based on acoustic separation. The primary focus is given to those associated with biological samples such as blood cells, cancer cells, proteins, bacteria, viruses, and DNA/RNA. Finally, we highlight the benefits and challenges behind burgeoning developments in the field and discuss the future perspectives and an outlook towards robust, integrated, and commercialized devices based on acoustic microfluidic separation.

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Rare‐Cell Enrichment by a Rapid, Label‐Free, Ultrasonic Isopycnic Technique for Medical Diagnostics

Cited by 15 publications

References 20 publications

Acoustofluidics and Whole-Blood Manipulation in Surface Acoustic Wave Counterflow Devices

Acoustofluidics and Whole-Blood Manipulation in Surface Acoustic Wave Counterflow Devices

Piezoelectric Microchip for Cell Lysis through Cell–Microparticle Collision within a Microdroplet Driven by Surface Acoustic Wave Oscillation

Acoustic Microfluidic Separation Techniques and Bioapplications: A Review

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