Rapid serum vancomycin assay by high-performance liquid chromatography using a semipermeable surface packing material column

Furuta, Itaru; Kitahashi, Toshihiro; Kuroda, Takayoshi; Nishio, Hiroshi; Oka, Chizu; Morishima, Yasuo

doi:10.1016/s0009-8981(00)00307-7

Cited by 30 publications

(28 citation statements)

References 10 publications

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“…Solid-phase extraction, as used by Greene et al (1987), was replaced by a less timeconsuming method of sample preparation. The LLOQ found in plasma conforms to other LLOQs reported for vancomycin in bioanalytical assays using HPLC with UV detection (Farin et al, 1998;Furuta et al, 2000) or was even lower (Li et al, 1995;Greene et al, 1987;Backes et al, 1998;Demotes-Mainard et al, 1994;Hu et al, 1990;Luksa and Marusic, 1995). More sensitive methods have been reported using tandem mass spectrometry for detection (Cass et al, 2001;Shibata et al, 2003).…”

Section: Resultssupporting

confidence: 81%

Successful management of discovered pH dependence in vancomycin recovery studies: novel HPLC method for microdialysis and plasma samples

Plock¹,

Buerger²,

Kloft³

2005

Biomed. Chromatogr.

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Vancomycin is a glycopeptide antibiotic approved for the treatment of serious infections or patients allergic to beta-lactams. A rapid HPLC assay using UV detection for the determination in microdialysate and human plasma was developed. After sample preparation, using methanol and trichloroacetic acid for plasma and water for microdialysate, 20 microL were injected and separated on a RP(18) column. Overall, the assay exhibited good precision and accuracy. The diffusion properties of vancomycin investigated in in vitro microdialysis experiments revealed an unfavourable concentration dependence avertable by keeping a constant pH using phosphate buffer as perfusate. The mean relative recoveries were 27.8% [coefficient of variation (CV) 11.1%] and 33.2% (CV 8.3%) for retrodialysis and recovery experiments, respectively. Following characterization of vancomycin in in vitro microdialysis, the developed setting is suitable for application in (pre-)clinical studies.

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Section: Resultssupporting

confidence: 81%

Successful management of discovered pH dependence in vancomycin recovery studies: novel HPLC method for microdialysis and plasma samples

Plock¹,

Buerger²,

Kloft³

2005

Biomed. Chromatogr.

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“…were tested and compared [8][9][10][11][12][13][14][15][16][17][18][19]. The results suggested that acetonitrile-acid aqueous solution was superior to the others.…”

Section: Optimization Of Chromatographic Conditionmentioning

confidence: 99%

A Simple and Modified Method Development of Vancomycin Using High Performance Liquid Chromatography

Chauhan¹,

Bhatt²

2015

J Chromatogr Sep Tech

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“…For endocarditis caused by enterococci (e.g., Enterococcus faecalis), vancomycin hydrochloride has been reported to be effective only in combination with an aminoglycoside. Vancomycin can be determined by several analytical methods, such as voltametric [26], spectrophotometric [27], radioimmunoassay and fluorescence polarization immunoassay [28], LC [29], and HPLC [30][31][32][33][34][35][36][37].…”

Section: Introductionmentioning

confidence: 99%

Indirect spectrophotometric determination of gentamicin and vancomycin antibiotics based on their oxidation by potassium permanganate

et al. 2006

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Four simple, accurate, sensitive and economical procedures (A -D) for the estimation of gentamicin sulphate and vancomycin hydrochloride, both in pure form and in pharmaceutical formulations have been developed. The methods are based on the oxidation of the studied drugs by a known excess of potassium permanganate in sulphuric acid medium and subsequent determination of unreacted oxidant by reacting it with amaranth dye (method A), acid orange II (method B), indigocarmine (method C) and methylene blue (method D), in the same acid medium at a suitable λ max =521, 485, 610 and 664 nm, respectively. The reacted oxidant corresponds to the drug content. Regression analysis of Beer-Lambert plots showed good correlations in the concentration ranges 4-8, 3-8, 4-9 and 5-9 µg ml −1 with gentamicin and 4-8, 1.5-4, 1.5-4 and 3.5-5.5 µg ml −1 with vancomycin for methods A, B, C, and D, respectively. The molar absorptivity, sandell sensitivity, detection and quantification limits were calculated. The stoichiometric ratios for the cited drugs were studied. The optimum reaction conditions and other analytical parameters were evaluated. The influence of the substance commonly employed as excipients with these drugs were studied. The proposed methods were applied to the determination of these drugs in pharmaceutical formulations. The results have demonstrated that the methods are equally accurate and reproducible as the official methods.

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Rapid serum vancomycin assay by high-performance liquid chromatography using a semipermeable surface packing material column

Cited by 30 publications

References 10 publications

Successful management of discovered pH dependence in vancomycin recovery studies: novel HPLC method for microdialysis and plasma samples

Successful management of discovered pH dependence in vancomycin recovery studies: novel HPLC method for microdialysis and plasma samples

A Simple and Modified Method Development of Vancomycin Using High Performance Liquid Chromatography

Indirect spectrophotometric determination of gentamicin and vancomycin antibiotics based on their oxidation by potassium permanganate

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