Indirect spectrophotometric determination of gentamicin and vancomycin antibiotics based on their oxidation by potassium permanganate

Abstract

Four simple, accurate, sensitive and economical procedures (A -D) for the estimation of gentamicin sulphate and vancomycin hydrochloride, both in pure form and in pharmaceutical formulations have been developed. The methods are based on the oxidation of the studied drugs by a known excess of potassium permanganate in sulphuric acid medium and subsequent determination of unreacted oxidant by reacting it with amaranth dye (method A), acid orange II (method B), indigocarmine (method C) and methylene blue (method D), in the same acid medium at a suitable λ max =521, 485, 610 and 664 nm, respectively. The reacted oxidant corresponds to the drug content. Regression analysis of Beer-Lambert plots showed good correlations in the concentration ranges 4-8, 3-8, 4-9 and 5-9 µg ml −1 with gentamicin and 4-8, 1.5-4, 1.5-4 and 3.5-5.5 µg ml −1 with vancomycin for methods A, B, C, and D, respectively. The molar absorptivity, sandell sensitivity, detection and quantification limits were calculated. The stoichiometric ratios for the cited drugs were studied. The optimum reaction conditions and other analytical parameters were evaluated. The influence of the substance commonly employed as excipients with these drugs were studied. The proposed methods were applied to the determination of these drugs in pharmaceutical formulations. The results have demonstrated that the methods are equally accurate and reproducible as the official methods.

Analytical methods for the determination of certain antibiotics used in critically ill patients

“…El-Didamony et al, performed four procedures for the determination of Gentamicin Sulphate and Vancomycin Hydrochloride, both in pure form and in pharmaceutical dosage forms. The methods are based on the oxidation of the studied drugs by a known excess of potassium permanganate in sulphuric acid medium and subsequent determination of unreacted oxidant by reacting it with amaranth dye (method A), acid orange II (method B), indigocarmine (method C) and methylene blue (method D), in the same acid medium at a suitable λ max = 521, 485, 610 and 664 nm, respectively [106] .…”

Penetapan kadar gentamisin dalam sediaan krim dengan kromatografi lapis tipis - densitometri

“…Metode ini juga dilaporkan untuk penetapan kadar gentamisin (kombinasi dengan meropenem) dalam serum dan plasma (Zuzanna et al, 2006). Analisis kuantitatif gentamisin terutama untuk tujuan pengendalian mutu sediaan telah dikembangkan dengan berbagai metode, misalnya kromatografi kinerja tinggi (KCKT) (Isoherranen, 2000;Kuehl, 2010), dan Omar et al (2013) juga melaporkan metode spektrofotometri untuk penetapan kadar gentamisin dalam calam campurannya dengan vankomisin, namun membutuhkan tahapan yang tidak sederhana (Clarot et al, 2004;El-Didamony et al, 2006;Gubernator et al, 2006).…”

Flow‐injection chemiluminescence determination of gentamicin: optimization by central composite design

“…However, due to a lack of any significant chromophore or fluorophore in gentamicin, determination of this drug is difficult (3). A literature survey reveals several analytical methods for the quantification of gentamicin including: HPLC (4-6), capillary electrophoresis (7), mass spectrometry (8)(9)(10)(11)(12), immunoassay (13,14), fluorometry (15,16), spectrophotometry (17,18), potentiometry (19), electrogenerated chemiluminescence (20) and chemiluminescence (21,22) methods (Table 1).…”