Rapid separation of fatty acids using a poly(vinyl alcohol) coated capillary in nonaqueous capillary electrophoresis with contactless conductivity detection

Buglione, Lucia; See, Hong Heng; Hauser, Peter C.

doi:10.1002/elps.201300028

Cited by 26 publications

(22 citation statements)

References 52 publications

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“…The highest S/N ratio was obtained for NaDCHA, mainly due to very low baseline noise. This finding is in good accordance with previous reports [29,30]. Complete baseline separation of all target analytes including endoxifen racemate was achieved within 6 min, which is to our knowledge the fastest separation ever reported using the NACE method.…”

Section: Buffer Saltsupporting

confidence: 93%

Determination of tamoxifen and its metabolites in human plasma by nonaqueous capillary electrophoresis with contactless conductivity detection

2015

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A new approach for the quantification of tamoxifen and its metabolites 4-hydroxytamoxifen, N-desmethyltamoxifen, and 4-hydroxy-N-desmethyltamoxifen (endoxifen) in human plasma samples using NACE coupled with contactless conductivity detection (C D) is presented. The buffer system employed consisted of 7.5 mM deoxycholic acid sodium salt, 15 mM acetic acid, and 1 mM 18-crown-6 in 100% methanol. The complete separation of all targeted compounds (including endoxifen racemate) could be achieved within 6 min under optimized conditions. The proposed method was validated and showed good linearity in the range from 100 to 5000 ng/mL with correlation coefficients between 0.9922 and 0.9973, LODs in the range of 25-40 ng/mL, and acceptable reproducibility of the peak area (intraday RSD 2.2-3.1%, n = 4; interday (3 days) RSD 6.0-8.8%, n = 4). The developed method was successfully demonstrated for the quantification of tamoxifen and its metabolites in human plasma samples collected from breast cancer patients undertaking tamoxifen treatment.

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Section: Buffer Saltsupporting

confidence: 93%

Determination of tamoxifen and its metabolites in human plasma by nonaqueous capillary electrophoresis with contactless conductivity detection

2015

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“…Another innovative analytical approach for the analysis of FAs was developed by Buglione et al. . A total of 12 FAs could be separated using a NACE method with LOD of 0.5 μM for all FAs determined.…”

Section: Lipidsmentioning

confidence: 99%

“…In this context, a CE method for FA determination in soybean oil, sunflower seed oil, and maize oil was developed [97]. Another innovative analytical approach for the analysis of FAs was developed by Buglione et al [98]. A total of 12 FAs could be separated using a NACE method with LOD of 0.5 M for all FAs determined.…”

Section: Lipidsmentioning

confidence: 99%

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

et al. 2015

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This review work presents and discusses the main applications of capillary electromigration methods in food analysis and Foodomics. Papers that were published during the period February 2013-February 2015 are included following the previous review by Garcia-Cañas et al. (Electrophoresis, 2014, 35, 147-169). Analysis by CE of a large variety of food-related molecules with different chemical properties, including amino acids, hazardous amines, peptides, proteins, phenols, polyphenols, lipids, carbohydrates, DNAs, vitamins, toxins, contaminants, pesticides, residues, food additives, as well as small organic and inorganic compounds. This work includes recent results on food quality and safety, nutritional value, storage, bioactivity, as well as applications of CE for monitoring food processing. The use, among other CE developments, of microchips, CE-MS, and chiral CE in food analysis and Foodomics is also discussed.

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“…The use of MEKC was also explored for the separation of saturated and unsaturated FFAs (within the C14 to C18 range) in the presence of polyoxyethylene-23-dodecylether (Brij 35) micelles and with the use of methanol as the [8] FFA C14:0, C16:0,C16:, C18:0;C18:1, C18:2,C18:. [30] FFA C20:0, C18:0, C18:1t, C18:1c, C18:2, C18:3, C16:0, C15:0, C14:0, C13:0, C12:0. Omega-3 and omega-6 FAs : C18:3 n-3, C18:3 n-6, C18:2, n-6, C19:2 n-6, C20:4 n-6, C20:2 n-6, C20:5 n-3, C22:6 n-3, C20:3 n-3, C20:3 n-6, C22:5 n-3, C22:5 n-6, C22:4 n-6, C22:3 n-3, C22:2 n-6, electrolyte [5].…”

Section: Uv Detectionmentioning

confidence: 99%

A digest of capillary electrophoretic methods applied to lipid analyzes

Poinsot

Meang

et al. 2018

Electrophoresis

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Lipids are naturally occurring organic compounds that can be classified into a number of types based on their solubility in nonpolar organic solvents, and are generally insoluble in water. The great structural variety of these various types of lipids has led them to be components of many different biological substances such as oils, waxes, cellular membranes, tissues and biological fluids. The use of capillary electrophoresis (CE) for the study of lipids during the past 30 years has been relatively rare when compared to its use for other classes of biomolecules, primarily due to their insolubility in water. However, a number of interesting studies have been conducted, and as part of this review, we will present the different approaches that were used, which mainly consist of micellar kinetic chromatography and non-aqueous CE. The main advantages of the use of these techniques compared to GC is the very simple sample preparation that is required and, compared to LC, the very robust and quick separations that can be obtained. In this review, we present the various methods that have been reported in the literature that have been used for the study of fatty acids, phospholipids, glycerides, eicosanoids and sterols, with the inclusion of various tables presenting descriptions of the CE methods used as well as the methods of detection, including UV absorbance, fluorescence, mass spectrometry, and conductivity. This review aims to demonstrate that CE can be easily used for the analysis of lipids.

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Rapid separation of fatty acids using a poly(vinyl alcohol) coated capillary in nonaqueous capillary electrophoresis with contactless conductivity detection

Cited by 26 publications

References 52 publications

Determination of tamoxifen and its metabolites in human plasma by nonaqueous capillary electrophoresis with contactless conductivity detection

Determination of tamoxifen and its metabolites in human plasma by nonaqueous capillary electrophoresis with contactless conductivity detection

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

A digest of capillary electrophoretic methods applied to lipid analyzes

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