Rapid quantitative analysis of adulterant Lonicera species in preparations of Lonicerae Japonicae Flos

Abstract

Lonicerae Japonicae Flos is often adulterated with Lonicerae Flos, which is derived from the other four Lonicera species, in both the crude drug and Lonicerae Japonicae Flos preparations. We proposed a methodology for the quantitative analysis of adulterant Lonicerae Flos in Lonicerae Japonicae Flos preparations. Taking macranthoidins A, B, dipsacoside B (saponins), sweroside (iridoids), and luteolin-7-O-d-glucoside (flavonoids) as markers, a method of ultra high performance liquid chromatography with triple quadrupole mass spectrometry was employed to determine their amounts in Lonicerae Flos, Lonicerae Japonicae Flos, and Lonicerae Japonicae Flos preparations. The proportion of adulterant Lonicerae Flos in Lonicerae Japonicae Flos preparations was estimated based on the saponin contents of Lonicerae Japonicae Flos and Lonicerae Flos. All analytes separated under isocratic elution in 12 min with acceptable linearity, precision, repeatability, and accuracy. Lonicerae Japonicae Flos was easily distinguished from Lonicerae Flos by the total amount of saponins (0.067 and > 45.8 mg/g for Lonicerae Japonicae Flos and Lonicerae Flos, respectively). Eighteen of twenty one Lonicerae Japonicae Flos preparation samples were adulterated with Lonicerae Flos in proportions of 11.3-100%. The developed ultra high performance liquid chromatography with triple quadrupole mass spectrometry method could be used for the identification of Lonicerae Japonicae Flos and the four species of Lonicerae Flos and for the analysis of Lonicerae Japonicae Flos preparations adulterated with Lonicerae Flos.

Lonicerae japonicae flos and Lonicerae flos: a systematic review of ethnopharmacology, phytochemistry and pharmacology

“…Centauroside (146) and (E)-aldosecologanin (185) exhibited much more potent NO inhibitory activities than the positive control minocycline in vitro (IC 50-= 20.07 ± 0.37 lM), with IC 50 values of 7.96 ± 0.47 and 12.60 ± 1.50 lM, respectively. Zhang et al (2015) tr trace, nd not detect, nr no record What's more, neither of them showed significant cytotoxicity at the concentration of 100 lM . In this literature, it also mentioned that secoiridoid glycosides had a more positive effect on a-glucosidase inhibition than other iridoid glycosides, while the presence of a methoxy group at C-7 or a double bond at C-6 or C-7 appeared to reduce the inhibition markedly.…”

“…A large number of studies indicate that saponins contented in JYH are fewer than those in SYH Chai et al 2005;Zhang et al 2015;Yang et al 2016). Saponins are the most compounds in SYH (Fig.…”

Derivative Technology of DNA Barcoding (Nucleotide Signature and SNP Double Peak Methods) Detects Adulterants and Substitution in Chinese Patent Medicines

“…L. fulvotomentosa ) , L. confusa, L. hypoglauca , and L. macranthoides . Lonicerae Flos is much cheaper than Lonicerae japonicae Flos 14 . According to the theory of traditional Chinese medicine characteristics, Eucommiae Folium and Lonicerae japonicae Flos have totally different pharmacology, so the two herbal materials should be clearly distinguished.…”

Quality evaluation of Lonicerae Japonicae Flos and Lonicerae Flos based on simultaneous determination of multiple bioactive constituents combined with multivariate statistical analysis

“…They are typical Chinese medicines of "heterologous effect". Numerous literature has reported that LF has similar pharmacological activities as LJF, 8,11 only with a marginal difference in the strength of the drug effect. But until now, there is still no recognised theory to explain this phenomenon.…”