Rapid quantification of microalgal lipids in aqueous medium by a simple colorimetric method

Mishra, Sanjiv K.; Suh, William I.; Farooq, Wasif; Moon, Myounghoon; Shrivastav, Anupama; Park, Min; Yang, Ji‐Won

doi:10.1016/j.biortech.2013.12.077

Cited by 330 publications

(158 citation statements)

References 22 publications

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“…Subsequently 50 ml of concentrated H 2 SO 4 was added to the mixture, and the resulting reagent was stored in the dark until use. To ensure high activity, fresh phospho-vanillin reagent was prepared shortly before every experiment [23].…”

Section: Sulfo-phospho-vanillin Assay For Lipid Estimationmentioning

confidence: 99%

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Accumulation of lipid in Dunaliella salina under Nutrient Starvation Condition

Mai¹,

Nguyen²,

Vo³

et al. 2017

AJFN

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The effect of nutrient starvation on lipid accumulation of Dunaliella salina A9 was studied. In nutrient starvation, cell colour changed from green to yellow (or orange) and cell growth reached stationary phase after 9 days of the culture. The study showed that under nutrient stress, decreased in cell growth is accompanied by carotenoid biosynthesis and lipid content of Dunaliella salina. The results of this study can be used to increase carotenoid and lipid production in microalgae for functional food and biofuel in the future.

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Section: Sulfo-phospho-vanillin Assay For Lipid Estimationmentioning

confidence: 99%

“…5 mL of freshly prepared phospho-vanillin reagent was then added, and the sample was incubated for 15 min at 37°C incubator shaker at 200 rpm. Absorbance reading at 530 nm was taken in order to quantify lipid content of the sample [23].…”

Section: Sulfo-phospho-vanillin Assay For Lipid Estimationmentioning

confidence: 99%

Accumulation of lipid in Dunaliella salina under Nutrient Starvation Condition

Mai¹,

Nguyen²,

Vo³

et al. 2017

AJFN

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Section: Color Reaction Of Four Dinoflagellate Species In Different Cmentioning

confidence: 99%

“…The advantages of this technique are that it is able to measure lipid content rapidly and simply and requires only a small amount of target sample. Thus, this method has been employed for rapid quantification of the intracellular lipid contents of some microalgae (Hao et al 2013, Mishra et al 2014, Byreddy et al 2016.In this study, we applied a modified SPV method for quantifying the lipid content of dinoflagellate species. Furthermore, using this method, we analyzed the lipid content of 9 dinoflagellate species including phototroph- ) was supplied to the predator cells every day for 5 days, but not on the last day, in order to eliminate all yeast from the O. marina culture medium.…”

mentioning

confidence: 99%

Fatty acid composition and docosahexaenoic acid (DHA) content of the heterotrophic dinoflagellate Oxyrrhis marina fed on dried yeast: compared with algal prey

Yoon¹,

Park²,

Jeong³

et al. 2017

ALGAE

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To develop an easy and rapid method of quantifying lipid contents of marine dinoflagellates, we quantified lipid contents of common dinoflagellate species using a colorimetric method based on the sulpho-phospho-vanillin reaction. In this method, the optical density measured using a spectrophotometer was significantly positively correlated with the known lipid content of a standard oil (Canola oil). When using this method, the lipid content of each of the dinoflagellates Alexandrium minutum, Prorocentrum micans, P. minimum, and Lingulodinium polyedrum was also significantly positively correlated with the optical density and equivalent intensity of color. Thus, when comparing the color intensity or the optical density of a sample of a microalgal species with known color intensities or optical density, the lipid content of the target species could be rapidly quantified. Furthermore, the results of the sensitivity tests showed that only 1-3 × 10 5 cells of P. minimum and A. minutum, 10 4 cells of P. micans, and 10 3 cells of L. polyedrum (approximately 1-5 mL of dense cultures) were needed to determine the lipid content per cell. When the lipid content per cell of 9 dinoflagellates, a diatom, and a chlorophyte was analyzed using this method, the lipid content per cell of these microalgae, with the exception of the diatom, were significantly positively correlated with cell size, however, volume specific lipid content per cell was negatively correlated with cell size. Thus, this sulpho-phospho-vanillin method is an easy and rapid method of quantifying the lipid content of autotrophic, mixotrophic, and heterotrophic dinoflagellate species.Key Words: cell size; intensity of color; lipid amount; lipid content per cell; sulfo-phospho-vanilin (SPV); volume specific lipid content INTRODUCTIONKnowing the lipid content of marine microalgae is important for understanding their metabolism and for the use of their biomass as biofuel. Among them, dinoflagellates contain large amounts of high-quality lipids and dinosterols (Piretti et al. 1997, Mansour et al. 1999.Dinoflagellates play diverse roles in the marine food web as primary producers, prey, predators, symbionts, and parasites (Muscatine 1990, Coats 1999, Jeong et al. 2010, Lee et al. 2014a, 2014b. In the last two decades, many phototrophic dinoflagellates that were previously thought to be exclusively autotrophic have been shown to be mixotrophic (Jacobson and Anderson 1996, Stoecker 1999, Jeong et al. 2005, Lee et al. 2014c. Therefore, these protists not only perform photosynthesis, but also feed on prey as energy sources. Furthermore, many dinoflagellates are important prey for mixotrophic Received October 3, 2016, Accepted December 7, 2016 *Corresponding Author E-mail: bada0@snu.ac.kr Tel: This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original wo...

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“…Subsequently 50 ml of concentrated phosphoric acid was added to the mixture, and the resulting reagent was stored in the dark until use. To ensure high activity, fresh phospho-vanillin reagent was prepared shortly before every experiment run [26].…”

Section: Sulfo-phospho-vanillin Assay For Lipid Accumulationmentioning

confidence: 99%

Effect of Osmotic Stress and Nutrient Starvation on the Growth, Carotenoid and Lipid Accumulation in Dunaliella salina A9

Vo¹,

Mai²,

Vu³

et al. 2017

PLANT

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Dunaliella salina A9 is unicellular green alga isolated from the saltern, Khanh Hoa province, Viet Nam.The effect of halostress and nutrient starvation was studied in this alga to estimate the growth, chlorophyll content and capacity of carotenoid and lipid accumulation. The results showed decrease in cell number and chlorophyll content as Dunaliella salina in response to a change from the optimal medium 1.5M NaCl to hypo-osmotic medium (0.5M NaCl) and hyper-osmotic medium (3.5M NaCl). We also observed decrease in cell count in nutrient starvation after 9 days of culture in MD4 medium. Salinity stress has more severe effect on the growth of Dunaliella salina A9 with greater decrease in cell number compared to nutrient starvation. The stress induced increasing carotenoid and lipid accumulation in cells. However the carotenoid and lipid accumulation in hypo-osmotic stress and the nutrient starvation were higher than in hyper-osmotic stress. The results suggested negative relationship between the growth rate, chlorophyll content and carotenoid, and lipid accumulation of Dunaliella salina under stress conditions.

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Rapid quantification of microalgal lipids in aqueous medium by a simple colorimetric method

Cited by 330 publications

References 22 publications

Accumulation of lipid in <i>Dunaliella salina </i>under Nutrient Starvation Condition

Accumulation of lipid in <i>Dunaliella salina </i>under Nutrient Starvation Condition

Fatty acid composition and docosahexaenoic acid (DHA) content of the heterotrophic dinoflagellate Oxyrrhis marina fed on dried yeast: compared with algal prey

Effect of Osmotic Stress and Nutrient Starvation on the Growth, Carotenoid and Lipid Accumulation in <i>Dunaliella salina </i>A9

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