The effect of nutrient starvation on lipid accumulation of Dunaliella salina A9 was studied. In nutrient starvation, cell colour changed from green to yellow (or orange) and cell growth reached stationary phase after 9 days of the culture. The study showed that under nutrient stress, decreased in cell growth is accompanied by carotenoid biosynthesis and lipid content of Dunaliella salina. The results of this study can be used to increase carotenoid and lipid production in microalgae for functional food and biofuel in the future.
Dunaliella salina A9 is unicellular green alga isolated from the saltern, Khanh Hoa province, Viet Nam.The effect of halostress and nutrient starvation was studied in this alga to estimate the growth, chlorophyll content and capacity of carotenoid and lipid accumulation. The results showed decrease in cell number and chlorophyll content as Dunaliella salina in response to a change from the optimal medium 1.5M NaCl to hypo-osmotic medium (0.5M NaCl) and hyper-osmotic medium (3.5M NaCl). We also observed decrease in cell count in nutrient starvation after 9 days of culture in MD4 medium. Salinity stress has more severe effect on the growth of Dunaliella salina A9 with greater decrease in cell number compared to nutrient starvation. The stress induced increasing carotenoid and lipid accumulation in cells. However the carotenoid and lipid accumulation in hypo-osmotic stress and the nutrient starvation were higher than in hyper-osmotic stress. The results suggested negative relationship between the growth rate, chlorophyll content and carotenoid, and lipid accumulation of Dunaliella salina under stress conditions.
Solanum torvum Swartz, belonging to the family Solanaceae, a herb known as “Ca dai hoa trang”, “Ca hoang gai”, “Ca nong”, or “Ca du” in Vietnam, is widely distributed in Vietnam. Solanum torvum has been used as folk medicine to treat various illnesses, such as stomachache, cough, fever, toothache, and bee stings. As a part of our research on genus Solanum in Vietnam, three natural compounds including paniculonin A (1), paniculonin B (2), and isorhamnetin-3-O-glucopyranoside (3) were isolated from the water layer of Solanum torvum collected in Thua Thien - Hue. This is the first time compound 3 was isolated from this species.
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