Accumulation of lipid in <i>Dunaliella salina </i>under Nutrient Starvation Condition

Abstract

The effect of nutrient starvation on lipid accumulation of Dunaliella salina A9 was studied. In nutrient starvation, cell colour changed from green to yellow (or orange) and cell growth reached stationary phase after 9 days of the culture. The study showed that under nutrient stress, decreased in cell growth is accompanied by carotenoid biosynthesis and lipid content of Dunaliella salina. The results of this study can be used to increase carotenoid and lipid production in microalgae for functional food and biofuel in the future.

Microalgal swimming signatures and neutral lipids production across growth phases

“…For all the three groups of cultures, the neutral lipid content was approximately 0.1 mg per 10 7 cells when the nitrate concentration was near the 1 mg/L. Our experimental results agree with the previous research by Gao et al (2013), Mai et al (2017), and Wang et al (2019) indicating that Dunaliella cells respond to the nitrogen starvation by rapidly transferring previously‐stored carbon to neutral lipids and enhancing lipid accumulation. The timing of peak values is also consistent with that reported in M. Chen et al (2011), which stated that the lipid content of Dunaliella cells reaches the highest value at the stationary phase in both the normal medium culture and the N‐deficient medium culture.…”

“…Researchers have investigated the microalgal lipid accumulation in response to environmental stresses induced by nutrient limitation. Nitrogen starvation has been reported to facilitate lipid accumulation in green algae cells (M. Chen et al, 2011; Gao, Yang, & Wang, 2013; Mai et al, 2017; Phadwal & Singh, 2003; Wang, Lai, Karam, de los Reyes, & Ducoste, 2019). The reported impact of nitrogen starvation on algal lipid production differs among species (Griffiths & Harrison, 2009) and is also affected by experimental design and the extent of nitrogen starvation.…”

“…in green algae cells (M. Chen et al, 2011;Gao, Yang, & Wang, 2013;Mai et al, 2017;Phadwal & Singh, 2003;Wang, Lai, Karam, de los Reyes, & Ducoste, 2019). The reported impact of nitrogen starvation on algal lipid production differs among species (Griffiths & Harrison, 2009) and is also affected by experimental design and the extent of nitrogen starvation.…”

“…,Mai et al (2017), andWang et al (2019) indicating that Dunaliella cells respond to the nitrogen starvation by rapidly transferring previously-stored carbon to neutral lipids and enhancing lipid accumulation. The timing of peak values is also consistent with that reported in M Chen et al (2011),.…”

Effect of Osmotic Stress and Nutrient Starvation on the Growth, Carotenoid and Lipid Accumulation in <i>Dunaliella salina </i>A9

“…Known salt stress-induced gene products include transcripts and proteins involved in carbon and iron assimilation as well as carotenoid biosynthesis [14,16,33]. Our previous results documented that decreased in cell growth is accompanied by carotenoid biosynthesis and lipid content of Dunaliella salina after 9 days nutrient stress [22]. …”