Rapid presumptive identification of vibrios by immobilization in distilled water

Chester, B; Poulos, E G

doi:10.1128/jcm.11.5.537-539.1980

Cited by 8 publications

(2 citation statements)

References 4 publications

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“…Using a simpler method, Chester and Poulos reported that immobilization by distilled water of pure cultures of Vibrio species and C. fetus subsp. jejuni correctly distinguished these organisms from other motile gram-negative bacteria (4). They postulated that the osmolarity of the diluent mediated this effect.…”

Section: Discussionmentioning

confidence: 95%

Dark-field microscopy of human feces for presumptive diagnosis of Campylobacter fetus subsp. jejuni enteritis

Paisley¹,

Mirrett²,

Lauer³

et al. 1982

J Clin Microbiol

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To determine the value of direct dark-field microscopy for diagnosing enteritis due to Campylobacter fetus subsp. jejuni, we examined 1,377 human fecal specimens for bacteria with typical Campylobacter darting motility, leukocytes, and erythrocytes. Eighty-four specimens (6.1%) grew C.fetus subsp. jejuni. Of the 48 specimens showing Campylobacter motility, 30 (62%) grew C. fetus subsp. jejuni. The sensitivity, specificity, and predictive value of observing Campylobacter motility were 36%, 99%, and 62%, respectively. The predictive value of detecting Campylobacter motility was improved if the specimens were diarrheal (23 of 31, 74%), leukocytes were present (25 of 33, 76%), erythrocytes were present (22 of 27, 81%), or if all of the above findings were present (18 of 20, 90%). The sensitivity of detecting Campylobacter darting motility was highest if specimens were examined within 2 h of arrival in the laboratory (15 of 30, 50%) as opposed to after 2 h (15 of 53, 28%; P less than 0.01). Prompt dark-field microscopic examination of diarrheal stool specimens is valuable for the presumptive diagnosis of Campylobacter enteritis.

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Section: Discussionmentioning

confidence: 95%

Dark-field microscopy of human feces for presumptive diagnosis of Campylobacter fetus subsp. jejuni enteritis

Paisley¹,

Mirrett²,

Lauer³

et al. 1982

J Clin Microbiol

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“…All strains were identified using criteria and procedures recommended by the American Society for Microbiology (8), the Center for Disease Control (14), and G. L. Gilardi (7). In addition, a modified silver stain for flagella (16), a semi-quantitative catalase test (3), and a distilled water immobilization test for vibrios (4) were employed.…”

Section: G L Gilardi Hospital For Joint Diseases and Medicalmentioning

confidence: 99%

Evaluation of the Minitek system for identification of nonfermentative and nonenteric fermentative Gram-negative bacteria

Chester¹,

Cleary²

1980

J Clin Microbiol

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The Minitek identification system (MT) was compared with a conventional testing battery for the characterization of 735 isolates which included 57 species and groups of nonfermentative (NF) and nonenteric fermentative (NEF) gram-negative bacteria. The MT correctly identified 585 of 616 NF (94,96%) and 115 of 119 NEF (96.65%) bacteria and 700 of 735 strains (95.24%) overall. A total of 31 NF and NEF (4.22%) bacteria were misidentified, and no identification was determined for four strains (0.69%). All strains of Acinetobacter anitratus, Pseudomonas maltophilia, P. fluroescens, and P. putida and all but one strain of P. aeruginosa were correctly identified. The most frequently misidentified taxa were CDC group Va-1, P. pickettii (Va-2), P. mendocina, and Moraxella urethralis (M-4). Supplemental tests were needed for the complete identification of 214 strains (29.11%). An average of 1.54 supplemental tests were used with each of these strains. A total of 134 strains (18.23%) had their identification delayed by 1 day due to supplemental testing. We recommend the use of the 42 degree C growth test with the MT. When used in accord with the manufacturer's instructions and with the MT code book the MT was found to be a valuable system for the identification of a wide variety of common and infrequently encountered NF and NEF bacteria.

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Characterization of Campylobacter-Like Organisms Isolated from Homosexual Men

Fennell

Totten

Quinn

et al. 1984

Journal of Infectious Diseases

172

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Thirteen Campylobacter-like organisms (CLOs) isolated from rectal cultures from homosexual men were studied. Like catalase-positive Campylobacter species, CLOs were curved gram-negative rods that did not grow aerobically, were motile, were oxidase- and catalase-positive, and did not utilize glucose. However, CLOs could not be classified within any of the Campylobacter species because they grew slowly and had unusual colony morphology; did not grow at 25 C, hydrolyze hippurate, produce H2S in triple sugar-iron agar, or tolerate 2% NaCl; were inhibited by 30-micrograms disks of nalidixic acid; and tolerated 1% glycine and 0.04% triphenyltetrazolium chloride. Three groups of CLOs were identified based on differences in nitrate reduction, growth at 42 C, and sensitivity to cephalothin. By the colony hybridization technique, whole-cell DNA isolated from a strain in each CLO group hybridized with DNA from other strains in the same group, but not with strains in other groups or with reference strains of catalase-positive Campylobacter species.

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Rapid presumptive identification of vibrios by immobilization in distilled water

Abstract: Each of 28 Vibrio strains and 3 Campylobacter strains was immobilized in distilled water (pH 6.9), but retained full motility in Trypticase soy broth. This phenomenon was not seen with any of 243 non-Vibrio, non-Campylobacter isolates representing 44 taxa of gram-negative bacteria.

Cited by 8 publications

References 4 publications

Dark-field microscopy of human feces for presumptive diagnosis of Campylobacter fetus subsp. jejuni enteritis

Dark-field microscopy of human feces for presumptive diagnosis of Campylobacter fetus subsp. jejuni enteritis

Evaluation of the Minitek system for identification of nonfermentative and nonenteric fermentative Gram-negative bacteria

Characterization of Campylobacter-Like Organisms Isolated from Homosexual Men

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