Characterization of Campylobacter-Like Organisms Isolated from Homosexual Men

Abstract

Thirteen Campylobacter-like organisms (CLOs) isolated from rectal cultures from homosexual men were studied. Like catalase-positive Campylobacter species, CLOs were curved gram-negative rods that did not grow aerobically, were motile, were oxidase- and catalase-positive, and did not utilize glucose. However, CLOs could not be classified within any of the Campylobacter species because they grew slowly and had unusual colony morphology; did not grow at 25 C, hydrolyze hippurate, produce H2S in triple sugar-iron agar, or tolerate 2% NaCl; were inhibited by 30-micrograms disks of nalidixic acid; and tolerated 1% glycine and 0.04% triphenyltetrazolium chloride. Three groups of CLOs were identified based on differences in nitrate reduction, growth at 42 C, and sensitivity to cephalothin. By the colony hybridization technique, whole-cell DNA isolated from a strain in each CLO group hybridized with DNA from other strains in the same group, but not with strains in other groups or with reference strains of catalase-positive Campylobacter species.

Isolation and characterization of a novel Helicobacter species, Helicobacter jaachi sp. nov., from common marmosets (Callithrix jaachus)

“…For example, other EHS isolated from animals and birds, such as Helicobacter bilis and Helicobacter pullorum, have been identified in patients with cholecystitis and biliary neoplasia (Fox et al, 1998;Matsukura et al, 2002;Segura-Ló pez et al, 2015). In 1984, a group of microaerobic Campylobacterlike organisms were isolated from rectal swabs of male homosexuals suffering from proctocolitis and enteritis (Fennell et al, 1984;Totten et al, 1985). Originally classified as Campylobacter cinaedi and Campylobacter fennelliae, they were later recognized to be Helicobacter spp.…”

Revision of Campylobacter, Helicobacter, and Wolinella Taxonomy: Emendation of Generic Descriptions and Proposal of Arcobacter gen. nov.

“…1). Furthermore, all four species have G + C values between 35 and 41 mol% (Table 2) (20) and have many phenotypic characteristics in common (16,20,23,59). Immunotyping (Table 4) and partial 16s rRNA sequence analysis (58) (20,23).…”

“…rRNA cluster I is linked to rRNA cluster 11 at an average Tm(c) of 65.9 5 1.6"C, and rRNA cluster I11 is linked to rRNA clusters I and I1 at an average Tnl(,) of 63.7 2 1.2"C. The structure of these rRNA clusters is shown in Fig. 1 rRNA cluster 111 consists of Wolinella succinogenes (two strains), Helicobacter pylori (two strains), Helicobacter mustelae (one strain), Helicobacter cinaedi (three strains), Helicobacter fennelliae (one strain), " Flexispira rappini" (one strain), and strain CLO-3 of Fennel1 et al (16).…”

“…, the aspartate-fermenting, free-living Campylobacter species of Laanbroek et al [27] and Spirillum sp. strain 5175 of Wolfe and Pfennig [65]), and strain CLO-3 of Fennel1 et al (16). In addition to these Campylobacter strains, we also included representative strains of Wolinella succinogenes, Wolinella curva, and Wolinella recta, and an unnamed Wolinella strain (57), as well as representative strains of Bacteroides gracilis, Bacteroides ureolyticus, and "Flexispira rappini," which is a recently described organism that has been isolated from aborted fetuses ( 5 , 26) and diarrheic stools (1).…”

Arcobacter cryaerophilusand thermophilic campylobacters in a sewage treatment plant in Italy: two secondary treatments compared

“…The bacteria are found in many habitats ranging from the purely environmental (C. nitrofigilis) to animal (C. hyointestinalis and C. cryaerophila) and human habitats. In humans the bacteria have been associated with gastro-duodenal ulcer (H. pylori), proctocolitis in homosexuals (C. cinaedi and C. fennelliae) [3][4][5][6] but mainly acute enteritis, in which C. jejuni is the most frequently detected biotype alongside C. coli, C. lari, 'C. upsaliensis' and C. hyointestinalis [7][8][9][10][11][12].…”