Evaluation of the Minitek system for identification of nonfermentative and nonenteric fermentative Gram-negative bacteria

Chester, V. E.; Cleary, Thomas P.

doi:10.1128/jcm.12.4.509-516.1980

Cited by 16 publications

(4 citation statements)

References 13 publications

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“…In addition, Townsend et al suggested that in vitro synergism between aztreonam and clavulanic acid by using disks containing 30 and 10 g of drug, respectively, can be used in the presumptive identification of the bacterium (412). Furthermore, several commercial products which are intended to aid in the identification of gram-negative non-fermentative rods have been tested for their ability to accurately identify S. maltophilia (19,70,110,205,228,229,263,271,304,332,352,356,385,387,394,407,435,441). Details of studies which have examined the utility of these methods, including automated and semiautomated systems, for the identification of this bacterium are listed in Table 4.…”

Section: Identification In the Clinical Laboratorymentioning

confidence: 99%

Microbiological and Clinical Aspects of Infection Associated with Stenotrophomonas maltophilia

1998

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SUMMARY The gram-negative bacterium Stenotrophomonas maltophilia is increasingly recognized as an important cause of nosocomial infection. Infection occurs principally, but not exclusively, in debilitated and immunosuppressed individuals. Management of S. maltophilia-associated infection is problematic because many strains of the bacterium manifest resistance to multiple antibiotics. These difficulties are compounded by methodological problems in in vitro susceptibility testing for which there are, as yet, no formal guidelines. Despite its acknowledged importance as a nosocomial pathogen, little is known of the epidemiology of S. maltophilia, and although it is considered an environmental bacterium, its sources and reservoirs are often not readily apparent. Molecular typing systems may contribute to our knowledge of the epidemiology of S. maltophilia infection, thus allowing the development of strategies to interrupt the transmission of the bacterium in the hospital setting. Even less is known of pathogenic mechanisms and putative virulence factors involved in the natural history of S. maltophilia infection and this, coupled with difficulties in distinguishing colonization from true infection, has fostered the view that the bacterium is essentially nonpathogenic. This article aims to review the current taxonomic status of S. maltophilia, and it discusses the laboratory identification of the bacterium. The epidemiology of the organism is considered with particular reference to nosocomial outbreaks, several of which have been investigated by molecular typing techniques. Risk factors for acquisition of the bacterium are also reviewed, and the ever-expanding spectrum of clinical syndromes associated with S. maltophilia is surveyed. Antimicrobial resistance mechanisms, pitfalls in in vitro susceptibility testing, and therapy of S. maltophilia infections are also discussed.

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Section: Identification In the Clinical Laboratorymentioning

confidence: 99%

Microbiological and Clinical Aspects of Infection Associated with Stenotrophomonas maltophilia

1998

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“…In this study, the Rapid NFT system accurately identified most of the nonsaccharolytic organisms tested with a mini- Alcaligenes denitrificans (1) NO3, GNT, ADI, PAC No profile A. faecalis (7) NO3, GNT, ADI A. denitrificans (5) NO3, CAP, ADI…”

Section: Discussionmentioning

confidence: 77%

“…Pseudomonas acidovorans (1) CDC group IV-e (1) ..................... Moraxella phenylpyruvica CDC group M-5 (6) ..................... Moraxella urethralis (5) No profile listed (1)…”

Section: A Denitrificansmentioning

confidence: 99%

Comparison of Rapid NFT system and conventional methods for identification of nonsaccharolytic gram-negative bacteria

Martin¹,

Siavoshi²,

McDougal³

1986

J Clin Microbiol

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“…Pseiudoitnoncas aer-laginosa, 12 Pseiudotinoncas cepacia, 6 Pseudoinonas flitorescens, 1 Pseaidotinona10s paitidai, 18 Pseiudoinonias inaltophlilia. 3 Pseiadomtionas alcaligenes, 2 Pseildotinonats pseudoalcaligenes, 3 Pseuidoinonias puitrefatciens, 4 Pseiudoinonias statzeri, 1 Pseiadoinonias paucitnobilis,5 Pseiadoinonas di,ninlta, 2 Pseludoinonats vesicitlaris, 3 Psel-doinontIais acidovorans. 2 Pseiudoinonas testoster(oni, 2 Pseldoiotnas pickettii.…”

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Collaborative evaluation of the Abbott Avantage system for identification of frequently isolated nonfermentative or oxidase-positive gram-negative bacilli

Jorgensen¹,

Dyke²,

Helgeson³

et al. 1984

J Clin Microbiol

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The capability of the Abbott Avantage system to identify 10 species of commonly isolated glucose nonfermentative or oxidase-positive gram-negative bacilli in a 5-h test period was evaluated in a collaborative study. The Avantage nonenteric data base uses 20 biochemical test reactions performed in an expanded Abbott bacterial identification cartridge plus the results of a manual oxidase test. The species included in the Avantage data base are Acinetobacter anitratus, Acinetobacter lwoffi, Aeromonas hydrophila, Flavobacterium meningosepticum-IIb group, Pseudomonas aeruginosa, Pseudomonas cepacia, Pseudomonas fluorescens-putida group, Pseudomonas maltophilia, Pasteurella multocida, and Plesiomonas shigelloides. The collaborative study included the testing of 200 coded challenge strains in all three laboratories and the subsequent testing of an additional group of 100 to 200 clinical isolates recovered independently by each laboratory. Reference identifications for all isolates were determined by conventional biochemical test reactions. The overall accuracy of identification of the coded challenge strains for the three laboratories was 97%, whereas 95% of 437 clinical isolates and selected stock cultures of clinical derivation were identified correctly.

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Evaluation of the Minitek system for identification of nonfermentative and nonenteric fermentative Gram-negative bacteria

Cited by 16 publications

References 13 publications

Microbiological and Clinical Aspects of Infection Associated with Stenotrophomonas maltophilia

Microbiological and Clinical Aspects of Infection Associated with Stenotrophomonas maltophilia

Comparison of Rapid NFT system and conventional methods for identification of nonsaccharolytic gram-negative bacteria

Collaborative evaluation of the Abbott Avantage system for identification of frequently isolated nonfermentative or oxidase-positive gram-negative bacilli

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