Rapid Multiple Immunoenzyme Assay of Mycotoxins

Urusov, Alexandr Е.; Zherdev, Anatoly V.; Petrakova, Alina V.; Садыхов, Э. Г.; Королева, О. В.; Dzantiev, Boris B.

doi:10.3390/toxins7020238

Cited by 60 publications

(26 citation statements)

References 42 publications

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“…In terms of meeting current trends, multiplexing abilities of ELISAs have been demonstrated by Urusov et al (2015). The group immobilised AFB1, OTA and ZEA in different wells of a single microplate.…”

Section: Enzyme-linked Immunosorbent Assaymentioning

confidence: 99%

Current trends in rapid tests for mycotoxins

Nolan

Auer²,

Spehar³

et al. 2019

Food Additives & Contaminants: Part A

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There is an ample number of commercial testing kits available for mycotoxin analysis on the market today, including enzyme-linked immunosorbent assays, membrane-based immunoassays, fluorescence polarisation immunoassays and fluorometric assays. It can be observed from the literature that not only are developments and improvements ongoing for these assays but there are also novel assays being developed using biosensor technology. This review focuses on both the currently available methods and recent innovative methods for mycotoxin testing. Furthermore, it highlights trends that are influencing assay developments such as multiplexing capabilities and rapid on-site analysis, indicating the possible detection methods that will shape the future market.

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Section: Enzyme-linked Immunosorbent Assaymentioning

confidence: 99%

Current trends in rapid tests for mycotoxins

Nolan

Auer²,

Spehar³

et al. 2019

Food Additives & Contaminants: Part A

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show abstract

“…Still, recoveries (87-121%), RSD r values (<17%) and the comparison of naturally contaminated cereals with LC-MS/MS derived concentrations were acceptable. Urusov et al (2015) tried to unify protocols and to shorten total analysis times for the determination of ZEA, OTA and AFB 1 with indirect competitive ELISAs. Monoclonal antibodies were biotinylated with an elongated spacer together with a streptavidin-polyperoxidase conjugate to generate signal.…”

Section: Zearalenonementioning

confidence: 99%

Developments in mycotoxin analysis: an update for 2017-2018

Tittlemier¹,

Cramer

Dall’Asta

et al. 2019

WMJ

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This review summarises developments that have been published in the period from mid-2017 to mid-2018 on the analysis of various matrices for mycotoxins. Analytical methods to determine aflatoxins, Alternaria toxins, ergot alkaloids, fumonisins, ochratoxins, patulin, trichothecenes, and zearalenone are covered in individual sections. Advances in sampling strategies are discussed in a dedicated section, as are methods used to analyse botanicals and spices, and newly developed comprehensive liquid chromatographic-mass spectrometric based multi-mycotoxin methods. This critical review aims to briefly discuss the most important recent developments and trends in mycotoxin determination as well as to address limitations of the presented methodologies.

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“…Antibodies, immobilized on a carrier, are often more stable to the denaturing influence of organic substances than free antibodies. According to the data of Urusov et al [15], when working with magnetic immunosorbents, the content of methanol in the test sample can be increased from 10 to 30%.…”

Section: Proper Sample For Lfiamentioning

confidence: 99%

Ways to Reach Lower Detection Limits of Lateral Flow Immunoassays

Zherdev¹,

Dzantiev²

2018

Rapid Test - Advances in Design, Format and Diagnostic Applications

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This chapter considers factors influencing sensitivity of lateral flow immunoassay and modern developments that are focused on reaching lower detection limits. The existing variety of proposed approaches is classified in accordance with the "big five rules" for these assays, including proper sample, receptor, interaction, response, and output. The solutions for rapid extraction of target analytes and preventing negative influence of extractants are considered. Role to antibodies affinity and specificity is characterized. Potential of alternate bioreceptor molecules is discussed. Immunoreactants' compositions, concentrations, and locations on the test strip are characterized as factors determining assay parameters. The existing variety of labels is compared in terms of their optical and alternate registration. Tools to modulate a sequence of analytical reactions and to form aggregates of the detected labels are considered. The discussed approaches are illustrated through developments of test strips for detection of mycotoxins, veterinary drugs, and other analytes.The overall design of the immunochromatographic test strip is shown in Figure 1. It is a composite of several membranes of different structures and porosities, fixed on a support. The bundling of the test strip can vary, so it makes sense to consider its design based on what analytical tasks are being performed on its different sites.A. Typically, the lower portion of the test strip contains a sample pad. It ensures the absorption of sample components, in which the presence of the target analyte is checked.B. The following is a section with immunoreagents that are washed out during the analysis and move upward along with the components of the sample. As a rule, a conjugate pad forms this zone. It contains a conjugate of antibodies against the target analyte with a nanodispersed label-particles of colored latex, colloidal gold, and so on.The next two sections are located on the main working membrane of the test strip. C.First, there is a zone along which the movement of the absorbed components of the sample and the washed immunoreagents continues. During this movement, immune reactions occur, and specific intermolecular complexes are formed. D.Next, a mixture of reacted and unreacted molecules enters the binding area with immobilized immunoreagents. Depending on whether the target analyte was present in the sample and in what amount, binding of labeled immune complexes occurs in certain areas (in the traditional case, with the formation of narrow colored lines). Usually, additional reagents are located here to control the functionality of the test system. E. The upper part of the test strip with the final pad, usually structurally similar to the sample pad, ensures the further movement of the reaction mixture under the action of capillary forces and the washing of unreacted components from the underlying areas. These processes allow the label's binding to be evaluated correctly.Membrane components of the test strip are fixed on a plastic support and partia...

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Rapid Multiple Immunoenzyme Assay of Mycotoxins

Cited by 60 publications

References 42 publications

Current trends in rapid tests for mycotoxins

Current trends in rapid tests for mycotoxins

Developments in mycotoxin analysis: an update for 2017-2018

Ways to Reach Lower Detection Limits of Lateral Flow Immunoassays

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