Current trends in rapid tests for mycotoxins

Abstract

There is an ample number of commercial testing kits available for mycotoxin analysis on the market today, including enzyme-linked immunosorbent assays, membrane-based immunoassays, fluorescence polarisation immunoassays and fluorometric assays. It can be observed from the literature that not only are developments and improvements ongoing for these assays but there are also novel assays being developed using biosensor technology. This review focuses on both the currently available methods and recent innovative methods for mycotoxin testing. Furthermore, it highlights trends that are influencing assay developments such as multiplexing capabilities and rapid on-site analysis, indicating the possible detection methods that will shape the future market.

Worldwide aflatoxin contamination of agricultural products and foods: From occurrence to control

“…When an analyte is passed through this plate or column, it is recognized by the antibodies printed on the plate or column to form a complex that then interacts with a chromogenic substrate and creates a readable signal (Bakırdere et al., 2012). There are different formats of ELISA (Aydin, 2015), however, the indirect competitive format is the most widely used for mycotoxin detection (Nolan et al., 2019).…”

Recent Advances in Mycotoxin Analysis and Detection of Mycotoxigenic Fungi in Grapes and Derived Products

“…The detection of mycotoxins is typically facilitated by the presence of a marker compound that reacts with an enzyme by non-enzymatic labels conjugated to the antibodies, or without the use of a marker, taking advantage of the natural fluorescence of some mycotoxins. A profound and detailed presentation of the different immunochemical assay principles of function, different formats, advantages, and limitations regarding different assay parameters (specificity, sensitivity, variance, cross reactivity, accuracy, precision, and measurement range), as well as their application and validation for specific mycotoxin determination in different food and feed matrices, has been reviewed in depth in previous publications [129][130][131][132]. Here a brief presentation of the different immunochemical methods and of commercially available test kits is made, with reference to their specific application to mycotoxin determination in grapes and derived products.…”

“…or biologically derived and synthetic (aptamers, recombinant antibodies, imprinted polymers, biomimetic (peptide) molecules, etc. ), or of bigger-sized biological units such as organelles, microorganisms, or tissues [129,161]. Transduces may be optical (based on surface plasmon resonance, fluorescence, optical waveguide light mode spectroscopy, and total internal reflection ellipsometry), electrochemical (impedimetric, potentiometric, amperometric), piezoelectric (quartz crystal microbalance), or thermal signal-calorimetric [162,163].…”

Advances in Colorimetric Strategies for Mycotoxins Detection: Toward Rapid Industrial Monitoring

“…Despite the current market competitiveness, the colorimetric methods dedicated to the determination of representative mycotoxins in foodstuffs continue to attract industrials for a reliable and cost-effective monitoring. Campbell et al have recently reviewed the available commercial kits [10], emphasizing that antibodybased schemes conquer the most part of the market owing to their superior specificity for real-world applications.…”