Rapid Micropropagation of Paulownia tomentosa

Abstract

Nodes from mature tissue of Paulownia tomentosa Steud. were cultured on a modified Murashige-Skoog (MS) medium containing BAP and NAA. Axillary buds elongated most rapidly from nodes of greenhouse-grown trees on a medium containing 1.0 mg BAP/liter and 0.1 mg NAA/liter. After 7 days on this medium, the elongated shoots were transferred to media containing IBA (0.5–1.0 mg/liter) or the shoots were quick-dipped (15 sec) in the K-salt of IBA (KIBA, 500–1000 ppm) and rooted under mist in the greenhouse. The shoots rooted in 7–10 days and were easily transplanted to 6 packs (6-celled plastic flat) for continued development. This propagation scheme provides a method for the rapid multiplication of plants from mature tissue Paulownia in a very short period of time (14–17 days). Chemical names used: 1-naphthaleneacetic acid (NAA); benzylaminopurine (BAP); 1H-indole-3-butanoic acid (IBA).

Growth of Paulownia ssp. Interspecific Hybrid ‘Oxytree’ Micropropagated Nursery Plants under the Influence of Plant-Growth Regulators

“…At first look, this seems unusual, as three studied retardants (daminozide, prohexadione-Ca, and trinexapac-ethyl) belong to the same group (acylcyclohexanediones). They influence the synthesis of different gibberellin forms in the same way-by blocking hydroxylation of inactive GA 20 into active GA 1 [4,29]. In addition, prohexadione-Ca (PH) and trinexapac-ethyl (TE) were applied at the same dose in the present study.…”

“…An increased demand for paulownia nursery plants to establish new plantations has been observed in Poland. Paulownia plants can be propagated generatively and vegetatively [4][5][6][7]. As paulownias are open-pollinating plants, propagation by seeds does not ensure the maintenance of the cultivar's characteristics in progeny.…”

“…PGR-plant-growth regulators; 2 0-before treatment; 3 WAT-weeks after treatment;4 different letters indicate significant differences among means based on LSD 0.05 ;5,6 values significantly higher/lower than control;7 the change relative to control; 8 SL-level of significance: ns-not significant, *** p < 0.001; 9 the F test result (SL = ns), being superior, does not allow the LSD test results to be considered as significant.…”