Micropropagation: Uses and Methods

George, Edwin F.; Hall, Michael; Klerk, G.J.M. de

doi:10.1007/978-1-4020-5005-3_2

Cited by 75 publications

(73 citation statements)

References 173 publications

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“…Micropropagation, compared with other traditional methods of propagation, has many advantages and its applications in horticulture, agriculture and forestry are spreading worldwide (Rathore et al, 2013). Micropropagation requires only a small explant for the initiation of culture (George and Debergh, 2008). In addition, the plants growing in vitro are kept in a controlled environment, which allows the effective cloning of economically important plants (Iliev et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of Calophyllum brasiliense (Cambess.) from nodal segments

et al. 2016

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Micropropagation of Calophyllum brasiliense Cambess. (Clusiaceae) is a way to overcome difficulties in achieving large-scale plant production, given the recalcitrant nature of the seeds, irregular fructification and absence of natural vegetative propagation of the species. Cultures were established using nodal segments 2 cm in length, obtained from 1-2 year old seedlings, maintained in a greenhouse. Mercury chloride and Plant Preservative Mixture™ were used in the surface sterilizing stage, better results being achieved with Plant Preservative Mixture™ incorporation in culture medium, at any concentration. Polyvinylpyrrolidone, activated charcoal, cysteine, ascorbic acid or citric acid were added to the culture medium to avoid oxidation. After 30 days of culture, polyvinylpirrolidone and ascorbic acid gave better results, eliminating oxidation in most explants. For shoot multiplication, benzylaminopurine was used in concentrations of 4.4 and 8.8 µM in Woody Plant Medium, resulting in an average of 4.43 and 4.68 shoots per explant, respectively, after 90 days. Indole-3-butyric acid and α-naphthalene acetic acid were used to induce root formation, reaching a maximum rooting rate of 24% with 20µM α-naphthalene acetic acid. For acclimatization. the rooted plants were transferred to Plantmax  substrate and cultured in a greenhouse, reaching 79% of survival after 30 days and 60% after one year.

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Section: Introductionmentioning

confidence: 99%

Micropropagation of Calophyllum brasiliense (Cambess.) from nodal segments

et al. 2016

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“…In the first stage (Stage I) of plant tissue culture, explants that are processed from ex vitro are freed from microbial contamination and transferred to an in vitro environment (George 1993, George andDebergh 2008). Explant disinfection is the first and most challenging step of establishing an aseptic culture and is therefore a prerequisite for the successful tissue culture of any plant.…”

Section: Explant Disinfection For the In Vitro Environmentmentioning

confidence: 99%

Disinfection procedures for in vitro propagation of Anthurium

Silva¹,

Winarto²,

Dobránszki

et al. 2015

Folia Horticulturae

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Disinfection of plant material is the most important step of the tissue culture protocol. In this process, an attempt is made to eliminate microbial contaminants from the surface and interior of plant material, thus giving the explant a fighting chance at survival in vitro. Initial cultures of Anthurium species and cultivars, which are usually established from ex vitro material grown in a greenhouse, pots or in the field, easily contaminate the in vitro milieu. This review highlights the differences in disinfection protocols that exist for different species or cultivars of Anthurium. The protocol needs to be adjusted based on the material used: spadices, spathes, seeds, leaves, or roots. Regrettably, most of the currently published protocols, derived from a literature that spans over 100 published papers, have numerous weaknesses and flaws in the information provided pertaining to disinfection and infection levels. Advice for future Anthurium researchers should thus be followed cautiously.Ke y wor d s: contamination, seed, sodium hypochlorite, spadix, spathe, tissue culture

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“…The use of tissue culture technology has advantages such as (George dan and de Klark, 2008): (1) The plant material used is very small; (2) small room is needed to reproduce and maintain the plant; (3) it can produce seeds from plants that slow and difficult vegetative propagated; (4) the production of seedling can be continuous throughout the year and are not dependent on climate change; (5) vegetative plant material can be stored in a long period and (6) lack of energy and space for the purpose of propagation and maintenance of plant stock.Therefore, the seed multiplication through tissue culture is to be more cost effective than traditional methods of propagation. Propagation through tissue culture is determined by many factors such as genetic factors and media including Plant Growth Regulator (PGR).…”

Section: Introductionmentioning

confidence: 99%

Seedling Propagation of Kulo Chrysanthemum by Tissue Culture

Mandang

2017

American Journal of Agricultural and Biological Sciences

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Aims of this research were to get the right dosage of Plant Growth Regulkator (PGR) and Coconut Water (CW) for shoot multiplication of Kulo chrysanthemums. The study was conducted using a factorial design of three factors namely IAA (0.0; 0.5 and 1.0 ppm), BAP (0, 0.5 and 1.0 ppm) and coconut water (0.0 and 30%). The explants used were clean micro cuttings and were cultured on Murashige and Skoog (MS) media. The media was added IAA, BAP and coconut water. The result showed that the best shoot multiplication of Kulo chrysanthemums was the MS media using combination of 0.5 ppm and 1.0 of IAA, 0.5 ppm of BAP and 30% of coconut water. Good rooting was revealed by the combination of IAA 0.5 and 1 ppm with 30% coconut water and BAP 1 ppm and 30% coconut water.

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Micropropagation: Uses and Methods

Cited by 75 publications

References 173 publications

Micropropagation of Calophyllum brasiliense (Cambess.) from nodal segments

Micropropagation of Calophyllum brasiliense (Cambess.) from nodal segments

Disinfection procedures for in vitro propagation of Anthurium

Seedling Propagation of Kulo Chrysanthemum by Tissue Culture

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