Micropropagation of Calophyllum brasiliense (Cambess.) from nodal segments

Silveira, Sheila Susy; Cordeiro-Silva, R.; Degenhardt, Juliana; Quoirin, Marguerite

doi:10.1590/1519-6984.23714

Cited by 14 publications

(12 citation statements)

References 26 publications

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“…The use of PPM® in the medium showed no effect in suppressing fungal contamination. However, Silveira et al (2016) showed that using 0.4 and 0.8% PPM in WPM culture medium eliminated bacterial contamination and the 0.8% dose reduced fungal contamination to 2% in nodal segments obtained from 1-2 year old seedlings of Calophyllum brasiliense.…”

Section: Induction and Regeneration Of Shoots In Vitromentioning

confidence: 96%

Use of micropropagation in the vegetative rescue of adult trees of Cedrela odorata L.

Santos

Costa

Rech

et al. 2021

Agraria

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The objective of this work was the vegetative rescue of Cedrela odorata L. adult trees from forest areas by micropropagation using shoot regeneration from axillary buds in vitro. Nodal segments (0.5 and 1.0 cm) of shoots formed from cuttings taken from canopy sprouts were used. The explants were successfully established in vitro on MS medium supplemented with 2.22 μM BAP, 0.49 μM AIB, 0.28 μM GA3 and PPM (0 and 8.6 μM). Shoot multiplication on MS with combinations of GA3 (0.28 and 1.12 μM) and BAP (2.2, 3.3, 4.4, 6.6, and 8.8 μM) and shoot rooting on medium with 4.9 μM AIB, 5.7 μM AIA or 5.37 μM ANA were evaluated. The MS medium supplemented with 0.28 μM GA3 and 8.8 μM BAP generated 4.16 shoots per explant. The best rooting induction was observed on medium containing 4.9 μM IBA, resulting in 60% of shoot rooting. The plantlets rooted were acclimatized and showed normal development with 97% survival rate. The use of canopy sprouts as explants is feasible in the rescue of C. odorata and the combination of BAP and GA3 favors in vitro multiplication.

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Section: Induction and Regeneration Of Shoots In Vitromentioning

confidence: 96%

Use of micropropagation in the vegetative rescue of adult trees of Cedrela odorata L.

Santos

Costa

Rech

et al. 2021

Agraria

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“…It affects key enzymes in the Krebs cycle and in the Electron Transport Chain. The ability of PPM™ to prevent microbial contaminants at culture initiation has been reported for some species as sugarcane (Digonzelli;Díaz;Carrizo de Bellone, 2005), Brassica oleracea (Rihan et al, 2012), Petunia hybrida (Miyazaki;Tan;Errington, 2010), Calophyllum brasiliense (Silveira et al, 2016), Nasturtium officinale (Faizy;Al-Zubaydi;Nair, 2017) among others.…”

Section: Introductionmentioning

confidence: 93%

Sterilization procedures and Plant Preservative Mixture on in vitro establishment of Miscanthus sinensis Andersson

Lédo

Amaral

Jenderek

et al. 2020

PCCM

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Information about the establishment phase of Miscanthus sinensis Andersson as sterile procedure to avoid the contamination were few reported. The aim of this study was to evaluate different immersion sterilization procedures with and without the biocide, Plant Preservative Mixture (PPM™) on in vitro contamination of two Miscanthus genotypes. The plant material were canes from adult plants of two accessions PI 668371 and PI 668375 cultivated in the germplasm bank of USDA-ARS, Griffin, Georgia. Apical meristems were submitted to immersion sterilization procedures (70% isopropyl alcohol and 1.5, 5.0 or 8.25% NaOCl for different time periods, followed by rinsing three times with autoclaved distilled water. After that, the explants were inoculated on MS basal medium in the presence or absence of 1 mL L -1 PPM™. The experimental design was fully randomized in a 2 x 5 x 2 factorial scheme (genotypes x sterilization process x PPM™) with five replicates per treatment and five apical meristems per experimental unit. Aseptic treatments showed no differences for percentage of bacterial and fungal contaminations and percentage of explant survival. In the presence of PPM ™ there was less bacterial contamination in the accessions (28%) than in the absence (100%). For the percentage of fungal contamination, PPM ™ had no significant effect on PI 668371 accession. However, on PI 668375 accession there was a lower percentage of fungal contamination (16%). The biocide PPM ™ may be an efficient agent to prevent bacterial contamination on in vitro cultures of Miscanthus spp and fungal contamination in PI 668375 accession.

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“…Kirika et al (2015) on Erythrina abyssinica stated that BA at 8.61 mg/l gave the highest number of microshoots. Silveira et al (2016) on Calophyllum brasiliense reported that for shoot multiplication, BA was used in concentrations of 1 and 2 ppm in Woody Plant Medium, resulting in an average of 4.43 and 4.68 shoots per explant, respectively. Sarhan et al (2017) reported that using BAP at 0.60 ppm + 1 /2 MS medium it gave the highest results of lateral buds survival %, regeneration capacity, culture efficiency, shoot number, shoot length and leaf number of Porana paniculata.…”

Section: Multiplication Stagementioning

confidence: 99%

Propagation of Dracaena Umbraculifera Plant by Tissue Culture Technique

Sayed¹

2021

Scient. J. of Flowers and Ornament. Plants

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during 2018 and 2019 seasons, with the aim to determine the best protocol of tissue culture technology to propagate Dracaena umbraculifera plant. For the possibility of spreading its cultivation to preserve it from extinction. As this plant is found in very few numbers, and it is difficult to form seeds under the climatic conditions in Egypt. Results obtained stated that using Clorox at 40% for 20 min gave the highest significant survival percentage (86.13%). For multiplication stage, benzyl adenine (BA) proved its superiority over kinetin in the production of a greater number and length of shoots. The highest number of shoots (3.20) was obtained with BA at 1.5 ppm and the highest shoots length (2.23 cm) was recorded with BA at 1.3 ppm. For rooting stage, the use of 1-naphthaleneacetic acid (NAA) at 0.3 ppm produced the highest values of roots number/plantlet (3.13) and root length (5.93 cm), while the lowest values were recorded when using indole-3-butyric acid (IBA) at 0.1 ppm. For acclimatization stage, the type of pot media had a significant effect. Using peat moss only under greenhouse condition gave the highest survival percentage (100%) and the highest values for all the measurements such as plant height (cm), number of leaves/plant, root length (cm), number of roots/plant, fresh and dry weights of leaves and roots (g/plant). Likewise, for the content of the leaves of total chlorophyll, carotenoids and the percentage of total soluble sugars. So, it can be recommended to make a protocol by using the tissue culture technique for propagating of Dracaena umbraculifera plant as follows: use Clorox at 40% for explant sterilization, using 1.5 ppm of BA in the multiplication stage, using 0.3 ppm of NAA in the rooting stage and using peat moss only to grow dracaena plant in the acclimatization stage to get plants with good growth characteristics.

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Micropropagation of Calophyllum brasiliense (Cambess.) from nodal segments

Cited by 14 publications

References 26 publications

Use of micropropagation in the vegetative rescue of adult trees of Cedrela odorata L.

Use of micropropagation in the vegetative rescue of adult trees of Cedrela odorata L.

Sterilization procedures and Plant Preservative Mixture on in vitro establishment of Miscanthus sinensis Andersson

Propagation of Dracaena Umbraculifera Plant by Tissue Culture Technique

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