Abstract:We have developed a rapid and simple indirect enzyme-labeled antibody procedure suitable for screening for antibodies to viral, bacterial, and parasitic antigens. Disposable microplates were utilized as antigen carriers and test vehicles. The test worked equally well with serum or whole unclotted blood. Using an automatic pipettor, 96 tests could be performed in 30 to 60 min. We report on the development of enzyme-labeled antibody procedures for the detection and surveillance of animal diseases.
“…Slaughter animals can quickly be screened for a multiplicity of diseases with the ELISA test (15). Automation is available, and it keeps pace with animal slaughter rates of about 600/h.…”
Hales showed how enzymes could be used instead of isotopes in an immunometric assay.The great need for early and accurate diagnosis of disease also prompted further development of immunodiagnostic methods. Radioimmunoassay Report
“…Slaughter animals can quickly be screened for a multiplicity of diseases with the ELISA test (15). Automation is available, and it keeps pace with animal slaughter rates of about 600/h.…”
Hales showed how enzymes could be used instead of isotopes in an immunometric assay.The great need for early and accurate diagnosis of disease also prompted further development of immunodiagnostic methods. Radioimmunoassay Report
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