Rapid methods for detection of bacterial resistance to antibiotics

March-Rosselló, Gabriel Alberto

doi:10.1016/j.eimce.2017.02.007

Cited by 13 publications

(17 citation statements)

References 57 publications

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“…Routine techniques in detecting antibiotic resistance are focused on the phenotypic evaluation of bacterial growth in the presence of a different set of antibiotics (March-rosselló, 2017). Isolation of pure cultures of pathogens has always been a critical part of medical microbiology (Karkman, Do, Walsh, & Virta, 2018).…”

Section: Antibiotic Susceptibility Testingmentioning

confidence: 99%

The incidence of antibiotic resistance within and beyond the agricultural ecosystem: A concern for public health

2020

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The agricultural ecosystem creates a platform for the development and dissemination of antimicrobial resistance, which is promoted by the indiscriminate use of antibiotics in the veterinary, agricultural, and medical sectors. This results in the selective pressure for the intrinsic and extrinsic development of the antimicrobial resistance phenomenon, especially within the aquaculture‐animal‐manure‐soil‐water‐plant nexus. The existence of antimicrobial resistance in the environment has been well documented in the literature. However, the possible transmission routes of antimicrobial agents, their resistance genes, and naturally selected antibiotic‐resistant bacteria within and between the various niches of the agricultural environment and humans remain poorly understood. This study, therefore, outlines an overview of the discovery and development of commonly used antibiotics; the timeline of resistance development; transmission routes of antimicrobial resistance in the agro‐ecosystem; detection methods of environmental antimicrobial resistance determinants; factors involved in the evolution and transmission of antibiotic resistance in the environment and the agro‐ecosystem; and possible ways to curtail the menace of antimicrobial resistance.

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Section: Antibiotic Susceptibility Testingmentioning

confidence: 99%

The incidence of antibiotic resistance within and beyond the agricultural ecosystem: A concern for public health

2020

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“…Rapid detection tests showed a reduction in the hospital and ICU length of stays, mortality rate, and costs in various studies [25][26][27]. Perez et al incorporated the rapid identification of pathogens through Matrix-Assisted Laser Desorption/ionization Time (MALDI-TOF) and susceptibility through BD Phoenix™, with a decrease of more than 50% in the microbiological identification times (40.6 vs. 14.5 hours; p < 0.001), adjustment to effective treatment (89.7 vs. 32 hours; p < 0.001), reduced hospital stay (23.3 vs. 15.3 days; p = 0.001) and ICU stay (16 vs. 10.7 days; p = 0.008), and 30-day mortality (21% vs. 8.9%; p = 0.01) [28].…”

Section: Discussionmentioning

confidence: 99%

“…However, antibiotic susceptibility is limited to the genes included in each panel, such as Film Array® platform (Biofire®), which only detected the presence of the KPC genes. Additionally, in some of these techniques, a decreased efficiency is observed in polymicrobial infections, thereby making the combination of additional techniques necessary [26][27][28]. On the other hand, the Bact/Alert 3D®, VITEK®, and HB&L® techniques are less costly and do not require specialized training, resulting in easy implementation in hospitals.…”

Section: Discussionmentioning

confidence: 99%

“…However, rapid techniques have recently been evaluated to detect multi-drug resistant GNB in blood culture samples [24]. In addition to molecular methods [25,26], biophysical techniques such as mass spectrometry, flow cytometry, laser nephelometry, immunochromatography, chemiluminescence, and bioluminescence are currently available [27]. The implementation of some of these techniques has shown a decrease in hospital length of stay, ICU length of stay, costs, and mortality rates [28].…”

Section: Introductionmentioning

confidence: 99%

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Rapid Phenotypic Detection of Gram-negative Bacilli Resistant to Oximinocephalosporins and Carbapenems in Positive Blood Cultures Using a Novel Protocol

Josa¹,

Bustos

Yusef

et al. 2020

Preprint

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Aim: To test a modified protocol designed to detect Gram-negative bacilli (GNB) resistant to oximinocephalosporins and carbapenems from positive blood cultures. Design: This is a prospective, cohort study of consecutive patients. Setting: A cardiovascular and University referral hospital. Patients: Patients hospitalized in a third level hospital with bacteraemia. Main variables of interest: We developed a modified protocol using HB&L® system to detect MDRP. We then attempted to determine accuracy, concordance and reduction of identification time of this novel method in a reference hospital. Descriptive statistics and logistical regressions were used. Results: Ninety-six patients with BSI were included in the study. A total of 161 positive blood cultures were analysed. Escherichia coli (50%, 81/161) was the most frequently identified pathogen followed by Klebsiella pneumoniae (15%, 24/161) and Pseudomonas aeruginosa (8%, 13/161). Thirty-two percent of isolations had usual resistance patters. However, in 34/161 (21%) of identified pathogens were producer of carbapenemasases and 21/161 (13%) of extended-spectrum β-lactamases. Concordance among our HB&L®modified protocol and traditional method was 99% (159/161). Finally, identification times were significantly shorter using our HB&L® modified protocol than traditional methods (Median [IQR]; 19 hours [18, 22] Vs 61 hours [60, 64], p<0.001). Conclusions: Here we provided novel evidence that using our HB&L® modified protocol is an effective strategy to reduce the time to MDRP detection/identification; with a great concordance rate when compared to the gold standard. Further studies are needed to confirm these findings and to determine whether this method may improve clinical outcomes.

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“…In general, after the microorganism develops a better resistance strategy, the new genes that confer resistance are disseminated between organisms of the same species or different species by means of different gene transfer strategies (March-Rosselló, 2017).…”

Section: Acceptedmentioning

confidence: 99%

An Investigation on the Heavy Metal Tolerance and Antibiotic Profile of the Pantoea agglomerans UCP1320

Acioly¹,

Vilar²,

Silveira³

et al. 2017

Int.J.Curr.Microbiol.App.Sci

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Rapid methods for detection of bacterial resistance to antibiotics

Cited by 13 publications

References 57 publications

The incidence of antibiotic resistance within and beyond the agricultural ecosystem: A concern for public health

The incidence of antibiotic resistance within and beyond the agricultural ecosystem: A concern for public health

Rapid Phenotypic Detection of Gram-negative Bacilli Resistant to Oximinocephalosporins and Carbapenems in Positive Blood Cultures Using a Novel Protocol

An Investigation on the Heavy Metal Tolerance and Antibiotic Profile of the Pantoea agglomerans UCP1320

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