Rapid method for determination of protein content in cereals and oilseeds: validation, measurement uncertainty and comparison with the Kjeldahl method

Beljkaš, Bojana; Matić, Jovana J.; Milovanović, Ivan; Jovanov, Pavle; Mišan, Aleksandra; Šarić, Ljubiša

doi:10.1007/s00769-010-0677-6

Cited by 73 publications

(24 citation statements)

References 7 publications

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“…The application of the Dumas combustion method for analyzing cereals reportedly produces satisfactorily accurate results over a long period (Beljkaš et al 2010). The repeatability and reproducibility standard deviations for analyses of cereals are lower than required by the Association of Official Analytical Chemists (Beljkaš et al 2010). Compared with the Kjeldahl method (Beljkaš et al 2010) for analyzing GPC and the chemical oxidation method (Isabella et al 2004) for analyzing GCC, the Dumas combustion method is simpler, faster, and produces fewer system errors when simultaneously analyzing the GNC, GCC, and C/N ratio of one sample.…”

Section: Discussionmentioning

confidence: 98%

“…In this study, we used an elemental analyzer based on the Dumas combustion method to rapidly and accurately determine the GNC and GCC in a large population set comprising 751 rice accessions. The application of the Dumas combustion method for analyzing cereals reportedly produces satisfactorily accurate results over a long period (Beljkaš et al 2010). The repeatability and reproducibility standard deviations for analyses of cereals are lower than required by the Association of Official Analytical Chemists (Beljkaš et al 2010).…”

Section: Discussionmentioning

confidence: 99%

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Genome-Wide Association Analysis Dissects the Genetic Basis of the Grain Carbon and Nitrogen Contents in Milled Rice

Tang

Fan

Liu

et al. 2019

Rice

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Background: Carbon (C) and nitrogen (N) are two fundamental components of starch and protein, which are important determinants of grain yield and quality. The food preferences of consumers and the expected end-use of grains in different rice-growing regions require diverse varieties that differ in terms of the grain N content (GNC) and grain C content (GCC) of milled rice. Thus, it is important that quantitative trait loci (QTLs)/genes with large effects on the variation of GNC and GCC are identified in breeding programs. Results: To dissect the genetic basis of the variation of GNC and GCC in rice, the Dumas combustion method was used to analyze 751 diverse accessions regarding the GNC, GCC, and C/N ratio of the milled grains. The GCC and GNC differed significantly among the rice subgroups, especially between Xian/Indica (XI) and Geng/Japonica (GJ). Interestingly, in the GJ subgroup, the GNC was significantly lower in modern varieties (MV) than in landraces (LAN). In the XI subgroup, the GCC was significantly higher in MV than in LAN. One, six, and nine QTLs, with 55 suggestively associated single nucleotide polymorphisms, were detected for the GNC, GCC, and C/N ratio in three panels during a single-locus genome-wide association study (GWAS). Three of these QTLs were also identified in a multi-locus GWAS. We screened 113 candidate genes in the 16 QTLs in gene-based haplotype analyses. Among these candidate genes, LOC_Os01g06240 at qNC-1.1, LOC_Os05g33300 at qCC-5.1, LOC_Os01g04360 at qCN-1.1, and LOC_Os05g43880 at qCN-5.2 may partially explain the significant differences between the LAN and MV. These candidate genes should be cloned and may be useful for molecular breeding to rapidly improve the GNC, GCC, and C/N ratio of rice. Conclusions: Our findings represent valuable information regarding the genetic basis of the GNC and GCC and may be relevant for enhancing the application of favorable haplotypes of candidate genes for the molecular breeding of new rice varieties with specific grain N and C contents.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Genome-Wide Association Analysis Dissects the Genetic Basis of the Grain Carbon and Nitrogen Contents in Milled Rice

Tang

Fan

Liu

et al. 2019

Rice

View full text Add to dashboard Cite

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“…[1][2][3][7][8][9] Among the advantages of this method are that it involves simple analytical procedures, and it does not require the use of hazardous reagents. This method sometimes gives different analytical results from those obtained by the Kjeldahl method, possibly as the result of a loss of N during the digestion and distillation stages of the Kjeldahl method.…”

Section: Introductionmentioning

confidence: 99%

“…This method sometimes gives different analytical results from those obtained by the Kjeldahl method, possibly as the result of a loss of N during the digestion and distillation stages of the Kjeldahl method. [1][2][3][7][8][9] Although further investigation is required to achieve consistency in the analytical results from the Kjeldahl and Dumas methods, [1][2][3][7][8][9] a combustion method based on the Dumas method is convenient, at least for the screening of N contents, especially when an elemental analyzer is used (<10 min per analysis). Another advantage of using an elemental analyzer is that the carbon and hydrogen contents can be determined along with the N content, and these analytical results are also useful in relation to the characterization of samples.…”

Section: Introductionmentioning

confidence: 99%

A Certified Urea Reference Material (NMIJ CRM 6006-a) as a Reliable Calibrant for the Elemental Analyses of Amino Acids and Food Samples

et al. 2014

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We examined the reliability of a certified reference material (CRM) for urea (NMIJ CRM 6006-a) as a calibrant for N, C, and H in elemental analyzers. Only the N content for this CRM is provided as an indicative value. To estimate the C and H contents of the urea CRM, we took into account the purity of the urea and the presence of other identified impurities. When we examined the use of various masses of the calibrant (0.2 to 2 mg), we unexpectedly observed low signal intensities for small masses of H and N, but these plateaued at about 2 mg. We therefore analyzed four amino acid CRMs and four food CRMs on a 2-mg scale with the urea CRM as the calibrant. For the amino acid CRMs, the differences in the analytical and theoretical contents (≤0.0026 kg/kg) were acceptable with good repeatability (≤0.0013 kg/kg in standard deviation; n = 4). For food CRMs, comparable repeatabilities to those obtained with amino acid CRMs (≤0.0025 kg/kg in standard deviation; n = 4) were obtained. The urea CRM can therefore be used as a reliable calibrant for C, H, and N in an elemental analyzer.

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“…DO (dissolved oxygen) was monitored with polarographic DO electrode (Mettler-Toledo, Greifensee, Switzerland) and controlled in 40-60% of air saturation by adjusting agitation and aeration during fermentation. based on their total nitrogen amount detected by the Kjeldahl method [17].…”

Section: Microorganism and Culture Conditionsmentioning

confidence: 99%

The dynamic regulation of nitrogen and phosphorus in the early phase of fermentation improves the erythromycin production by recombinant Saccharopolyspora erythraea strain

Zhang

Chen

Hong

et al. 2014

Bioresour. Bioprocess.

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Background: Erythromycin production often has concern with the consumption rate of amino nitrogen and phosphate, especially in the early fermentation phase. The dynamic regulation of nitrogen and phosphorus was put forward based on the comprehensive analysis of the contents of phosphorus and nitrogen in different nitrogen sources as well as the relations between nitrogen consumption and phosphorus consumption. Results: Firstly, the unstable nitrogen source, corn steep liquor, was substituted with the stable nitrogen source, yeast powder, with little effects on erythromycin production. Secondly, feeding phosphate in the early fermentation stage accelerated the consumption of amino nitrogen and ultimately increased erythromycin production by approximately 24% as compared with the control (without feeding potassium dihydrogen phosphate). Thirdly, feeding phosphate strategy successfully applied to 500 L fermenter with the final erythromycin concentration of 11839 U/mL, which was 17.3% higher than that of the control. Finally, the application of condensed soy protein (a cheap nitrogen source with low phosphorus content) combined with phosphate feed strategy led to a 13.0% increase of the erythromycin production as compared with the control (condensed soy protein, without feeding potassium dihydrogen phosphate).

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Rapid method for determination of protein content in cereals and oilseeds: validation, measurement uncertainty and comparison with the Kjeldahl method

Cited by 73 publications

References 7 publications

Genome-Wide Association Analysis Dissects the Genetic Basis of the Grain Carbon and Nitrogen Contents in Milled Rice

Genome-Wide Association Analysis Dissects the Genetic Basis of the Grain Carbon and Nitrogen Contents in Milled Rice

A Certified Urea Reference Material (NMIJ CRM 6006-a) as a Reliable Calibrant for the Elemental Analyses of Amino Acids and Food Samples

The dynamic regulation of nitrogen and phosphorus in the early phase of fermentation improves the erythromycin production by recombinant Saccharopolyspora erythraea strain

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