Rapid LC-MS/MS method for the detection of seven animal species in meat products

Zhang, Mingyue; Li, Yingying; Zhang, Yingying; Kang, Chaodi; Zhao, Wentao; Nan, Ren; Guo, Wenping; Wang, Shouwei

doi:10.1016/j.foodchem.2021.131075

Cited by 33 publications

(11 citation statements)

References 28 publications

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“…Two peptide markers for each oilseed species were used for the detection in the emulsion-type sausages. Furthermore, two heat-stable peptide markers for the meat matrix (pork 1: SALAHAVQSSR [ 55 , 56 ] and pork 2: DTLVSQLSR [ 57 ]) were established in the analytical method ( Table S4 ) as internal control standards to check the successful tryptic digestion and the correct performance of the LC-MS/MS measurement to eliminate false-negative results for the detection of the oilseed species. A chromatogram of the oilseed and meat peptide markers in an emulsion-type sausage (0.1% oilseed protein; test sausage T5; Table 1 ) is shown in Figure 5 .…”

Section: Resultsmentioning

confidence: 99%

Simultaneous Mass Spectrometric Detection of Proteins of Ten Oilseed Species in Meat Products

Spörl

Speer²,

Jira

2022

Foods

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Food fraud is a common issue in the modern food industry. The undeclared use of foreign proteins in meat products is a major concern in this context. Oilseeds are ideal for this purpose due to their high protein content and since huge amounts of oil meal are obtained as a by-product of oil production. Therefore, a UHPLC-MS/MS method was developed for the simultaneous detection of chia, coconut, flaxseed, hemp, peanut, pumpkin, rapeseed, sesame, soy, and sunflower proteins in meat products. Potential tryptic peptide markers were identified by high-resolution mass spectrometry. The final twenty peptide markers selected, which are specific for one of the ten species targeted, were each measured by multiple reaction monitoring. To the best of our knowledge, twelve new heat-stable marker peptides for chia, coconut, flaxseed, pumpkin, rapeseed, sesame and sunflower have not been reported previously. Emulsion-type sausages with 0.01, 0.25, 0.50, 0.75 and 1.00% protein addition by each oilseed species were produced for matrix calibration. No false-positive results were recorded. In the quantification of the ten oilseed species, 466 of 480 measuring data points of the recovery rate in unknown sausages (0.15 and 0.85% protein addition by each oilseed species) were in the accepted range of 80–120%.

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Section: Resultsmentioning

confidence: 99%

Simultaneous Mass Spectrometric Detection of Proteins of Ten Oilseed Species in Meat Products

Spörl

Speer²,

Jira

2022

Foods

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“…The most widely utilized multivariate analyses in certified meat products are principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), and orthogonal projections to latent structures discriminant analysis (OPLS-DA; Dailey, 2017 ). PCA is used to narrow down the list of metabolites and lipids to only the most important ones ( Zhang et al, 2022 ). Other animal species that are present in meat can be identified by PCA when validating LC-MS data ( Kang et al, 2022 ).…”

Section: Authentication Of Meat Product Using Metabolomics and Lipido...mentioning

confidence: 99%

Possibilities of Liquid Chromatography Mass Spectrometry (LC-MS)-Based Metabolomics and Lipidomics in the Authentication of Meat Products: A Mini Review

Harlina¹,

Maritha²,

Musfiroh³

et al. 2022

Food Sci Anim Resour

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The liquid chromatography mass spectrometry (LC-MS)-based metabolomic and lipidomic methodology has great sensitivity and can describe the fingerprint of metabolites and lipids in pork and beef. This approach is commonly used to identify and characterize small molecules such as metabolites and lipids, in meat products with high accuracy. Since the metabolites and lipids can be used as markers for many properties of a food, they can provide further evidence of the foods authenticity claim. Chromatography coupled to mass spectrometry is used to separate lipids and metabolites from meat samples. The research data usually is compared to lipid and metabolite databases and evaluated using multivariate statistics. LC-MS instruments directly connected to the metabolite and lipid databases software can be used to assess the authenticity of meat products. LC-MS has good selectivity and sensitivity for metabolomic and lipidomic analysis. This review highlighted the combination of metabolomics and lipidomics can be used as a reference for analyzing authentication meat products.

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“…Lipidomic is a strategy that describes comprehensive lipid profiling [ 91 ]. Lipid is one of the most important organic compounds in living organisms, whereby it functions as cell membrane defense, energy storage, and signaling molecule [ 92 ].…”

Section: Chemometrics Applications In Lipidomic Analysis For Halal Au...mentioning

confidence: 99%

The Application of Chemometrics in Metabolomic and Lipidomic Analysis Data Presentation for Halal Authentication of Meat Products

et al. 2022

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The halal status of meat products is an important factor being considered by many parties, especially Muslims. Analytical methods that have good specificity for the authentication of halal meat products are important as quality assurance to consumers. Metabolomic and lipidomic are two useful strategies in distinguishing halal and non-halal meat. Metabolomic and lipidomic analysis produce a large amount of data, thus chemometrics are needed to interpret and simplify the analytical data to ease understanding. This review explored the published literature indexed in PubMed, Scopus, and Google Scholar on the application of chemometrics as a tool in handling the large amount of data generated from metabolomic and lipidomic studies specifically in the halal authentication of meat products. The type of chemometric methods used is described and the efficiency of time in distinguishing the halal and non-halal meat products using chemometrics methods such as PCA, HCA, PLS-DA, and OPLS-DA is discussed.

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Rapid LC-MS/MS method for the detection of seven animal species in meat products

Cited by 33 publications

References 28 publications

Simultaneous Mass Spectrometric Detection of Proteins of Ten Oilseed Species in Meat Products

Simultaneous Mass Spectrometric Detection of Proteins of Ten Oilseed Species in Meat Products

Possibilities of Liquid Chromatography Mass Spectrometry (LC-MS)-Based Metabolomics and Lipidomics in the Authentication of Meat Products: A Mini Review

The Application of Chemometrics in Metabolomic and Lipidomic Analysis Data Presentation for Halal Authentication of Meat Products

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