Rapid identification of Pasteurella multocida organisms responsible for haemorrhagic septicaemia using an enzyme-linked immunosorbent assay

Dawkins, Hugh; Johnson, R.B.; Spencer, T.L.; Patten, B. E.

doi:10.1016/0034-5288(90)90056-a

Cited by 21 publications

(5 citation statements)

References 7 publications

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“…ELISA test revealed that capsular antigen is more suitable to be used in ELISA. Many researchers used differently prepared antigens to be used in the diagnosis of P. multocida , Marshall et al (1981) used sonicated whole cells of P. multocida as an antigen source, Dawkins et al (1990) identified HS-causing organisms by ELISA using a live or formalin inactivated suspension of P. multocida , Prado et al (2006) used outer membrane protein-based ELISA. Our results revealed that ELISA is more sensitive to diagnose P. multocida in apparently healthy, diseased and emergency slaughtered animals with percentages of 42%; 92.9% and 80% respectively as shown in Table 3.…”

Section: Discussionmentioning

confidence: 99%

Comparative studies for serodiagnosis of haemorrhagic septicaemia in cattle sera

El-Jakee

Ali²,

El-Shafii³

et al. 2016

Saudi Journal of Biological Sciences

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Haemorrhagic septicaemia caused by Pasteurella multocida is a major epizootic disease in cattle and buffaloes in developing countries with high morbidity and mortality rate. In the present study, a total of 88 P. multocida isolates were isolated from 256 nasopharyngeal swabs and lung tissues samples (34.4%) during the period from January, 2013 to March, 2014 from different governorates located in Egypt. Dead calves showed the highest percentage of P. multocida isolation followed by the emergency slaughtered calves, diseased calves then apparently healthy ones. These isolates were confirmed as P. multocida microscopically, biochemically by traditional tests and by API 20E commercial kit then by PCR. The percentages of positive serum samples using somatic antigen and micro-agglutination test at 1/1280 diluted serum were 10%, 54.49% and 0% in apparently healthy, diseased and emergency slaughtered samples, respectively whereas, the percentages using capsular antigen and indirect haemagglutination test were 40%, 60.89% and 60% in apparently healthy, diseased and emergency slaughtered samples, respectively. The ELISA showed the highest sensitivity for diagnosing P. multocida in apparently healthy, diseased and emergency slaughtered animals with percentages of 42%; 92.9% and 80%, respectively. The obtained results revealed that the ELISA using capsular antigen of P. multocida is a more sensitive and specific serological test for diagnosis of haemorrhagic septicaemia.

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Section: Discussionmentioning

confidence: 99%

Comparative studies for serodiagnosis of haemorrhagic septicaemia in cattle sera

El-Jakee

Ali²,

El-Shafii³

et al. 2016

Saudi Journal of Biological Sciences

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“…The antigenic analyses of P multocida strains reveal that cross‐protection is not necessarily induced by serotype‐specific capsular or somatic antigens or by major porin proteins, but by the cross‐reactive antigens (Rimler 1996). An ELISA that uses the boiled somatic antigen ‘2’ of P multocida serotype B:2 identifies haemorrhagic septicaemia‐causing serotype B:2 and E:2 organisms which contain this somatic antigen (Dawkins and others 1990). The test has also been used to detect somatic ‘2’ antibody in cattle immunised with killed haemorrhagic septicaemia vaccines (Natalia and others 1993).…”

Section: Discussionmentioning

confidence: 99%

“…The test has also been used to detect somatic ‘2’ antibody in cattle immunised with killed haemorrhagic septicaemia vaccines (Natalia and others 1993). Serotype B:3,4 is not considered to be a cause of haemorrhagic septicaemia in cattle and is ELISA test‐negative to somatic antigen ‘2’ (Dawkins and others 1990), but the identification by the same test of antibody to somatic antigen ‘2’ in cattle vaccinated with live B:3,4 vaccine (Priadi and Natalia 2001) could indicate that cross‐reactive antigens (Rimler 1996) are produced by the vaccine bacteria as they multiply in the vaccinated cattle. It is generally accepted that a killed B:2 or E:2 vaccine will not protect vaccinated ruminants against a heterologous challenge, but cross‐protection has been recorded in these animals after a natural exposure, and in cattle, buffaloes and rabbits immunised either with a live vaccine or with killed organisms which had been propagated in vivo (Bain 1964, Rimler and Boycott 1979, Bain and others 1982, Myint and others 1987, Rimler 1987).…”

Section: Discussionmentioning

confidence: 99%

Safety, efficacy and cross‐protectivity of a live intranasal aerosol haemorrhagic septicaemia vaccine

Myint¹,

Nyunt

Jones

2005

Veterinary Record

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The safety, efficacy and cross-protectivity of a live intranasal aerosol haemorrhagic septicaemia vaccine containing Pasteurella multocida serotype B:3,4 were tested in young cattle and buffaloes in Myanmar, where more than 1.5 million animals had been inoculated with this vaccine between 1989 and 1999. A recommended dose of 2 x 10(7) viable organisms was used for the efficacy test. The administration of 100 times the recommended dose to 50 cattle and 39 buffalo calves was innocuous. Seven months after they were vaccinated, three of three buffaloes were protected and 12 months after they were vaccinated, three of four buffaloes were protected against a subcutaneous challenge with serotype B:2 which killed three of three unvaccinated buffaloes. Twelve months after they were vaccinated, eight of eight cattle survived a serotype B:2 challenge, which killed four of four unvaccinated controls. The vaccinated cattle had developed serum antibodies detectable by the passive mouse protection test. Indirect haemagglutination tests on sera taken from cattle 10 days and five weeks after they were vaccinated showed high titres of antibodies. The serum of vaccinated cattle cross-protected passively immunised mice against infection with P. multocida serotypes E:2, F:3,4 and A:3,4.

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“…To identify HS-causing organisms, an ELISA test using a live or formalininactivated suspension of P. multocida was developed (Dawkins et al, 1990).…”

Section: Hsmentioning

confidence: 99%

Diagnostic and typing options for investigating diseases associated with Pasteurella multocida

Dziva

Muhairwa

Bisgaard

et al. 2008

Veterinary Microbiology

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Rapid identification of Pasteurella multocida organisms responsible for haemorrhagic septicaemia using an enzyme-linked immunosorbent assay

Cited by 21 publications

References 7 publications

Comparative studies for serodiagnosis of haemorrhagic septicaemia in cattle sera

Comparative studies for serodiagnosis of haemorrhagic septicaemia in cattle sera

Safety, efficacy and cross‐protectivity of a live intranasal aerosol haemorrhagic septicaemia vaccine

Diagnostic and typing options for investigating diseases associated with Pasteurella multocida

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