Diagnostic and typing options for investigating diseases associated with Pasteurella multocida

Dziva, Francis; Muhairwa, Amandus P.; Bisgaard, Magne; Christensen, Henrik

doi:10.1016/j.vetmic.2007.10.018

Cited by 90 publications

(84 citation statements)

References 151 publications

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“…Recently, the importance of M. hyopneumoniae was revealed when coinfection of this bacterium with PCV2 in pig herds worldwide led to increased PCVAD severity, as observed in 147 pig farms across England (Dziva et al 2008;Alarcon et al 2011). We provide the first report of M. hyopneumoniae and P. multocida in peccaries, although only one animal was positive for each agent.…”

Section: Discussionmentioning

confidence: 99%

SWINE INFECTIOUS AGENTS INTAYASSU PECARIANDPECARI TAJACUTISSUE SAMPLES FROM BRAZIL

Castro

Brombila

Bersano

et al. 2014

Journal of Wildlife Diseases

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ABSTRACT:Peccaries and pigs, Tayassuidae and Suidae respectively, diverged approximately one million years ago from a common ancestor. Because these families share some pathogens, peccaries can act as reservoirs of infectious pathogens for domestic and wild swine. We evaluated the presence of swine infectious agents in the spleen and lung tissues of white-lipped peccaries (WLP; Tayassu pecari) and collared peccaries (CP; Pecari tajacu) in Brazil. Samples from 10 adult CP and three WLP, which had been hunted by locals or hit by motor vehicles, were obtained from two free-ranging Brazilian populations. The samples were tested by PCR for Mycoplasma hyopneumoniae, Bordetella bronchiseptica, Pasteurella multocida, porcine circovirus 2 (PCV2), Suid herpesvirus 1 (SuHV-1), and porcine parvovirus (PPV). Positive samples were sequenced. Both species were negative for PPV and B. bronchiseptica and positive for PCV2 and SuHV-1. The lungs of two animals were positive for M. hyopneumoniae and P. multocida. This report is the first demonstration of PCV2 and SuHV-1 swine viruses and of M. hyopneumoniae and P. multocida bacteria in peccaries. One factor contributing to this detection was access to tissue samples, which is uncommon. The role of these infectious agents in peccaries is unknown and further epidemiologic studies should be performed. This study identified several infectious agents in peccaries and highlighted the importance of the tissue type used to detect pathogens.

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Section: Discussionmentioning

confidence: 99%

SWINE INFECTIOUS AGENTS INTAYASSU PECARIANDPECARI TAJACUTISSUE SAMPLES FROM BRAZIL

Castro

Brombila

Bersano

et al. 2014

Journal of Wildlife Diseases

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“…Most cases of FC are caused by serogroup A and, more rarely, by types F and D (Dziva et al, 2008). There is a recognized and documented association between capsule type and particular hosts and diseases .…”

Section: Identification Of the Capsule Type Of Pasteurella Multocida mentioning

confidence: 99%

“…The reduced capacity to determine the capsular type with conventional methods was also observed in other studies (Shivachandra et al, 2006;Arumugan et al, 2011). Since the development of a PCR technique for typing, this molecular method has been considered by some authors to be the gold standard technique for capsular typing, replacing phenotypic tests, particularly the passive hemagglutination test (Dziva et al, 2008). The agglutination failure of serogroups A, D and F with homologous antisera is one of the main causes of reduced sensitivity in this phenotypic test (Jabbari et al, 2006).…”

Section: Identification Of the Capsule Type Of Pasteurella Multocida mentioning

confidence: 99%

“…The agglutination failure of serogroups A, D and F with homologous antisera is one of the main causes of reduced sensitivity in this phenotypic test (Jabbari et al, 2006). Another important issue is that the passive hemagglutination test developed by Carter (1955) can be rendered ineffective by the loss of P. multocida capsule after repeated subcultures in vitro (Dziva et al, 2008). In the study by Shivachandra et al (2006) comparing both methods, 16% of 123 isolates from different avian species were not identified by conventional tests, whereas all samples were typed by multiplex PCR.…”

Section: Identification Of the Capsule Type Of Pasteurella Multocida mentioning

confidence: 99%

“…The difficulty in obtaining capsular type-specific antisera as well as the necessity of identifying and typing field isolates in the early stages of infection or prior to the development of an efficient homologous vaccine for an FC challenge are important justifications for use of the molecular method (Shivachandra et al 2006;Dziva et al, 2008). Moreover, multiplex assay protocols allow for the simultaneous detection of different genes, reducing the amount of reagents and the time required to obtain results (Perry et al, 2007).…”

Section: Identification Of the Capsule Type Of Pasteurella Multocida mentioning

confidence: 99%

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Identification of the capsule type of Pasteurella multocida isolates from cases of fowl cholera by multiplex PCR and comparison with phenotypic methods

Furian

Borges

Pilatti

et al. 2014

Rev. Bras. Cienc. Avic.

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The ability of Pasteurella multocida to invade and multiply in its host is enhanced by the presence of the capsule, one of the most important virulence factors for this bacterium. Capsular typing methods are often used in epidemiological and pathogenesis studies of this agent. Five different serogroups have been identified based on serological typing. However, such tests are laborious, and agglutination of homologous antiserum may fail. The aim of this study was to develop a multiplex PCR protocol for the identification of the hyaD-hyaC and dcbF genes specific to serogroups A and D, respectively, and to compare these results with those of phenotypic tests for 54 strains isolated from fowl cholera cases in southern Brazil. The kappa coefficient and chisquare statistics were calculated to assess the agreement between the diagnostic methods and to determine the significance of the results, respectively. The multiplex PCR was able to detect the evaluated genes. Forty-nine strains (90.74%) were classified into serogroup A, and only two isolates (3.7%) were not identified as belonging to any of the serogroups analyzed. In contrast, with the phenotypic tests, only 41 strains (75.93%) were classified into serogroup A and 11 samples (20.37%) were unidentifiable. Of the strains analyzed, 70.37% were classified into the same serogroup (A) by both methods, and the kappa coefficient (k = 0.017) indicated poor agreement between the tests. Thus, multiplex PCR is an alternative for P. multocida capsular typing, as it allows the simultaneous and rapid detection of genes and also provides a greater strain-typing capacity.

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Pasteurellosis

Brockmeier²

2019

Diseases of Swine

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Diagnostic and typing options for investigating diseases associated with Pasteurella multocida

Cited by 90 publications

References 151 publications

SWINE INFECTIOUS AGENTS INTAYASSU PECARIANDPECARI TAJACUTISSUE SAMPLES FROM BRAZIL

SWINE INFECTIOUS AGENTS INTAYASSU PECARIANDPECARI TAJACUTISSUE SAMPLES FROM BRAZIL

Identification of the capsule type of Pasteurella multocida isolates from cases of fowl cholera by multiplex PCR and comparison with phenotypic methods

Pasteurellosis

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