Rapid Identification of
            <i>Candida glabrata</i>
            with a New Commercial Test, GLABRATA RTT

Freydière, Anne-Marie; Robert, Raymond; Ploton, C.; Marot-Leblond, A.; Monerau, F.; Vandenesch, François

doi:10.1128/jcm.41.8.3861-3863.2003

Cited by 23 publications

(12 citation statements)

References 24 publications

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“…The results of this study show consistently high sensitivity and specificity from one laboratory to another, and this confirms the good interlaboratory reproducibility previously found between two laboratories (8). The sensitivity and specificity also compare well with those achieved with slower trehalasebased tests (3,10,13).…”

supporting

confidence: 76%

“…Previously evaluated with 330 stock isolates subcultured on three chromogenic media and Sabouraud-chloramphenicol agar, GLABRATA RTT had sensitivity and specificity rates for C. glabrata in excess of 94%, regardless of the isolation medium (8). In this study, GLABRATA RTT was capable of identifying fresh C. glabrata isolates with a sensitivity of 95.8% and a specificity of 98.9%.…”

mentioning

confidence: 71%

“…It is based on the ability of C. glabrata to hydrolyze trehalose and includes both a maltose test and a control test to improve the specificity of the test as previously recommended (7,15). In a preliminary study with 330 stock isolates, GLABRATA RTT reliably identified C. glabrata subcultured on three commonly used chromogenic media: Candida ID, CandiSelect, and CHROMagar Candida (8). The present evaluation was designed to determine the utility of GLABRATA RTT for identification of fresh C. glabrata isolates from primary isolation media.…”

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confidence: 99%

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Routine Use of a Commercial Test, GLABRATA RTT, for Rapid Identification of Candida glabrata in Six Laboratories

et al. 2004

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When evaluated in six clinical laboratories from six countries with 1,174 fresh isolates, including 715 Candida glabrata and 459 non-C. glabrata strains, GLABRATA RTT (Fumouze Diagnostics, Levallois Perret, France) yielded an overall sensitivity and an overall specificity of 95.8 and 98.9%, respectively. The results were consistent from one laboratory to another. The five false-positive results corresponded to C. parapsilosis (n ‫؍‬ 2), C. tropicalis, C. guilliermondii, and C. lusitaniae. GLABRATA RTT allows a rapid, cost-effective, and reliable presumptive identification of C. glabrata.

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confidence: 76%

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confidence: 71%

mentioning

confidence: 99%

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Routine Use of a Commercial Test, GLABRATA RTT, for Rapid Identification of Candida glabrata in Six Laboratories

et al. 2004

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“…The clinical specimens were cultured on Candida ID (bioMérieux, Marcy l'Etoile, France) at 37°C for 48 h. This chromogenic medium is intended to rapidly discriminate between C. albicans and non-albicans species (31). All blue colonies were identified as C. albicans, and white colonies were phenotyped by using the commercial ATB ID 32C test (API; bioMérieux, Marcy l'Etoile, France) and the Glabrata RTT test (Fumouze Diagnostic, Paris France) (11). The white colonies identified as C. glabrata, one for each specimen, were harvested into plastic tubes and stored at Ϫ80°C until further processing.…”

Section: Methodsmentioning

confidence: 99%

Microsatellite Marker Analysis as a Typing System for Candida glabrata

et al. 2005

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Candida glabrata is one of the most important causes of nosocomial fungal infection. We investigated, using a multiplex PCR, three polymorphic microsatellite markers, RPM2, MTI, and ERG3, in order to obtain a rapid genotyping method for C. glabrata. One set of primers was designed for each locus, and one primer of each set was dye labeled to read PCR signals using an automatic sequencer. Eight reference strains including other Candida species and 138 independent C. glabrata clinical isolates were tested. The clinical isolates were collected from different anatomical sites of adult patients either hospitalized in different wards of two different hospitals or not hospitalized. Since C. glabrata is haploid, one single PCR product for each PCR set was obtained and assigned to an allele. The numbers of different alleles were 5, 7, and 15 for the RPM2, MTI, and ERG3 loci, respectively. The number of allelic associations was 21, leading to a discriminatory power of 0.84. The markers were stable after 25 subcultures, and the amplifications were specific for C. glabrata. A factorial correspondence analysis did not indicate any correlation between the 21 multilocus genotypes and the clinical data (source, sex, ward, anatomical sites). Microsatellite marker analysis is a rapid and reliable technique to investigate clinical issues concerning C. glabrata. However, its discriminatory power should be improved by testing other polymorphic microsatellite loci.Epidemiological surveillance of candidemia shows that although Candida albicans remains the main species, Candida glabrata is the second leading species recovered from blood cultures in Europe and in the United States (20, 28), especially in intensive care units (9). The same phenomenon is observed for recurrent vaginitis (25). Concern arises about this increase in the incidence of C. glabrata infections because of its frequent decreased sensitivity to azoles compared to C. albicans (14,22) and the high mortality rate of bloodstream infections (28,29).Investigations for C. glabrata have not been as extensive as for C. albicans. The epidemiology is thought to be similar to C. albicans (10). However, few tools are available to study the source of contamination, cross-contamination of patients at risk, and the biology of this yeast, which has, unlike C. albicans, a haploid genome (10). Studies of population structure have begun to emerge using enzymatic and randomly amplified polymorphic DNA typing (6) or sequencing of the cytochrome c oxidase subunit 2 gene (24). Recently, a technique based on the sequencing of six variable-locus genes, or multilocus sequence typing (MLST), has been proposed as a powerful typing method (7). Microsatellites represent another class of genetic markers, defined as short tandem repeats of two to six nucleotides, known to be highly polymorphic (30). Primarily developed for human genetics, they are used for fungi (15) and more specifically for pathogenic fungi such as C. albicans (3-5, 19, 23, 26) and Aspergillus fumigatus (1, 2, 16, 17).We descri...

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“…Due to the commonly occurring innate or acquired resistance of C. glabrata to fluconazole, rapid identification is essential. Therefore, several groups have tried to establish protocols for screening and rapid identification of this yeast (3)(4)(5)(6)(7)(8)(9)(10).…”

mentioning

confidence: 99%

Comparison of a New Commercial Test, GLABRATA RTT, with a Dipstick Test for Rapid Identification of Candida glabrata

et al. 2005

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Rapid Identification of Candida glabrata with a New Commercial Test, GLABRATA RTT

Cited by 23 publications

References 24 publications

Routine Use of a Commercial Test, GLABRATA RTT, for Rapid Identification of Candida glabrata in Six Laboratories

Routine Use of a Commercial Test, GLABRATA RTT, for Rapid Identification of Candida glabrata in Six Laboratories

Microsatellite Marker Analysis as a Typing System for Candida glabrata

Comparison of a New Commercial Test, GLABRATA RTT, with a Dipstick Test for Rapid Identification of Candida glabrata

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