Routine Use of a Commercial Test, GLABRATA RTT, for Rapid Identification of
 Candida glabrata
 in Six Laboratories

Freydière, Anne-Marie; Perry, John D.; Faure, O.; Willinger, Birgit; Tortorano, Anna Maria; Nicholson, A.; Pemán, J; Verweij, Paul E.

doi:10.1128/jcm.42.10.4870-4872.2004

Cited by 21 publications

(9 citation statements)

References 23 publications

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“…parapsilosis, C. glabrata/C. krusei, and C. tropicalis but does not select for each species (www.advandx.com), while the RTT selectively identifies only C. glabrata (6).…”

Section: Discussionmentioning

confidence: 99%

Comparison of Whole Blood, Serum, and Plasma for Early Detection of Candidemia by Multiplex-Tandem PCR

et al. 2010

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We applied multiplex-tandem PCR (MT-PCR) to 255 EDTA whole-blood specimens, 29 serum specimens, and 24 plasma specimens from 109 patients with Candida bloodstream infection (candidemia) to determine whether a diagnosis could be expedited in comparison with the time to diagnosis by the use of standard blood culture. Overall, the MT-PCR performed better than blood culture with DNA extracted from whole blood from 52/74 (70%) patients, accelerating the time to detection (blood culture flagging) and determination of the pathogenic species (by use of the API 32C system [bioMérieux, Marcy l'Etoile, France]) by up to 4 days (mean, 2.2 days; range, 0.5 to 8 days). Candida DNA was detected more often in serum (71%) and plasma (75%) than in whole blood (54%), although relatively small numbers of serum and plasma specimens were tested. The sensitivity, specificity, positive predictive value, and negative predictive value of the assay with whole blood were 75%, 97%, 95%, and 85%, respectively. Fungal DNA was not detected by MT-PCR in 6/24 (25%) wholeblood samples drawn simultaneously with the positive blood culture sample. MT-PCR performed better with whole-blood specimens stored at ؊20°C (75%) and when DNA was extracted within 1 week of sampling (66%). The molecular and culture identification results correlated for 61 of 66 patients (92%); one discrepant result was due to misidentification by culture. All but one sample from 53 patients who were at high risk of candidemia but did not have proven disease were negative by MT-PCR. The results demonstrate the good potential of MT-PCR to detect candidemia, to provide Candida species identification prior to blood culture positivity, and to provide improved sensitivity when applied to with serum and plasma specimens.

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“…parapsilosis, C. glabrata/C. krusei, and C. tropicalis but does not select for each species (www.advandx.com), while the RTT selectively identifies only C. glabrata (6).…”

Section: Discussionmentioning

confidence: 99%

Comparison of Whole Blood, Serum, and Plasma for Early Detection of Candidemia by Multiplex-Tandem PCR

et al. 2010

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“…This property of C. glabrata was subsequently recognized as a rapid and cost-effective means to identify this pathogen (Land et al 1996;Fenn et al 1999;Lopez et al 2001;Soussi-Abdallaoui et al 2003;Freydiere et al 2003Freydiere et al , 2004Murray et al 2005;Willinger et al 2005;Fraser et al 2012). These rapid diagnostic tests could theoretically impact patient care by affecting therapy selection, especially in cases of candidemia.…”

Section: Introductionmentioning

confidence: 99%

Secretion of the acid trehalase encoded by the CgATH1 gene allows trehalose fermentation by Candida glabrata

Zilli

Lopes

Alves

et al. 2015

Microbiological Research

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The emergent pathogen Candida glabrata differs from other yeasts because it assimilates only two sugars, glucose and the disaccharide trehalose. Since rapid identification tests are based on the ability of this yeast to rapidly hydrolyze trehalose, in this work a biochemical and molecular characterization of trehalose catabolism by this yeast was performed. Our results show that C. glabrata consumes and ferments trehalose, with parameters similar to those observed during glucose fermentation. The presence of glucose in the medium during exponential growth on trehalose revealed extracellular hydrolysis of the sugar by a cell surface acid trehalase with a pH optimum of 4.4. Approximately ∼30% of the total enzymatic activity is secreted into the medium during growth on trehalose or glycerol. The secreted enzyme shows an apparent molecular mass of 275 kDa in its native form, but denaturant gel electrophoresis revealed a protein with ∼130 kDa, which due to its migration pattern and strong binding to concanavalin A, indicates that it is probably a dimeric glycoprotein. The secreted acid trehalase shows high affinity and activity for trehalose, with Km and Vmax values of 3.4 mM and 80 U (mg protein)(-1), respectively. Cloning of the CgATH1 gene (CAGLOK05137g) from de C. glabrata genome, a gene showing high homology to fungal acid trehalases, allowed trehalose fermentation after heterologous expression in Saccharomyces cerevisiae.

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“…Although neither C. albicans nor C. dubliniensis has high rates of antifungal drug resistance among isolates derived from blood or other body sites, the rate of azole resistance among oral isolates of C. dubliniensis from HIV-infected patients is much greater [5,6,7,8]. Easy to use, rapid assimilation methods have been developed for the differentiation of Candida glabrata from other Candida species, for instance, the GLABRATA RTT test [25] (Fumouze Diagnostics, France) and the rapid-assimilation-of-trehalose test [26]. Hence, it would be of much interest if investigators could try to develop a similar rapid assimilation method using XYL.…”

Section: Discussionmentioning

confidence: 99%

Rapid Differentiation of Candida dubliniensis from Candida albicans by Early D-Xylose Assimilation

Ellepola

Khan

2012

Med Princ Pract

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Objective: To determine if D-xylose (XYL) and/or α-methyl-D-glucoside (MDG) assimilation can be used reliably as a rapid test to differentiate Candida dubliniensis from Candida albicans at an earlier time point such as 2 h after inoculation. Materials and Methods: Thirty isolates of C. albicans and C. dubliniensis recovered from anatomical sites and clinical specimens were used. Isolates were inoculated into the API 20C AUX yeast identification system, and incubated at 30°C. XYL and MDG assimilations were read at 2-hour intervals beginning 2 h after the initial inoculation and up to 24 h of incubation; thereafter, results were read after 48 and 72 h. Results: Twenty-nine (97%) C. albicans isolates had assimilated XYL at 16 h and, by 24 h, all isolates were positive for XYL assimilation. None of the C. dubliniensis isolates assimilated XYL. The MDG assimilation revealed that 24, 40, 92 and 100% of C. albicans isolates became positive after 16, 24, 48 and 72 h of incubation, respectively, whereas only 3% of C. dubliniensis isolates assimilated MDG after 72 h. Conclusions: The findings showed that it is possible to rapidly differentiate C. albicans from C. dubliniensis isolates using the API 20C AUX carbohydrate assimilation kits after 16 h of incubation at 30°C based on the XYL assimilation.

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Routine Use of a Commercial Test, GLABRATA RTT, for Rapid Identification of Candida glabrata in Six Laboratories

Cited by 21 publications

References 23 publications

Comparison of Whole Blood, Serum, and Plasma for Early Detection of Candidemia by Multiplex-Tandem PCR

Comparison of Whole Blood, Serum, and Plasma for Early Detection of Candidemia by Multiplex-Tandem PCR

Secretion of the acid trehalase encoded by the CgATH1 gene allows trehalose fermentation by Candida glabrata

Rapid Differentiation of <b><i>Candida dubliniensis</i></b> from <b><i>Candida albicans</i></b> by Early <i>D</i>-Xylose Assimilation

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