Comparison of a New Commercial Test, GLABRATA RTT, with a Dipstick Test for Rapid Identification of
            <i>Candida glabrata</i>

Willinger, Birgit; Wein, Susanne; Hirschl, Alexander M.; Rotter, M.; Manafi, M.

doi:10.1128/jcm.43.1.499-501.2005

Cited by 18 publications

(10 citation statements)

References 10 publications

(6 reference statements)

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“…and resulting modification of antifungal therapy (37). Another major advantage cited by all current studies is the rare incidence of incorrect identification reported when the identification score was within the "acceptable" category (17,20,21,24). Isolates that generated poor-quality spectra and species not present in the reference library received low identification scores and were categorized as "unacceptable identification."…”

Section: Resultsmentioning

confidence: 97%

“…However, not all C. albicans isolates produce germ tubes, and other Candida species, most notably C. tropicalis and C. dublinensis, can also form similar structures that may be misinterpreted as germ tubes (19). Similarly, a positive rapid assimilation of trehalose (RAT) test can presumptively identify C. glabrata, but several species, including C. tropicalis, can produce false-positive results if the test is performed incorrectly (20). Because of these limitations, rapid tests often must be confirmed using additional methods.…”

Section: Current Methods For the Identification Of Yeasts And Yeast-lmentioning

confidence: 99%

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Advances in Identification of Clinical Yeast Isolates by Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Buchan

Ledeboer

2013

J Clin Microbiol

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Matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS)-based identification is being adopted by clinical laboratories for routine identification of microorganisms. To date, the majority of studies have focused on the performance and optimization of MALDI-TOF MS for the identification of bacterial isolates. We review recent literature describing the use of MALDI-TOF MS for the routine identification of a variety of yeasts and yeast-like isolates. Specific topics include the effect of optimized or streamlined extraction methods, modified scoring thresholds, expanded reference libraries, and the possibility of conducting antifungal susceptibility testing using MALDI-TOF MS.

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Section: Resultsmentioning

confidence: 97%

Section: Current Methods For the Identification Of Yeasts And Yeast-lmentioning

confidence: 99%

Advances in Identification of Clinical Yeast Isolates by Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Buchan

Ledeboer

2013

J Clin Microbiol

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“…This property of C. glabrata was subsequently recognized as a rapid and cost-effective means to identify this pathogen (Land et al 1996;Fenn et al 1999;Lopez et al 2001;Soussi-Abdallaoui et al 2003;Freydiere et al 2003Freydiere et al , 2004Murray et al 2005;Willinger et al 2005;Fraser et al 2012). These rapid diagnostic tests could theoretically impact patient care by affecting therapy selection, especially in cases of candidemia.…”

Section: Introductionmentioning

confidence: 99%

Secretion of the acid trehalase encoded by the CgATH1 gene allows trehalose fermentation by Candida glabrata

Zilli

Lopes

Alves

et al. 2015

Microbiological Research

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The emergent pathogen Candida glabrata differs from other yeasts because it assimilates only two sugars, glucose and the disaccharide trehalose. Since rapid identification tests are based on the ability of this yeast to rapidly hydrolyze trehalose, in this work a biochemical and molecular characterization of trehalose catabolism by this yeast was performed. Our results show that C. glabrata consumes and ferments trehalose, with parameters similar to those observed during glucose fermentation. The presence of glucose in the medium during exponential growth on trehalose revealed extracellular hydrolysis of the sugar by a cell surface acid trehalase with a pH optimum of 4.4. Approximately ∼30% of the total enzymatic activity is secreted into the medium during growth on trehalose or glycerol. The secreted enzyme shows an apparent molecular mass of 275 kDa in its native form, but denaturant gel electrophoresis revealed a protein with ∼130 kDa, which due to its migration pattern and strong binding to concanavalin A, indicates that it is probably a dimeric glycoprotein. The secreted acid trehalase shows high affinity and activity for trehalose, with Km and Vmax values of 3.4 mM and 80 U (mg protein)(-1), respectively. Cloning of the CgATH1 gene (CAGLOK05137g) from de C. glabrata genome, a gene showing high homology to fungal acid trehalases, allowed trehalose fermentation after heterologous expression in Saccharomyces cerevisiae.

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“…Latex agglutination kits are now commercially available and allow a species identification of C. albicans , Candida dubliniensis and C. krusei within minutes (Table 1) [43–45]. C. glabrata can be rapidly identified because of its high amounts of preformed intracellular trehalase enzyme [43,45–48]. Finally, peptide nucleotide analogue probes and fluorescence microscopy can be used [27,29,30].…”

Section: Mycological Diagnosticsmentioning

confidence: 99%

Invasive fungal infections in the paediatric and neonatal population: diagnostics and management issues

Arendrup

Fisher

Zaoutis

2009

Clinical Microbiology and Infection

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Invasive fungal infections in children appear to have increased over the past few decades. Especially neonates and children with primary and secondary immunodeficiencies are at risk. Candida and Aspergillus spp. are the most commonly isolated organisms. In addition, Malassezia may cause systemic infections in newborns and zygomycosis is important because of its rising incidence and high case fatality rate. Timely diagnosis and initiation of appropriate antifungal therapy is imperative for improving outcomes. However, traditional techniques are time-consuming and representative sample material, using invasive procedures, may be difficult to obtain in the paediatric setting. This review provides an overview of the advances in detection and rapid species identification, with a focus on issues relevant in these settings. Subsequently, the current antifungal treatment options for neonates and children are discussed in light of the antifungal spectrum of the available agents and the specific pharmacokinetic properties in different age groups. Although a multitude of newer antifungal compounds have become available within the last decade, further studies are necessary to clearly establish the role for each of these agents among neonates and children.

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Comparison of a New Commercial Test, GLABRATA RTT, with a Dipstick Test for Rapid Identification of Candida glabrata

Cited by 18 publications

References 10 publications

Advances in Identification of Clinical Yeast Isolates by Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Advances in Identification of Clinical Yeast Isolates by Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Secretion of the acid trehalase encoded by the CgATH1 gene allows trehalose fermentation by Candida glabrata

Invasive fungal infections in the paediatric and neonatal population: diagnostics and management issues

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