Advances in Identification of Clinical Yeast Isolates by Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Buchan, Blake W.; Ledeboer, Nathan A.

doi:10.1128/jcm.03105-12

Cited by 81 publications

(47 citation statements)

References 49 publications

(90 reference statements)

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“…MALDI-TOF MS has been applied successfully to identify Cryptococcus species, including varieties and hybrids, Candida albicans and non-albicans Candida species, and rare pathogenic yeasts (29,30,(38)(39)(40)(41)(42)(43)(44). Non-Candida species isolates, such as Geotrichum, Galactomyces, Saprochaete, Magnusiomyces, and Trichosporon spp., are underrepresented in the reference library and thus MALDI-TOF MS identification runs were less successful, resulting in low identification rates or failure of reliable analysis (36,45). Our data showed a high level of discriminatory power for MALDI-TOF MS, providing reliable identification of both ascomycetous and basidiomycetous arthroconidial yeasts.…”

Section: Discussionmentioning

confidence: 80%

Identification of Medically Relevant Species of Arthroconidial Yeasts by Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

et al. 2013

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Section: Discussionmentioning

confidence: 80%

Identification of Medically Relevant Species of Arthroconidial Yeasts by Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

et al. 2013

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“…Several studies have reported that matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is an accurate, rapid, and inexpensive method for identification of clinically relevant yeasts (10,11). In our previous study, we evaluated the performance of the Vitek MS system (bioMérieux, France) for identifying yeast isolates collected as part of the CHIF-NET program in 2011 (12).…”

mentioning

confidence: 99%

A Comprehensive Evaluation of the Bruker Biotyper MS and Vitek MS Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems for Identification of Yeasts, Part of the National China Hospital Invasive Fungal Surveillance Net (CHIF-NET) Study, 2012 to 2013

Fan

Kudinha

et al. 2016

J Clin Microbiol

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Invasive fungal infections are associated with high mortality and morbidity rates, especially in immunocompromised and critically ill patients (1-3). Although the most important causes of opportunistic mycoses are Candida species, especially Candida albicans, the incidence of invasive fungal infections due to nonalbicans Candida species is increasing, especially in China (4-8).The National China Hospital Invasive Fungal Surveillance Net (CHIF-NET) program is a nationwide, multicenter surveillance network established in July 2009 to provide updated information on the epidemiology of invasive fungal infections in China (9). The continual expansion of the CHIF-NET program has led to a dramatic increase in the number of laboratories submitting isolates. Therefore, an accurate, time-saving, cost-effective, and userfriendly yeast identification method is needed to replace the highcost and labor-intensive sequence-based methods used by the central laboratory.Several studies have reported that matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is an accurate, rapid, and inexpensive method for identification of clinically relevant yeasts (10, 11). In our previous study, we evaluated the performance of the Vitek MS system (bioMérieux, France) for identifying yeast isolates collected as part of the CHIF-NET program in 2011 (12). In the present study, we further systematically compared the performance of the Bruker Biotyper MS (Bruker Daltonics GmbH, Germany) and the Vitek MS systems for the identification of a large number of clinically relevant yeast isolates collected through the CHIF-NET program (2012 to 2013).(This study was presented in part at the 19th Congress of the International Society for Human and Animal Mycology (ISHAM), Melbourne, Australia, 2015.)The 2,683 yeast isolates analyzed in this study were all derived from patients with invasive candidiasis from 48 clinical microbiology laboratories of hospitals situated in 24 provinces across China during the period August 2012 to July 2013. The majority of the nonduplicate isolates were obtained from blood (44.2%), followed by ascitic fluid (18.2%), intravascular catheters (7.6%), pus (7.3%), and cerebrospinal fluid (5.9%) (see Table S1 in the supplemental material). The isolates were inoculated onto CHROMagar Candida medium (CHROMagar, Paris, France), incubated for 48 h at 35°C, and then simultaneously identified using the Vitek MS and Bruker Biotyper MS systems.For the Vitek MS v.2.0 system, proteins were extracted as recommended by the manufacturer. Briefly, a small portion of a single colony was directly spotted onto a target plate and covered with 0.5 l of formic acid (bioMérieux). All mass profiles were analyzed using the Vitek MS database (MS-ID v.2.0 knowledge base clinical use). For the Bruker Biotyper MS system, pure yeast isolates (each from a single colony), were directly smeared onto the target plate (Bruker Daltonics GmbH) and overlaid with 1 l of 70% formic acid (Sigma-Aldrich). Each spectrum was analyzed by ...

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“…Mayaların MALDI-TOF MS ile identifikasyonu öncesinde yaklaşık 25 dakika süren bir ekstraksiyon aşaması gerçekleştirilmesi gerekli olmakla birlikte, bazı araştırmacılar buna alternatif olan ve daha kısa süren yöntemler geliştirdiklerini bildirmişlerdir (13)(14)(15)(16)(17)(18)(19) .…”

Section: Discussionunclassified

Comparision of matrix-assisted laser desorption ionization-time of flight (MALDI-TOF MS) with API ID 32C and VITEK 2 for the identification of Trichosporon species

Pelit¹,

Genç²,

Barış³

et al. 2017

TMCD

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ÖZ Amaç Anahtar kelimeler: Trichosporon, identifikasyon, MALDİ TOF MS ABSTRACT Comparision of Matrix-Assisted Laser Desorption Ionizationtime of Flight (MALDI-TOF MS) with API ID 32C and VITEK 2 For the Identification of Trichosporon Species Objective: Trichosporon species have a widespread distribution in the nature and human skin and mucosa can be colonized with Trichosporon species. Invasive infections caused by Trichosporon species are uncommon but frequently fatal in immunosuppressive patients. Correct identification of Trichosporon species is challenging but important for appropriate treatment approches. As an alternative to phenotypic and genome-based identification techniques, Matrix-assisted laser desorption ionizationtime of flight (MALDI-TOF) mass spectrometry (MS) is emerging as a rapid and accurate identification method. In this study we aimed to evaluate MALDI-TOF MS (Bruker Daltonics, Bremen, Germany)

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Advances in Identification of Clinical Yeast Isolates by Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Cited by 81 publications

References 49 publications

Identification of Medically Relevant Species of Arthroconidial Yeasts by Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

Identification of Medically Relevant Species of Arthroconidial Yeasts by Use of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

A Comprehensive Evaluation of the Bruker Biotyper MS and Vitek MS Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems for Identification of Yeasts, Part of the National China Hospital Invasive Fungal Surveillance Net (CHIF-NET) Study, 2012 to 2013

Comparision of matrix-assisted laser desorption ionization-time of flight (MALDI-TOF MS) with API ID 32C and VITEK 2 for the identification of Trichosporon species

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