Rapid Identification of Candida dubliniensis by Indirect Immunofluorescence Based on Differential Localization of Antigens on C. dubliniensis Blastospores and Candida albicans Germ Tubes

Abstract

There is a clear need for the development of a rapid and reliable test for the identification of Candida dubliniensis and for the discrimination of this species from Candida albicans. In the present study we have investigated the potential use of C. dubliniensis-specific antigens as a basis for its identification. We produced an anti-C. dubliniensis serum which, after adsorption with C. albicans blastospores, was found to differentially label C. dubliniensis isolates in an indirect immunofluorescence test. In this test, the antiserum reacted with blastospores and germ tubes of C. dubliniensis and with blastospores of Candida krusei and Rhodotorula rubra but did not react with blastospores of several otherCandida species including C. albicans. The antiserum also reacted with C. albicans germ tubes. The anti-C. dubliniensis adsorbed serum reacted with specific components of 25, 28, 37, 40, 52, and 62 kDa in the C. dubliniensis extract and with a variety of antigens from other yeast species. The antigens from non-C. dubliniensis yeasts showing reactivity with the anti-C. dubliniensis adsorbed serum are mostly expressed within the cell walls of these yeast species, and this reactivity does not interfere with the use of the anti-C. dubliniensis adsorbed serum in an indirect immunofluorescence test for the rapid identification of C. dubliniensis.

Comparative evaluation of three commercial software packages for analysis of DNA polymorphism patterns

“…The isolates, including 12 C. albicans from 11 patients and six C. dubliniensis from four patients, were identified by conventional mycological identification methods and by their carbohydrate assimilation patterns on ID 32C strips (bioMérieux, Marcyl'Etoile, France). C. dubliniensis isolates identities were confirmed by immunofluorescence as previously described [11,12]. Polymerase chain reactions were performed according to the method described by Alonso et al [13], using the primer AB1-12 (5 0 -CCTTGACGCA-3 0 ) purchased from Advanced Technologies Ltd. (Leatherhead, Surrey, UK).…”

The Impact of Oral Contraceptives on Women's Periodontal Health and the Subgingival Occurrence of Aggressive Periodontopathogens and Candida Species

“…Definitive identification of C. dubliniensis was done by means of their colony color and morphology in medium, 34 analysis by multiplex polymerase chain reaction, 35 and reactivity with a latex agglutination test specific for C. dubliniensis 36, † † and an anti-C. dubliniensis antiserum. 37 Statistical Analyses Descriptive analyses of data were expressed as mean -SD. Significance of group comparisons was determined by the Student t test or the chi-square test with the Yates' correction when appropriate.…”

Candida albicans or Candida dubliniensis?

“…Bikandi et al [50] demonstrated the possibility of using differences between C. albicans and C. dubliniensis cell wall localised antigens to differentiate between these species. Cross reactivity was, however, observed for C. krusei.…”