Rapid identification of Candida albicans by using Albicans ID and fluoroplate agar plates

Abstract

Two commercially available agar media, Albicans ID and Fluoroplate, that use a chromogenic or a fluorogenic substrate for the detection and identification of Candida albicans were evaluated. From 1,006 clinical samples containing 723 yeast strains, 352 C. albicans strains were detected with either of the two media. The sensitivity of each of the two media was 93.8% and the specificity was 98.6%, with five false-positive reactions for Candida tropicalis and no false-negative reactions.

Oral Microbiology: Isolation and identification of candida from the oral cavity

“…Pagano-Levin agar has a similar sensitivity to SDA but is superior for the detection of more than one species in the sample (Samaranayake et al, 1987). CHROMagar ® Candida identifies C. albicans, C. tropicalis and C. krusei based on colony colour and appearance ( Figure 1b) (Beighton et al, 1995), whilst Albicans ID and Fluroplate have proven beneficial for the presumptive identification of C. albicans (Rousselle et al, 1994). The specificity of identification is reported to be 95% for CHROMagar ® Candida (Pfaller et al, 1996) and 98.6% for Albicans ID and Fluroplate agars (Rousselle et al, 1994).…”

“…CHROMagar ® Candida identifies C. albicans, C. tropicalis and C. krusei based on colony colour and appearance ( Figure 1b) (Beighton et al, 1995), whilst Albicans ID and Fluroplate have proven beneficial for the presumptive identification of C. albicans (Rousselle et al, 1994). The specificity of identification is reported to be 95% for CHROMagar ® Candida (Pfaller et al, 1996) and 98.6% for Albicans ID and Fluroplate agars (Rousselle et al, 1994). The use of CHROMagar Candida as a primary isolation agar has been cited as an approach that permits discrimination of the newly described C. dubliniensis (Sullivan et al, 1995) from C. albicans.…”

Comparison of the new API Candida system to the ID 32C system for identification of clinically important yeast species

“…These systems have some advantages and drawbacks concerning their capacity to rapidly and accurately identify yeast isolates. To improve yeast identification, different techniques have been developed in the last few years: latex tests (17,18), PCR (11), and differential culture media (4,12,15,19). However, for the moment these new procedures have been performed only for a few species and are not adapted to the identification of all medically important yeasts.…”

Inhibitory effect of chromogenic culture media on the growth of Rhodotorula: relevance to the diagnosis of Rhodotorula spp. infections

“…Consequently, when evaluations of this chromogenic culture medium were published, half of the studies did not include Rhodotorula spp in their panel (1,(15)(16)(17)(18)(19)(20)(21)(22)(23) (Table 4). The few that did failed to point out any limitations on growth (2,(24)(25)(26)(27)(28)(29)(30). Different chromogenic substrates are obviously used in CHROMagar Candida and Candi 4, as C. albi-cans appears green on CHROMagar, whereas it appears pink to purple on Candi 4.…”