Oral Microbiology: Isolation and identification of candida from the oral cavity

Williams, David Wynne; Lewis, Michael Alexander Oxenham

doi:10.1111/j.1601-0825.2000.tb00314.x

Cited by 159 publications

(136 citation statements)

References 55 publications

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“…for acrylic resin, to which it will adhere and colonize 3,4 . Effective oral and prosthesis hygiene is therefore essential for the maintenance of tissue viability and prevention of denture associated infections 5,6 .…”

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confidence: 99%

Antifungal activity of components used for decontamination of dental prostheses on the growth of Candida albicans

Gouveia

Freire

Leite

et al. 2014

Rev. odontol. UNESP

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ResumoIntrodução: A efetividade de soluções antimicrobianas empregadas na descontaminação de próteses ainda é incerta. Objetivo: Avaliar a atividade antifúngica de soluções empregadas na descontaminação de próteses sobre o crescimento de Candida albicans. Material e método: Foram avaliados os produtos: Corega Tabs Branqueador  (S1), Hipoclorito de Sódio 1% (S2), Bicarbonato de Sódio 1% (S3), Peróxido de Hidrogênio 1% (S4), Digluconato de Clorexidina 0,12% -Periogard  (S5), Enxaguatório bucal a base de óleos essenciais -Listerine  (S6), e óleo essencial de Rosmarinus officinalis (alecrim) nas concentrações 1% (S7) e 2% (S8). A atividade antifúngica foi avaliada por meio da técnica de difusão em ágar e da determinação da curva de morte microbiana de amostras de C. albicans (ATCC 90028) na concentração 1,5×10 6 UFC/mL. Os testes foram realizados em triplicata e a análise estatística se deu pelos testes ANOVA Two-Way e Tukey, sendo adotado nível de confiança de 95%. Resultado: A média dos halos de inibição do crescimento, em milímetros, obtidos para os produtos foram: 0,0 (S1); 44,7 (S2); 0,0 (S3); 21,6 (S4); 10,0 (S5); 6,1 (S6); 0,0 (S7) e 2,4 (S8). Para curva de morte microbiana, todos os produtos apresentaram diferença estatisticamente significante (p<0,05) do grupo controle (cloreto de sódio 0,85%) e do grupo S3. Verificou-se crescimento fúngico inferior a 2×10 4 UFC/mL e acentuação na curva de morte microbiana após 30 minutos de ação, a exceção do grupo S3. Conclusão: As substâncias analisadas, a exceção do Bicarbonato de Sódio, possuem ação antifúngica frente C. albicans, podendo contribuir para higienização de próteses.Descritores: Produtos com ação antimicrobiana; Candida albicans; descontaminação. AbstractIntroduction: The effectiveness of antimicrobial solutions employed in dental prosthesis decontamination is still uncertain. Aim: To evaluate the antifungal activity of cleaners used in the decontamination of dental prostheses on the growth of Candida albicans. Material and method: The evaluated products were: Corega Tabs  (S1), Sodium Hypochlorite 1% (S2), Sodium Bicarbonate 1% (S3), Hydrogen Peroxide 1% (S4), Chlorhexidine Digluconate 0.12% -Periogard  (S5), Mouthrinse based on essential oils -Listerine  (S6), essential oil from Rosmarinus officinalis (rosemary) at concentrations of 1% (S7) and 2% (S8). The antifungal activity of the products was evaluated by agar diffusion technique and the determination of microbial death curve of samples of C. albicans (ATCC 90028) in concentration 1.5 × 10 6 CFU/mL. The tests were performed in triplicate and statistical analysis was made by ANOVA Two-Way and Tukey tests, with the confidence level of 95%. Result: The average of the zones of inhibition growth, in millimeters, obtained for the products were: 0.0 (S1), 44.7 (S2), 0.0 (S3), 21.6 (S4), 10.0 (S5), 6.1 (S6), 0.0 (S7) and 2.4 (S8). Considering the determination of microbial death curve, all products showed a statistical difference (p<0.01) from control (0.85% sodium chloride) and S3 groups. Fungal growt...

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confidence: 99%

Antifungal activity of components used for decontamination of dental prostheses on the growth of Candida albicans

Gouveia

Freire

Leite

et al. 2014

Rev. odontol. UNESP

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“…Among the many species, Candida albicans is the most important microorganism implicated in fungal infection (1). These microorganisms reside in the oral cavity as part of the normal microflora, however, they can cause disease when the host is predisposed.…”

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Antifungal activity of alpha-mangostin against Candida albicans

2009

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This study was conducted to examine the activity of alpha-mangostin against Candida albicans, the most important microorganism implicated in oral candidiasis. Its activity was compared to Clotrimazole and Nystatin. Results showed that alphamangostin was effective against C. albicans, the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were 1,000 and 2,000 µg/ml, respectively. The C. albicans killing activity of alpha-mangostin was more effective than Clotrimazole and Nystatin. The cytotoxicity of alphamangostin was determined and it was found that alphamangostin at 4,000 µg/ml was not toxic to human gingival fibroblast for 480 min. The strong antifungal activity and low toxicity of alpha-mangostin make it a promising agent for treatment of oral candidiasis. (J Oral Sci 51, [401][402][403][404][405][406] 2009)

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“…The slides stained by the periodic acid-Schiff (PAS) or Grocott methods allow quick and easy identification of all morphological forms of Candida spp. (28,55) .…”

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“…yeasts may be found in the normal flora of the skin and mucosae (21,52) . Besides, it is a method not available in all clinical laboratories, and whose result requires days (15,55) . Biopsy is particularly useful for the diagnosis of chronic hyperplastic candidiasis.…”

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“…Diagnostic methods include direct examination, culture, cytopathologic analysis and the histopathologic examination (5,16,45,46,55) . In direct examination, the material collected from scraping the affected area is placed on a glass slide, and then the addition of a drop of 20% potassium hydroxide (KOH) destroys epithelial cells on the smear, permitting to view yeasts and hyphae, which are more resistant.…”

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