Comparison of the new API Candida system to the ID 32C system for identification of clinically important yeast species

Fricker‐Hidalgo, Hélène; Vandapel, O; Duchesne, M.; Mazoyer, Marie-Andrée; Monget, D.; Lardy, Bernard; Lebeau, B.; Freney, J.; Ambroise-Thomas, P; Grillot, R.

doi:10.1128/jcm.34.7.1846-1848.1996

Cited by 47 publications

(21 citation statements)

References 17 publications

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“…demonstrated that FTIR spectroscopy presents enough discriminating power to identify C. albicans with great sensitivity. The percentage rates in the present study compare favorably with those obtained by the classical methods of identification, which are based on macro‐ and microscopic examination of fungal cultures and biochemical tests, and which give identification rates >90% for the most frequent species of Candida and dermatophytes . These results are also comparable with those obtained in food yeasts by other research teams using conventional FTIR spectroscopy.…”

Section: Discussionsupporting

confidence: 85%

Applicability of Fourier transform infrared (FTIR) spectroscopy in rapid identification of some Candida and dermatophyte species infections in humans

et al. 2016

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Traditional systems of identifying yeasts and dermatophytes have many disadvantages. Preliminary data on a radically different approach based on optical spectroscopic techniques suggest that these techniques may offer some advantages. We conducted a trial to verify the practical applicability of Fourier transform infrared (FTIR) spectroscopy in the identification of some yeast and dermatophyte species, in which samples from 50 patients with superficial fungal infections were cultured on Sabouraud dextrose agar with chloramphenicol and cycloheximide (actidione) and studied using FTIR microspectroscopy. Spectra of the same species were identical, whereas spectra of different species did not show similarity. This study showed that FTIR microspectroscopy is promising and can be used to obtain, with a single measurement, a "molecular fingerprint" of Candida and dermatophyte species. It can be improved further in terms of reliability.

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Section: Discussionsupporting

confidence: 85%

Applicability of Fourier transform infrared (FTIR) spectroscopy in rapid identification of some Candida and dermatophyte species infections in humans

et al. 2016

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“…The conclusion of the authors of this latter study was that although this biotyping method was effective for research applications, it probably would not prove effective for clinical use. In addition to serotyping and the Odds and Abbott biotyping method, a number of other biotyping methods have been used to discriminate C. albicans strains, including morphotyping (145,270,293), resistotyping (144,215), killer yeast typing (278,279), enzyme typing (66,424,425), sugar assimilation typing (50,105,113,120) and drug susceptibility typing (294). Isoenzyme biotyping has also been successfully applied to Candida species (52,180,288).…”

Section: Biotyping Is Inadequate As a Dna Fingerprinting Methodsmentioning

confidence: 99%

The Ins and Outs of DNA Fingerprinting the Infectious Fungi

2000

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SUMMARY DNA fingerprinting methods have evolved as major tools in fungal epidemiology. However, no single method has emerged as the method of choice, and some methods perform better than others at different levels of resolution. In this review, requirements for an effective DNA fingerprinting method are proposed and procedures are described for testing the efficacy of a method. In light of the proposed requirements, the most common methods now being used to DNA fingerprint the infectious fungi are described and assessed. These methods include restriction fragment length polymorphisms (RFLP), RFLP with hybridization probes, randomly amplified polymorphic DNA and other PCR-based methods, electrophoretic karyotyping, and sequencing-based methods. Procedures for computing similarity coefficients, generating phylogenetic trees, and testing the stability of clusters are then described. To facilitate the analysis of DNA fingerprinting data, computer-assisted methods are described. Finally, the problems inherent in the collection of test and control isolates are considered, and DNA fingerprinting studies of strain maintenance during persistent or recurrent infections, microevolution in infecting strains, and the origin of nosocomial infections are assessed in light of the preceding discussion of the ins and outs of DNA fingerprinting. The intent of this review is to generate an awareness of the need to verify the efficacy of each DNA fingerprinting method for the level of genetic relatedness necessary to answer the epidemiological question posed, to use quantitative methods to analyze DNA fingerprint data, to use computer-assisted DNA fingerprint analysis systems to analyze data, and to file data in a form that can be used in the future for retrospective and comparative studies.

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“…Most isolates used in this study were from a clinical collection. All the strains isolated from C. albicans-infected patients were from our hospital and were confirmed by the Microbiological Research Laboratory, the Center of Health Research and Epidemic Prevention, Shandong Province, using published mycological methods (Baumgartner et al, 1996;Fricker-Hidalgo et al, 1996;Pfaller et al, 1996;National Committee for Clinical Laboratory Standards, 2002). Two azole-susceptible isolates, CA8 and CA14, as well as two azole-resistant isolates, CA10 and CA137, were studied.…”

Section: Organism Reagent and Growth Mediummentioning

confidence: 99%

The combination of minocycline and fluconazole causes synergistic growth inhibition against Candida albicans: an in vitro interaction of antifungal and antibacterial agents

Shi

Chen

et al. 2010

FEMS Yeast Research

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Combination therapy can be used for the treatment of fungal infections, especially for those caused by antifungal-resistant fungi. In the present study, in vitro interactions and mechanisms between fluconazole and minocycline against Candida albicans were evaluated. The nature of the interactions determined by spectrophotometric method in a checkerboard assay was interpreted using nonparametric models of fractional inhibitory concentration index (FICI) and percentages of growth difference (ΔE). In the mechanism study, we evaluated the potential activity of minocycline on fluconazole penetrating the C. albicans biofilm. Furthermore, the effect of fluconazole and minocycline alone and in combination on the cellular calcium balance, as well as on the uptake and efflux of fluconazole were evaluated. It was found that fluconazole can work synergistically with minocycline against fluconazole-resistant C. albicans; the minimum inhibitory concentration of fluconazole decreased from 512 to 2 microgmL(-1) when fluconazole and minocycline were given in combination, with an FICI of 0.035 and 0.064 and high-percentage synergistic interactions of 1250% and 988% for the two resistant strains. The mechanism of action was suggested to be the enhancement of minocycline on fluconazole penetrating biofilm, and inducing the intracellular calcium release, instead of impacting on the uptake and efflux of fluconazole. Our results suggest that the combination of fluconazole and minocycline can reduce the fluconazole resistance of C. albicans in vitro.

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Comparison of the new API Candida system to the ID 32C system for identification of clinically important yeast species

Cited by 47 publications

References 17 publications

Applicability of Fourier transform infrared (FTIR) spectroscopy in rapid identification of some Candida and dermatophyte species infections in humans

Applicability of Fourier transform infrared (FTIR) spectroscopy in rapid identification of some Candida and dermatophyte species infections in humans

The Ins and Outs of DNA Fingerprinting the Infectious Fungi

The combination of minocycline and fluconazole causes synergistic growth inhibition against Candida albicans: an in vitro interaction of antifungal and antibacterial agents

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