Rapid Enrichment and Ultrasensitive Detection of Influenza A Virus in Human Specimen using Magnetic Quantum Dot Nanobeads Based Test Strips

Bai, Zikun; Wei, Hongjuan; Yang, Xingsheng; Zhu, Yanhui; Peng, Yongjin; Yang, Jing; Wang, Chongwen; Rong, Zhen; Wang, Shengqi

doi:10.1016/j.snb.2020.128780

Cited by 61 publications

(44 citation statements)

References 28 publications

(33 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Here, it is relevant to mention about the antiviral performance of carbon quantum dots (CQDs), which are categorized as mainly sp 2 graphitic structures with sizes of typically less than 10 nm [238] , [239] .…”

Section: Interactions Between Graphene-based Materials and Virusesmentioning

confidence: 99%

Antiviral performance of graphene-based materials with emphasis on COVID-19: A review

Seifi

Kamali

2021

Medicine in Drug Discovery

View full text Add to dashboard Cite

Coronavirus disease-2019 has been one of the most challenging global epidemics of modern times with a large number of casualties combined with economic hardships across the world. Considering that there is still no definitive cure for the recent viral crisis, this article provides a review of nanomaterials with antiviral activity, with an emphasis on graphene and its derivatives, including graphene oxide, reduced graphene oxide and graphene quantum dots. The possible interactions between surfaces of such nanostructured materials with coronaviruses are discussed. The antiviral mechanisms of graphene materials can be related to events such as the inactivation of virus and/or the host cell receptor, electrostatic trapping and physico-chemical destruction of viral species. These effects can be enhanced by functionalization and/or decoration of carbons with species that enhances graphene-virus interactions. The low-cost and large-scale preparation of graphene materials with enhanced antiviral performances is an interesting research-direction to be explored.

show abstract

Section: Interactions Between Graphene-based Materials and Virusesmentioning

confidence: 99%

Antiviral performance of graphene-based materials with emphasis on COVID-19: A review

Seifi

Kamali

2021

Medicine in Drug Discovery

View full text Add to dashboard Cite

show abstract

“…Therefore, suitable antibodies with affinity and specificity are the key to construct the two-channel ICA. Our previous work screened a pair of efficient mAbs for FluA detection [ 44 ]. The SARS-CoV-2 NP mAbs used in this study were selected as shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Development of an ultrasensitive fluorescent immunochromatographic assay based on multilayer quantum dot nanobead for simultaneous detection of SARS-CoV-2 antigen and influenza A virus

Wang

Yang

Zheng

et al. 2021

Sensors and Actuators B: Chemical

Self Cite

View full text Add to dashboard Cite

“…A mature sample preparation process has been applied to dilute specimens by washing buffers and filter out larger particle complexes. 59 − 61 To avoid the nonspecific binding of unknown chemical compounds from the complex matrix, surface-functionalized MNPs can be PEGylated, 62 − 64 where polyethylene glycol is a hydrophilic and neutrally charged polymer that can help prevent the nonspecific bindings of other chemical compounds to MNP surfaces.…”

Section: Discussionmentioning

confidence: 99%

One-Step, Wash-free, Nanoparticle Clustering-Based Magnetic Particle Spectroscopy Bioassay Method for Detection of SARS-CoV-2 Spike and Nucleocapsid Proteins in the Liquid Phase

Chugh

Krishna

et al. 2021

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

With the ongoing global pandemic of coronavirus disease 2019 (COVID-19), there is an increasing quest for more accessible, easy-to-use, rapid, inexpensive, and high-accuracy diagnostic tools. Traditional disease diagnostic methods such as qRT-PCR (quantitative reverse transcription-PCR) and ELISA (enzyme-linked immunosorbent assay) require multiple steps, trained technicians, and long turnaround time that may worsen the disease surveillance and pandemic control. In sight of this situation, a rapid, one-step, easy-to-use, and high-accuracy diagnostic platform will be valuable for future epidemic control, especially for regions with scarce medical resources. Herein, we report a magnetic particle spectroscopy (MPS) platform for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) biomarkers: spike and nucleocapsid proteins. This technique monitors the dynamic magnetic responses of magnetic nanoparticles (MNPs) and uses their higher harmonics as a measure of the nanoparticles’ binding states. By anchoring polyclonal antibodies (pAbs) onto MNP surfaces, these nanoparticles function as nanoprobes to specifically bind to target analytes (SARS-CoV-2 spike and nucleocapsid proteins in this work) and form nanoparticle clusters. This binding event causes detectable changes in higher harmonics and allows for quantitative and qualitative detection of target analytes in the liquid phase. We have achieved detection limits of 1.56 nM (equivalent to 125 fmole) and 12.5 nM (equivalent to 1 pmole) for detecting SARS-CoV-2 spike and nucleocapsid proteins, respectively. This MPS platform combined with the one-step, wash-free, nanoparticle clustering-based assay method is intrinsically versatile and allows for the detection of a variety of other disease biomarkers by simply changing the surface functional groups on MNPs.

show abstract

Rapid Enrichment and Ultrasensitive Detection of Influenza A Virus in Human Specimen using Magnetic Quantum Dot Nanobeads Based Test Strips

Abstract: Graphical abstract

Cited by 61 publications

References 28 publications

Antiviral performance of graphene-based materials with emphasis on COVID-19: A review

Antiviral performance of graphene-based materials with emphasis on COVID-19: A review

Development of an ultrasensitive fluorescent immunochromatographic assay based on multilayer quantum dot nanobead for simultaneous detection of SARS-CoV-2 antigen and influenza A virus

One-Step, Wash-free, Nanoparticle Clustering-Based Magnetic Particle Spectroscopy Bioassay Method for Detection of SARS-CoV-2 Spike and Nucleocapsid Proteins in the Liquid Phase

Contact Info

Product

Resources

About