Development of an ultrasensitive fluorescent immunochromatographic assay based on multilayer quantum dot nanobead for simultaneous detection of SARS-CoV-2 antigen and influenza A virus

Wang, Chongwen; Yang, Xingsheng; Zheng, Shuai; Cheng, Xiaodan; Xiao, Rui; Liu, Qingjun; Wang, Wenqi; Liu, Xiaoxian; Wang, Shengqi

doi:10.1016/j.snb.2021.130372

Cited by 72 publications

(50 citation statements)

References 56 publications

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“…The detection limit of it was 48 ng/mL SARS-CoV-2 spike antigen. Wang et al (2021) developed a fluorescent immunochromatographic assay for SARS-CoV-2 based on multilayer quantum dot nanobead with a detection limit of 5 pg/mL SARS-CoV-2 NP antigen.…”

Section: Immunological Methodsmentioning

confidence: 99%

Diagnostic techniques for COVID-19: A mini-review

Wei

Chen

Li³

et al. 2022

Journal of Virological Methods

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COVID-19, a new respiratory infectious disease, was first reported at the end of 2019, in Wuhan, China. Now, COVID-19 is still causing major loss of human life and economic productivity in almost all countries around the world. Early detection, early isolation, and early diagnosis of COVID-19 patients and asymptomatic carriers are essential to blocking the spread of the pandemic. This paper briefly reviewed COVID-19 diagnostic assays for clinical application, including nucleic acid tests, immunological methods, and Computed Tomography (CT) imaging. Nucleic acid tests (NAT) target the virus genome and indicates the existence of the SARS-CoV-2 virus. Currently, real-time quantitative PCR (qPCR) is the most widely used NAT and, basically, is the most used diagnostic assay for COVID-19. Besides qPCR, many novel rapid and sensitive NAT assays were also developed. Serological testing (detection of serum antibodies specific to SARS-CoV-2), which belongs to the immunological methods, is also used in the diagnosis of COVID-19. The positive results of serological testing indicate the presence of antibodies specific to SARS-CoV-2 resulting from being infected with the virus. Viral antigen detection assays are also important immunological methods used mainly for rapid virus detection. However, only a few of these assays had been reported. CT imaging is still an important auxiliary diagnosis tool for COVID-19 patients, especially for symptomatic patients in the early stage, whose viral load is low and different to be identified by NAT. These diagnostic techniques are all good in some way and applying a combination of them will greatly improve the accuracy of COVID-19 diagnostics.

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Section: Immunological Methodsmentioning

confidence: 99%

Diagnostic techniques for COVID-19: A mini-review

Wei

Chen

Li³

et al. 2022

Journal of Virological Methods

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“…EDC: N ‐(3‐dimethylaminopropyl)‐ N ′‐ethylcarbodiimide hydrochloride; PEI: polyethyleneimine; sulfo‐NHS: N ‐hydroxysulfosuccinimide. Reproduced with permission from Wang et al 62 …”

Section: Qds Against Sars‐cov‐2: Diagnosis and Inhibitionmentioning

confidence: 99%

“…9). 62 A SARS-CoV-2 antibody-linked magnetic graphene QD-built magnetic relaxation switch was created for the specific recognition of SARS-CoV-2 (Fig. 10(a)).…”

Section: Diagnostic Applicationsmentioning

confidence: 99%

Quantum dots against SARS‐CoV‐2: diagnostic and therapeutic potentials

Rabiee

Ahmadi

Soufi

et al. 2022

J of Chemical Tech & Biotech

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The application of quantum dots (QDs) for detecting and treating various types of coronaviruses is very promising, as their low toxicity and high surface performance make them superior among other nanomaterials; in conjugation with fluorescent probes they are promising semiconductor nanomaterials for the detection of various cellular processes and viral infections. In view of the successful results for inhibiting SARS‐CoV‐2, functional QDs could serve eminent role in the growth of safe nanotherapy for the cure of viral infections in the near future; their large surface areas help bind numerous molecules post‐synthetically. Functionalized QDs with high functionality, targeted selectivity, stability and less cytotoxicity can be employed for highly sensitive co‐delivery and imaging/diagnosis. Besides, due to the importance of safety and toxicity issues, QDs prepared from plant sources (e.g. curcumin) are much more attractive, as they provide good biocompatibility and low toxicity. In this review, the recent developments pertaining to the diagnostic and inhibitory potentials of QDs against SARS‐CoV‐2 are deliberated including important challenges and future outlooks. © 2022 Society of Chemical Industry (SCI).

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“…Nanomaterials can be applied in diagnosis, prophylaxis, and treatment systems to combat infectious diseases [5]. SARS-CoV-2 0.55 fg/mL >5 min [7] Immunological Influenza A Virus (H1N1) 1 PFU/ml 30 min [8] Graphene Immunological JEV/AIV 1 fM/10 fM 1 h [9] AIV 1.6 pg/mL 30 min [10] HIV-1 2.3 × 10 −14 M 1 h [11] Influenza A Virus (H5N1) 25 PFU/mL 15 min [12] Zika Virus 450 pmol/L 5 min [13] AuNPs Optical SARS-CoV-2 0.18 ng/µL >10 min [14] 50 RNA copies per reaction 30 min [15] 4 copies/µL 40 min [16] Hepatitis B virus 100 fg/mL 10-15 min [17] Immunological SARS-CoV-2 370 vp/mL 15 min [18] 0.08 ng/mL 30 min [19] Influenza A Virus 7.8 HAU 30 min [20] Zika Virus 0.82 pmol/L 50 min [21] Quantum dots ELISA Influenza A Virus 22 pfu/mL >35 min [22] Influenza A Virus (H5N1) 0.016 HAU >15 min [23] SARS-CoV-2 5 pg/mL >15 min [24] Immunological HEV3 1.23 fM 20 min [25] SARS-CoV 0.1 pg/mL 1 h [26] Synthetic polymer Immunological Influenza A Virus (H1N1) 5 × 10 3~1 0 4 TCID50 9 min [27] Nanofibers Immunological SARS-CoV-2 0.8 pg/mL 20 min [28] Abbreviations: RDT: rapid diagnosis test, DENV: dengue virus, JEV: Japanese encephalitis virus, AIV: avian influenza virus, HEV3: hepatitis E virus 3, AuNP: gold nanoparticle, HIV: human immunodeficiency virus, ELISA: enzyme-linked immunosorbent assay, LOD: limit of detection, SARS-CoV-2: severe acute respiratory syndrome coronavirus 2, TCID50: 50% tissue culture infectious dose, HAU: haemagglutinin unit.…”

Section: Introductionmentioning

confidence: 99%

Application of Nanomaterials as an Advanced Strategy for the Diagnosis, Prevention, and Treatment of Viral Diseases

et al. 2021

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The coronavirus disease (COVID-19) pandemic poses serious global health concerns with the continued emergence of new variants. The periodic outbreak of novel emerging and re-emerging infectious pathogens has elevated concerns and challenges for the future. To develop mitigation strategies against infectious diseases, nano-based approaches are being increasingly applied in diagnostic systems, prophylactic vaccines, and therapeutics. This review presents the properties of various nanoplatforms and discusses their role in the development of sensors, vectors, delivery agents, intrinsic immunostimulants, and viral inhibitors. Advanced nanomedical applications for infectious diseases have been highlighted. Moreover, physicochemical properties that confer physiological advantages and contribute to the control and inhibition of infectious diseases have been discussed. Safety concerns limit the commercial production and clinical use of these technologies in humans; however, overcoming these limitations may enable the use of nanomaterials to resolve current infection control issues via application of nanomaterials as a platform for the diagnosis, prevention, and treatment of viral diseases.

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Development of an ultrasensitive fluorescent immunochromatographic assay based on multilayer quantum dot nanobead for simultaneous detection of SARS-CoV-2 antigen and influenza A virus

Cited by 72 publications

References 56 publications

Diagnostic techniques for COVID-19: A mini-review

Diagnostic techniques for COVID-19: A mini-review

Quantum dots against SARS‐CoV‐2: diagnostic and therapeutic potentials

Application of Nanomaterials as an Advanced Strategy for the Diagnosis, Prevention, and Treatment of Viral Diseases

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